{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:55:48Z","timestamp":1790740548160,"version":"4.1.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T00:00:00Z","timestamp":1679875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Financing Code 001 and Financing Code 88881.134760\/2016-01"],"award-info":[{"award-number":["Financing Code 001 and Financing Code 88881.134760\/2016-01"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Programa Cooperativo sobre Prote\u00e7\u00e3o Florestal\/PROTEF do Instituto de Pesquisas e Estudos Florestais\/IPEF"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Insect pests introduced in eucalyptus plantations in Brazil are mostly of Australian origin, but native microorganisms have potential for their management. High quality biopesticide production based on entomopathogenic fungi depends on adequate technologies. The objective of this study was to evaluate Mycoharvester\u00ae equipment to harvest and separating particles to obtain pure<jats:italic>Metarhizium anisopliae<\/jats:italic>conidia to manage<jats:italic>Thaumastocoris peregrinus<\/jats:italic>Carpintero &amp; Dellap\u00e9, 2006 (Hemiptera: Thaumastocoridae). The Mycoharvester\u00ae version 5b harvested and separated<jats:italic>M<\/jats:italic>.<jats:italic>anisopliae<\/jats:italic>spores. The pure conidia were suspended in Tween 80\u00ae (0.1%) and calibrated to the concentrations of 1 x 10<jats:sup>6<\/jats:sup>, 10<jats:sup>7<\/jats:sup>, 10<jats:sup>8<\/jats:sup>and 10<jats:sup>9<\/jats:sup>conidia\/ml to evaluate the pathogenicity, lethal concentration 50 and 90 (LC<jats:sub>50<\/jats:sub>, LC<jats:sub>90<\/jats:sub>) and lethal time 50 and 90 (LT<jats:sub>50<\/jats:sub>, LT<jats:sub>90<\/jats:sub>) of this fungus to<jats:italic>T<\/jats:italic>.<jats:italic>peregrinus<\/jats:italic>. This equipment harvested 85% of the conidia from rice, with production of 4.8 \u00b1 0.38 x 10<jats:sup>9<\/jats:sup>conidia\/g dry mass of substrate + fungus. The water content of 6.36% of the single spore powder (pure conidia) separated by the Mycoharvester\u00ae was lower than that of the agglomerated product. The product harvested at the concentrations of 10<jats:sup>8<\/jats:sup>and 10<jats:sup>9<\/jats:sup>conidia\/ml caused high mortality to<jats:italic>T<\/jats:italic>.<jats:italic>peregrinus<\/jats:italic>third instar nymphs and adults. The separation of conidia produced by solid-state fermentation with the Mycoharvester\u00ae is an important step toward optimizing the fungal production system of pure conidia, and to formulate biopesticides for insect pest management.<\/jats:p>","DOI":"10.1371\/journal.pone.0283543","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T17:31:11Z","timestamp":1679938271000},"page":"e0283543","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["From the dual cyclone harvest performance of single conidium powder to the effect of Metarhizium anisopliae on the management of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)"],"prefix":"10.1371","volume":"18","author":[{"given":"Simone Graziele Moio","family":"Velozo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murilo Rodrigues","family":"Velozo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9026-578X","authenticated-orcid":true,"given":"Maur\u00edcio Magalh\u00e3es","family":"Domingues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7646-9110","authenticated-orcid":true,"given":"Luciane Katarine","family":"Becchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa Rafaela de","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Raimundo de Souza","family":"Passos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Cola","family":"Zanuncio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0477-4252","authenticated-orcid":true,"given":"Jos\u00e9 Eduardo","family":"Serr\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietrich","family":"Stephan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Frederico","family":"Wilcken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,3,27]]},"reference":[{"key":"pone.0283543.ref001","unstructured":"IB\u00c1. 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