{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T15:26:38Z","timestamp":1767108398298,"version":"3.40.5"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Cutaneous leishmaniasis (CL) is a prevalent infectious disease with considerable morbidity annually. Here, we aimed to investigate the likely variations in gene expression of glycoprotein63 (gp63), heat shock protein 70 (HSP70), histone, arginase, cysteine protease B (CPB), <jats:italic>Leishmania<\/jats:italic> homologue of receptors for activated C kinase (LACK), small hydrophilic endoplasmic reticulum-associated protein (SHERP) in metacyclic promastigotes of <jats:italic>L<\/jats:italic>. <jats:italic>major<\/jats:italic> isolated from <jats:italic>Phlebotomus papatasi<\/jats:italic> sand flies and promastigotes excessively cultured in culture medium. The parasites were collected from suspected CL cases in Pasteur Institute of Iran, cultured and inoculated into the female BALB\/c mice (2\u00d710<jats:sup>6<\/jats:sup> promastigotes). Sand flies were trapped in Qom province, fed with the blood of euthanized infected mice and subsequently dissected in order to isolate the midgut including stomodeal valve. The metacyclic promastigotes were isolated from <jats:italic>Ph<\/jats:italic>. <jats:italic>papatasi<\/jats:italic> (Pro-Ppap) using peanut agglutinin test (PNA), then continuously cultured in RPMI-1640 medium enriched with fetal bovine serum, penicillin (100 U\/ml) and streptomycin (100 mg\/ml) to reach stationary phase (Pro-Stat). The gene expression was evaluated in both parasitic stages (Pro-Ppap and Pro-Stat) using qRT-PCR. Out results showed a significant increased gene expression at Pro-Ppap stage for gp63 (<jats:italic>P<\/jats:italic> = 0.002), SHERP (<jats:italic>P<\/jats:italic> = 0.001) and histone (<jats:italic>P<\/jats:italic> = 0.026) genes, in comparison with Pro-Stat stage. Noticeably, significant changes were, also, demonstrated in 10<jats:sup>th<\/jats:sup> to 15<jats:sup>th<\/jats:sup> passages [gp63 (<jats:italic>P<\/jats:italic> = 0.041), arginase (<jats:italic>P<\/jats:italic> = 0.016), LACK (<jats:italic>P<\/jats:italic> = 0.025)] and in 5<jats:sup>th<\/jats:sup> to 20<jats:sup>th<\/jats:sup> passage (SHERP) (<jats:italic>P<\/jats:italic> = 0.029). In conclusion, the findings of the present study seem to be essential in designing <jats:italic>Leishmania<\/jats:italic> studies, in particular regarding host-parasite interaction, immunization and infectivity studies.<\/jats:p>","DOI":"10.1371\/journal.pone.0284240","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T17:39:08Z","timestamp":1681407548000},"page":"e0284240","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of expression variations in virulence-related genes of Leishmania major after several culture passages compared with Phlebotomus papatasi isolated promastigotes"],"prefix":"10.1371","volume":"18","author":[{"given":"Taher","family":"Nemati Haravani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parviz","family":"Parvizi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed Hossein","family":"Hejazi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Mehdi","family":"Sedaghat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7836-1293","authenticated-orcid":true,"given":"Abbasali","family":"Eskandarian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud","family":"Nateghi 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