{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:02:03Z","timestamp":1787022123976,"version":"build-2736575974"},"reference-count":74,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T00:00:00Z","timestamp":1680825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Latin American Poultry Association"},{"name":"Latin American Poultry Association"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Environmental enrichment can increase the occurrence of natural behavior and improve leg health and other animal welfare outcomes in broiler chickens. This study aimed to assess the effects of three environmental enrichments, specifically hay bales, step platforms, and laser lights, on subclinical spondylolisthesis prevalence, productivity, behavior, and gait of broiler chickens (\n                    <jats:italic>Gallus gallus domesticus<\/jats:italic>\n                    ). Twenty-four hundred day-old male Ross\u00ae AP95 chicks from a commercial hatchery were used in a completely randomized design with four treatments and four replicate pens per treatment. Pens contained either a Control (C) treatment, an environment similar to a commercial broiler chicken system without environmental enrichments, or an environment with either additional hay bales (HB), additional step platforms (SP), or additional laser lights (LL). Performance, yield, behavior (frequencies), gait score, and subclinical spondylolisthesis prevalences were assessed. When raised with SP or LL access, fewer chickens had subclinical spondylolisthesis than chickens without enrichments (C) or with HB access. Chickens with access to SP exhibited higher wing yield and less abdominal fat than animals from the C group. Chickens from the LL and HB treatments explored more and rested less frequently than animals from the C and SP treatments. As chickens aged, they became less active, exploring less and increasing resting and comfort behaviors. Treatments did not affect gait. Gait was not associated with subclinical spondylolisthesis prevalence. Environmental enrichments benefitted chicken health (subclinical spondylolisthesis) and behavior (exploration) without negative consequences for performance and yield.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0284087","type":"journal-article","created":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T13:33:42Z","timestamp":1680874422000},"page":"e0284087","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Providing environmental enrichments can reduce subclinical spondylolisthesis prevalence without affecting performance in broiler chickens"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3636-4805","authenticated-orcid":true,"given":"Marconi Italo","family":"Louren\u00e7o da Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibiara Correia de Lima","family":"Almeida Paz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andressa Silva","family":"Jacinto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Antonio","family":"Nascimento Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3754-8948","authenticated-orcid":true,"given":"Ana Beatriz Santos de","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid Grazieli Althman dos","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9326-3456","authenticated-orcid":true,"given":"Francine dos Santos","family":"Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana Ribeiro","family":"Caldara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonie","family":"Jacobs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,4,7]]},"reference":[{"key":"pone.0284087.ref001","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0168-1591(00)00105-2","article-title":"Early locomotor behaviour in genetic stocks of chickens with different growth rates","volume":"68","author":"D Bizeray","year":"2000","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0284087.ref002","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0168-1591(99)00102-1","article-title":"The behaviour of broiler chickens and its modification by lameness","volume":"67","author":"CA Weeks","year":"2000","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0284087.ref003","article-title":"Equilibrium condition during locomotion and gait in broiler chickens","volume":"18","author":"MCF Alves","year":"2016","journal-title":"Brazilian J Poult Sci"},{"key":"pone.0284087.ref004","first-page":"263","article-title":"Pathomorphologial investigation of spondylolisthesis leaded to spondylosis in commercial broiler chicken with posterior paralysis: A case study","volume":"12","author":"BM Abbasabadi","year":"2021","journal-title":"Vet Res Forum"},{"key":"pone.0284087.ref005","doi-asserted-by":"crossref","DOI":"10.1111\/j.1751-0813.1971.tb02115.x","article-title":"Occurrence of spondylolisthesis (kinky-back) in broiler chickens in South Australia","volume":"47","author":"WR Kelly","year":"1971","journal-title":"Aust Vet J"},{"key":"pone.0284087.ref006","doi-asserted-by":"crossref","first-page":"283","DOI":"10.2141\/jpsa.0120144","article-title":"Pathomorphological investigations on the incidence of axial skeleton pathology associated with posterior paralysis in commercial broiler chickens","volume":"50","author":"I. 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