{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:39:20Z","timestamp":1788446360517,"version":"build-2803163510"},"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T00:00:00Z","timestamp":1692230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["32060519"],"award-info":[{"award-number":["32060519"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Scientific and Technological Innovation Guidance Award Fund Project of Inner Mongolia"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> (<jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic>) is a zoonotic parasite that can cause serious pathology in intermediate hosts such as humans and animals. Eating undercooked or raw meat is the most important route of infection by <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic>. Sheep are an important source of meat worldwide, and they are also susceptible to <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic>. Mutton infected with <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic> poses a serious threat to the food safety of consumers. At present, studies have mainly focused on the infection ratio of <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic> in livestock; however, systematic studies have not been performed on differences in the distribution of this parasite in different muscle and viscera tissues of animals. In this study, the differences in the distribution of <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic> in naturally infected Small-tailed Han sheep was studied. By amplifying the B1 gene of the parasite via real-time fluorescence quantification PCR (RT\u2012qPCR), we found that the parasite burden of <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic> differed among different parts of the sheep, with the highest burden observed in the heart among the viscera and the external ridge among the <jats:italic>muscle<\/jats:italic>. The relative expression was ranked from high to low in our study as follows: heart, spleen, external ridge, tenderloin, lung, liver, kidney, neck meat, forelegs, cucumber strips, hind leg, lamb belly, and lamb chops. This study provided important guidance for monitoring the food safety of mutton products.<\/jats:p>","DOI":"10.1371\/journal.pone.0283867","type":"journal-article","created":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T17:30:20Z","timestamp":1692293420000},"page":"e0283867","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Differences in Toxoplasma gondii distribution in different muscle and viscera of naturally infected 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