{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T00:50:00Z","timestamp":1775263800111,"version":"3.50.1"},"reference-count":27,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T00:00:00Z","timestamp":1681862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Candida albicans<\/jats:italic> can cause two major types of infections: superficial infection and systemic candidiasis. <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> infects diverse host niches, owing to a wide range of virulence factors and attributes, such as morphological transitions and phenotypic switching. <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> uses glycolysis, followed by alcoholic fermentation or mitochondrial respiration to rapidly generate ATP under aerobic conditions. In this study, we quantified the mRNA expression of several glycolysis-related enzymes associated with the initial phase of environmental changes using two strains: a type strain, NBRC 1385, and a strain from a patient with auto-brewery syndrome, LSEM 550. Additionally, we analyzed the regulation of a rate-limiting enzyme in glycolysis, phosphofructokinase 1 (PFK1). Our results showed that the mRNA expression of enzymes in the middle and last stages of glycolysis and alcoholic fermentation increased, and that of mitochondrial respiration enzymes decreased under short-term anaerobic conditions. Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) administration showed similar results under anaerobic conditions. Moreover, PFK1 maintained its regulatory effect under different conditions; no significant change was observed in its mRNA expression. Our results suggest that <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> obtains energy via carbohydrate catabolism in the early phase of environmental change and survives in various parts of the host.<\/jats:p>","DOI":"10.1371\/journal.pone.0284353","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T17:25:48Z","timestamp":1681925148000},"page":"e0284353","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Changes in the mRNA expression of glycolysis-related enzymes of Candida albicans during inhibition of intramitochondrial catabolism under anaerobic 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