{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:53:37Z","timestamp":1791420817469,"version":"4.3.3"},"reference-count":89,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T00:00:00Z","timestamp":1684800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007329","name":"Universidad del Valle","doi-asserted-by":"publisher","award":["CI 1920 Internal Grant Scheme 2020"],"award-info":[{"award-number":["CI 1920 Internal Grant Scheme 2020"]}],"id":[{"id":"10.13039\/501100007329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Intestinal parasitic infections have been considered a relevant public health problem due to the increased incidence worldwide. In developing countries, diarrhea and gastrointestinal symptoms cause impaired work capacity in adults and delayed rate growth in children. Enteric infections of unknown etiology can often lead to misdiagnosis, increased transmission, and morbidity. The aim of this study was to determine the prevalence of intestinal parasites in a young adult population and their pets. Stool samples from 139 university students and 44 companion animals were subjected to microscopy diagnosis using wet mounts, concentration by zinc sulphate flotation and staining techniques (Kinyoun and trichrome stain). Molecular diagnosis of protozoa was also performed by conventional PCR. The mean age was 24 years, 54% individuals were female, 46% were men, and 66% had at least one pet. The overall prevalence for at least one parasite was 74.8% and the rate of polyparasitism was 37.5%. Eighty-three patients (59.7%) were positive for <jats:italic>Blastocystis<\/jats:italic> spp., followed by <jats:italic>Cryptosporidium<\/jats:italic> spp. 24.5%, <jats:italic>Endolimax nana<\/jats:italic> 13.6%, <jats:italic>Entamoeba dispar\/E<\/jats:italic>. <jats:italic>moshkovskii<\/jats:italic> 7.8% and <jats:italic>Giardia intestinalis<\/jats:italic> 1.4%. Molecular diagnosis substantially improved <jats:italic>Cryptosporidium<\/jats:italic> spp. and <jats:italic>Blastocystis<\/jats:italic> spp. detection and allowed to distinguish <jats:italic>E<\/jats:italic>. <jats:italic>histolytica<\/jats:italic> from commensals in the <jats:italic>Entamoeba<\/jats:italic> complex. Student\u2019s pets were also examined for parasitism. Samples from 27 dogs, 15 cats, one rabbit and one hen were analyzed, and parasites were detected in 30 (68.2%) as follows: <jats:italic>Cryptosporidium<\/jats:italic> spp. (24) <jats:italic>Giardia<\/jats:italic> spp. (4), hookworm (3), <jats:italic>Endolimax nana<\/jats:italic> (2) and <jats:italic>Toxoplasma gondii<\/jats:italic> (1). Overall, university students showed high prevalence of parasitism and polyparasitism suggesting exposure to parasite infected animals and contaminated environments. <jats:italic>Cryptosporidium<\/jats:italic> spp. was the predominant pathogen in human and domestic animals, and it was only detected by PCR, pointing out the need for sensitive tests in diagnosis and surveillance. Control strategies to prevent the effects of parasitic infections in young population should consider pets as reservoirs and transmission source.<\/jats:p>","DOI":"10.1371\/journal.pone.0283824","type":"journal-article","created":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T17:33:34Z","timestamp":1684863214000},"page":"e0283824","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Molecular diagnosis of intestinal protozoa in young adults and their pets in Colombia, South America"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6125-8190","authenticated-orcid":true,"given":"Caterine","family":"Potes-Morales","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria del 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Chavez-Ruvalcaba","year":"2021","journal-title":"Helminthologia"},{"issue":"11","key":"pone.0283824.ref003","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1080\/17474124.2020.1806711","article-title":"Human intestinal parasitic infection: a narrative review on global prevalence and epidemiological insights on preventive, therapeutic and diagnostic strategies for future perspectives","volume":"14","author":"LW Wong","year":"2020","journal-title":"Expert Rev Gastroenterol Hepatol"},{"issue":"6","key":"pone.0283824.ref004","doi-asserted-by":"crossref","first-page":"437","DOI":"10.2217\/fmb-2019-0273","article-title":"Update on the major imported helminth infections in travelers and migrants","volume":"15","author":"FF Norman","year":"2020","journal-title":"Future Microbiol"},{"issue":"4","key":"pone.0283824.ref005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jtm\/taac011","article-title":"Persistent abdominal symptoms in returning travellers: Clinical and molecular findings","volume":"29","author":"S