{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T13:53:15Z","timestamp":1781099595793,"version":"3.54.1"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T00:00:00Z","timestamp":1681084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["491094227"],"award-info":[{"award-number":["491094227"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005629","name":"Stiftung Tier\u00e4rztliche Hochschule Hannover","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005629","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Steroid responsive meningitis arteritis (SRMA) is an aberrant Th2-mediated systemic inflammatory disease in dogs. The etiopathogenesis still remains unclear as no triggering pathogen or autoantigen could be found so far. <jats:bold>Hypothesis<\/jats:bold>. Large high-density peptide microarrays are a suitable screening method to detect possible autoantigens which might be involved in the pathogenesis of SRMA. <jats:bold>Methods<\/jats:bold>. The IgA and IgG profile of pooled serum samples of 5 dogs with SRMA and 5 dogs with neck pain due to intervertebral disc herniation (IVDH) without ataxia or paresis were compared via commercially available high-density peptide microarrays (Discovery Microarray) containing 29,240 random linear peptides. Canine distemper virus nucleoprotein (CDVN) served as positive control as all dogs were vaccinated. Common motifs were compared to amino acid sequences of known proteins via databank search. One suitable protein was manually selected for further analysis with a smaller customized high-density peptide microarray. <jats:bold>Results<\/jats:bold>. Pooled serum of dogs with SRMA and IVDH showed different IgA and IgG responses on Discovery Microarray. Only top IgG responses of dogs with SRMA showed a common motif not related to the control protein CDVN. This common motif is part of the interleukin 1 receptor antagonist protein (IL1Ra). On IL1Ra, dogs with SRMA displayed IgA binding to an additional epitope, which dogs with IVDH did not show. <jats:bold>Discussion<\/jats:bold>. IL1Ra is an anti-inflammatory acute phase protein. Different immunoglobulin binding patterns on IL1Ra could be involved in the pathogenesis of SRMA and IL1Ra might be developed as future biomarker for SRMA.<\/jats:p>","DOI":"10.1371\/journal.pone.0284010","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T17:28:47Z","timestamp":1681147727000},"page":"e0284010","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immunoglobulin profiling with large high-density peptide microarrays as screening method to detect candidate proteins for future biomarker detection in dogs with steroid-responsive meningitis-arteritis"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1427-2555","authenticated-orcid":true,"given":"Jasmin Nicole","family":"Nessler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Tipold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,4,10]]},"reference":[{"issue":"2","key":"pone.0284010.ref001","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0034-5288(95)90060-8","article-title":"Neuroimmunological studies in steroid-responsive meningitis-arteritis in dogs","volume":"58","author":"A Tipold","year":"1995","journal-title":"Res Vet Sci"},{"issue":"5","key":"pone.0284010.ref002","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1111\/j.1939-1676.2008.0164.x","article-title":"Concentrations of acute-phase proteins in dogs with steroid responsive meningitis-arteritis","volume":"22","author":"A Bathen-Noethen","year":"2008","journal-title":"J Vet Intern Med"},{"issue":"1","key":"pone.0284010.ref003","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.tvjl.2008.05.001","article-title":"The role of acute phase proteins in diagnosis and management of steroid-responsive meningitis arteritis in dogs","volume":"182","author":"M Lowrie","year":"2009","journal-title":"Vet J"},{"issue":"2","key":"pone.0284010.ref004","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.tvjl.2010.12.018","article-title":"Determination of immunoglobulin A concentrations in the serum and cerebrospinal fluid of dogs: an estimation of its diagnostic value in canine steroid-responsive meningitis-arteritis","volume":"191","author":"A Maiolini","year":"2012","journal-title":"Vet J"},{"issue":"3","key":"pone.0284010.ref005","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/j.1748-5827.2009.00848.x","article-title":"An update on steroid responsive meningitis-arteritis","volume":"51","author":"A Tipold","year":"2010","journal-title":"J Small Anim Pract"},{"issue":"5","key":"pone.0284010.ref006","first-page":"514","article-title":"Gibt es einen Superantigeneinfluss bei steril eitriger Meningitis-Arteriitis des Hundes? 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