Gefen-Halevi","year":"2022","journal-title":"J Travel Med"},{"issue":"7","key":"pone.0283824.ref006","doi-asserted-by":"crossref","first-page":"e2865","DOI":"10.1371\/journal.pntd.0002865","article-title":"The Global Burden of Disease Study 2010: Interpretation and Implications for the Neglected Tropical Diseases","volume":"8","author":"PJ Hotez","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"pone.0283824.ref007","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-7-37","article-title":"Global numbers of infection and disease burden of soil transmitted helminth infections in 2010","volume":"7","author":"RL Pullan","year":"2014","journal-title":"Parasites and Vectors"},{"issue":"10100","key":"pone.0283824.ref008","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1016\/S0140-6736(17)32130-X","article-title":"Global, regional, and national disability-adjusted life-years (DALYs) for 333 diseases and injuries and healthy life expectancy (HALE) for 195 countries and territories, 1990\u20132016: A systematic analysis for the Global Burden of Disease Study 2016","volume":"390","author":"SI Hay","year":"2017","journal-title":"Lancet"},{"issue":"6","key":"pone.0283824.ref009","first-page":"703","article-title":"Understanding DALYs (disability-adjusted life years)","volume":"16","author":"CJ Murray","year":"1997","journal-title":"J Health Econ"},{"issue":"7","key":"pone.0283824.ref010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0010575","article-title":"Reduction in DALYs lost due to soil-transmitted helminthiases and schistosomiasis from 2000 to 2019 is parallel to the increase in coverage of the global control programmes","volume":"16","author":"A Montresor","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"pone.0283824.ref011","doi-asserted-by":"crossref","first-page":"882","DOI":"10.2478\/s11686-020-00230-1","article-title":"Prevalence of Cryptosporidium infection in the global population: A systematic review and meta-analysis","volume":"65","author":"S Dong","year":"2020","journal-title":"Acta Parasitol"},{"issue":"2","key":"pone.0283824.ref012","doi-asserted-by":"crossref","first-page":"426","DOI":"10.3390\/microorganisms10020426","article-title":"Prevalence of intestinal parasitoses in a non-endemic setting during a 10-year period (2011\u20132020): A focus on Dientamoeba fragilis","volume":"10","author":"A Calderaro","year":"2022","journal-title":"Microorganisms"},{"issue":"June","key":"pone.0283824.ref013","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.actatropica.2017.06.029","article-title":"Molecular epidemiology of Dientamoeba fragilis","volume":"184","author":"SM Cacci\u00f2","year":"2018","journal-title":"Acta Trop"},{"issue":"23","key":"pone.0283824.ref014","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232315407","article-title":"A Cross-Sectional Study on the Occurrence of the Intestinal Protist, Dientamoeba fragilis, in the Gut-Healthy Volunteers and Their Animals","volume":"23","author":"M Jirk\u016f","year":"2022","journal-title":"Int J Mol Sci"},{"issue":"3","key":"pone.0283824.ref015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00284-021-02756-7","article-title":"Relationship among Blastocystis, the Firmicutes\/Bacteroidetes ratio and chronic stress in Mexican University students","volume":"79","author":"JO Guangorena-G\u00f3mez","year":"2022","journal-title":"Curr Microbiol"},{"issue":"3","key":"pone.0283824.ref016","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.pt.2019.12.008","article-title":"Blastocystis","volume":"36","author":"CR Stensvold","year":"2020","journal-title":"Trends Parasitol"},{"key":"pone.0283824.ref017","unstructured":"Pan American Health Organization. Epidemiological profiles of Neglected Diseases and other infections related to poverty in the Latin America and the Caribbean [Internet]. 2009. https:\/\/www.paho.org\/es\/node\/45762"},{"key":"pone.0283824.ref018","unstructured":"Ministerio de Salud y Protecci\u00f3n U de A. Encuesta nacional de parasitismo intestinal en poblaci\u00f3n escolar 2012\u20132014. [Internet]. Medellin; 2015. https:\/\/www.minsalud.gov.co\/sites\/rid\/Lists\/BibliotecaDigital\/RIDE\/VS\/PP\/ET\/%0Aencuesta-nacional-de-parasitismo-2012-2014.pdf."},{"issue":"12 December","key":"pone.0283824.ref019","first-page":"1","article-title":"The accuracy of nucleic acid amplification tests (NAATs) in detecting human intestinal nematode infections: A protocol for a systematic review and meta-analysis","volume":"17","author":"NK Jayakody","year":"2022","journal-title":"PLoS One"},{"issue":"10","key":"pone.0283824.ref020","article-title":"Diagnostic Methods of Common Intestinal Protozoa: Current and Future Immunological and Molecular Methods","volume":"7","author":"LE Fitri","year":"2022","journal-title":"Trop Med Infect Dis"},{"key":"pone.0283824.ref021","unstructured":"World Health Organization. Bench aids for the diagnosis of intestinal parasites. 2019. 32 p."},{"key":"pone.0283824.ref022","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.mimet.2015.01.016","article-title":"Comparison of four rapid diagnostic tests, ELISA, microscopy and PCR for the detection of Giardia lamblia, Cryptosporidium spp. and Entamoeba histolytica in feces","volume":"110","author":"D Van den Bossche","year":"2015","journal-title":"J Microbiol Methods"},{"issue":"1","key":"pone.0283824.ref023","first-page":"24","article-title":"Synchronous identification of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. in stool samples using a multiplex PCR assay","volume":"13","author":"A Bairami","year":"2018","journal-title":"Iran J Parasitol"},{"issue":"1","key":"pone.0283824.ref024","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.ijpara.2004.09.003","article-title":"Detection of Dientamoeba fragilis in fresh stool specimens using PCR","volume":"35","author":"D Stark","year":"2005","journal-title":"Int J Parasitol"},{"issue":"5","key":"pone.0283824.ref025","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1645\/GE-840R.1","article-title":"Detection of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction","volume":"92","author":"R Stensvold","year":"2006","journal-title":"J Parasitol"},{"key":"pone.0283824.ref026","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2180-7-47","article-title":"A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samples","volume":"7","author":"K Khairnar","year":"2007","journal-title":"BMC Microbiol"},{"issue":"3","key":"pone.0283824.ref027","doi-asserted-by":"crossref","first-page":"276","DOI":"10.11613\/BM.2012.031","article-title":"Lessons in biostatistics interrater reliability: the kappa statistic","volume":"22","author":"ML McHugh","year":"2012","journal-title":"Biochem Medica"},{"key":"pone.0283824.ref028","doi-asserted-by":"crossref","DOI":"10.1155\/2021\/8897935","article-title":"A five-year trend of intestinal parasite prevalence among students attending clinic at University of Gondar, Northwest Ethiopia","volume":"2021","author":"A Derso","year":"2021","journal-title":"J Parasitol Res"},{"issue":"2","key":"pone.0283824.ref029","doi-asserted-by":"crossref","first-page":"1","DOI":"10.9734\/IJTDH\/2016\/23886","article-title":"The prevalence of intestinal parasites among students in Federal University of Technology Akure, Nigeria","volume":"14","author":"O Afolabi","year":"2016","journal-title":"Int J Trop Dis Heal"},{"issue":"2","key":"pone.0283824.ref030","doi-asserted-by":"crossref","first-page":"239","DOI":"10.3329\/jasbs.v39i2.17863","article-title":"Occurrence of intestinal parasites among the teachers, students and staffs of Dhaka 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Valle"},{"issue":"1","key":"pone.0283824.ref034","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12879-019-4095-z","article-title":"Prevalence of feco-oral transmitted protozoan infections and associated factors among university students in Ethiopia: A cross-sectional study","volume":"19","author":"BH Ayele","year":"2019","journal-title":"BMC Infect Dis"},{"issue":"12","key":"pone.0283824.ref035","doi-asserted-by":"crossref","first-page":"0","DOI":"10.1016\/j.heliyon.2020.e05729","article-title":"Blastocystis infection frequency and subtype distribution in university students","volume":"6","author":"MR P\u00e9rez","year":"2020","journal-title":"Heliyon"},{"issue":"1","key":"pone.0283824.ref036","doi-asserted-by":"crossref","first-page":"33","DOI":"10.22354\/in.v23i1.753","article-title":"Screening for intestinal parasites in adults from three different regions of Colombia","volume":"23","author":"HC Restrepo","year":"2018","journal-title":"Infectio"},{"issue":"1","key":"pone.0283824.ref037","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12879-019-3810-0","article-title":"Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: A cross-sectional study","volume":"19","author":"X Villamizar","year":"2019","journal-title":"BMC Infect Dis"},{"issue":"3","key":"pone.0283824.ref038","first-page":"907","article-title":"Urban versus Rural prevalence of intestinal parasites using multi-parallel qPCR in Colombia","volume":"104","author":"PE Bryan","year":"2021","journal-title":"Am J Trop Med Hyg"},{"issue":"3","key":"pone.0283824.ref039","first-page":"92","article-title":"Parasitismo intestinal en poblaciones con alto grado de vulnerabilidad del Caribe colombiano","volume":"70","author":"LA Sarmiento-Rubiano","year":"2018","journal-title":"Rev Cubana Med Trop"},{"issue":"1","key":"pone.0283824.ref040","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1590\/S0124-00642012000100013","article-title":"Intestinal parasitism prevalence amongst children from six indigenous communities residing in Cali, Colombia","volume":"14","author":"M Salcedo-Cifuentes","year":"2012","journal-title":"Rev Salud P\u00fablica"},{"issue":"9","key":"pone.0283824.ref041","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/jcm9092786","article-title":"High prevalence of intestinal pathogens in indigenous in Colombia","volume":"9","author":"S Kann","year":"2020","journal-title":"J Clin Med"},{"issue":"1","key":"pone.0283824.ref042","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12889-018-5978-4","article-title":"Prevalence and risk factors for intestinal parasitic infections in pregnant women residing in three districts of Bogot\u00e1, Colombia","volume":"18","author":"AF Espinosa Aranzales","year":"2018","journal-title":"BMC 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Y. Ma","year":"2022","journal-title":"Sci Total Environ"},{"key":"pone.0283824.ref045","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodcont.2021.108656","article-title":"Global prevalence of intestinal protozoan contamination in vegetables and fruits: A systematic review and meta-analysis","volume":"133","author":"M Badri","year":"2022","journal-title":"Food Control"},{"issue":"December 2019","key":"pone.0283824.ref046","first-page":"1","article-title":"A historical review of the techniques of recovery of parasites for their detection in human stools","volume":"53","author":"FA Soares","year":"2020","journal-title":"Rev Soc Bras Med Trop"},{"issue":"4","key":"pone.0283824.ref047","doi-asserted-by":"crossref","first-page":"519","DOI":"10.7705\/biomedica.v36i4.2799","article-title":"Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites","volume":"36","author":"E. J. In\u00eas","year":"2016","journal-title":"Biomedica"},{"issue":"1","key":"pone.0283824.ref048","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2334-14-164","article-title":"Children of Senegal River Basin show the highest prevalence of Blastocystis sp. ever observed worldwide","volume":"14","author":"D El Safadi","year":"2014","journal-title":"BMC Infect Dis"},{"issue":"9","key":"pone.0283824.ref049","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/microorganisms8091408","article-title":"Prevalence and subtype distribution of Blastocystis sp. in senegalese school children","volume":"8","author":"S Khaled","year":"2020","journal-title":"Microorganisms"},{"issue":"10","key":"pone.0283824.ref050","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1111\/risa.12541","article-title":"Cryptosporidium infection risk: results of new dose-response modeling","volume":"36","author":"MJ Messner","year":"2016","journal-title":"Risk 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Med"},{"issue":"19","key":"pone.0283824.ref054","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/AEM.01250-20","article-title":"Veterinary students have a higher risk of contracting cryptosporidiosis when calves with high fecal cryptosporidium loads are used for fetotomy exercises","volume":"86","author":"D Thomas-Lopez","year":"2020","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"pone.0283824.ref055","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-015-0714-8","article-title":"Molecular characterization of intestinal protozoa in two poor communities in the State of S\u00e3o Paulo, Brazil","volume":"8","author":"\u00c9B David","year":"2015","journal-title":"Parasites and Vectors"},{"key":"pone.0283824.ref056","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.actatropica.2016.12.014","article-title":"Diagnosis of intestinal parasites in a rural community of Venezuela: Advantages and disadvantages of using microscopy or 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and genotype","volume":"16","author":"AGL De Oliveira","year":"2021","journal-title":"PLoS One"},{"key":"pone.0283824.ref082","first-page":"100690","article-title":"Cryptosporidium species in non-human animal species in Latin America: Systematic review and meta-analysis","volume":"29","author":"FT Nakashima","year":"2022","journal-title":"Vet Parasitol Reg Stud Reports"},{"issue":"5","key":"pone.0283824.ref083","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1007\/s00436-018-5818-6","article-title":"Genetic uniqueness of Cryptosporidium parvum from dairy calves in Colombia","volume":"117","author":"C Avenda\u00f1o","year":"2018","journal-title":"Parasitol Res"},{"issue":"3","key":"pone.0283824.ref084","first-page":"159","article-title":"Primer reporte de Cryptosporidium parvum en terneros hostein (Bos Taurus) de Manizales, Caldas, Colombia","volume":"59","author":"RJ Ocampo","year":"2012","journal-title":"Rev Med Vet 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