{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T22:33:28Z","timestamp":1780353208037,"version":"3.54.1"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T00:00:00Z","timestamp":1683158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Introduction<\/jats:title>\n<jats:p>Chronic lower limb ulcers (CLLU) are those injuries that persist for more than six weeks despite adequate care. They are relatively common; it is estimated that 10\/1,000 people will develop CLLU in their lifetime. Diabetic ulcer, because of its unique pathophysiology (association between neuropathy, microangiopathy, and immune deficiency), is considered one of the most complex and difficult etiologies of CLLU for treatment. This treatment is complex, costly, and sometimes frustrating, as it is often ineffective, which worsens the quality of life of patients and makes its treatment a challenge.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Objective<\/jats:title>\n<jats:p>To describe a new method for treating diabetic CLLU and the initial results of using a new autologous tissue regeneration matrix.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Method<\/jats:title>\n<jats:p>This is a pilot, prospective, an interventional study that used a novel protocol of autologous tissue regeneration matrix for the treatment of diabetic CLLU.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Results<\/jats:title>\n<jats:p>Three male cases with a mean age of 54 years were included. A total of six Giant Pro PRF Membrane (GMPro) were used varying their application between one to three sessions during treatment. A total of 11 liquid phase infiltrations were performed varying their application between three and four sessions. The patients were evaluated weekly and a reduction in the wound area and scar retraction was observed during the period studied.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec005\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>The new tissue regeneration matrix described is an effective and low-cost method for the treatment of chronic diabetic ulcers.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0284701","type":"journal-article","created":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T17:33:35Z","timestamp":1683221615000},"page":"e0284701","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Progressive Platelet Rich Fibrin tissue regeneration matrix: Description of a novel, low cost and effective method for the treatment of chronic diabetic ulcers\u2014Pilot study"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5101-8206","authenticated-orcid":true,"given":"Carlos Jos\u00e9","family":"Saboia-Dantas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Dechichi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto Lichtsztejn","family":"Fech","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Vilhena","family":"de Carvalho Furst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3043-0728","authenticated-orcid":true,"given":"Rodrigo Daminello","family":"Raimundo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Antonio","family":"Correa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,5,4]]},"reference":[{"key":"pone.0284701.ref001","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.jconrel.2020.09.039","article-title":"Chronic wounds: Current status, available strategies and emerging therapeutic solutions","volume":"328","author":"K Las Heras","year":"2020","journal-title":"J Control Release"},{"issue":"6","key":"pone.0284701.ref002","doi-asserted-by":"crossref","first-page":"607","DOI":"10.3390\/cells8060607","article-title":"Regeneration of Dermis: Scarring and Cells Involved","volume":"8","author":"AL Rippa","year":"2019","journal-title":"Cells"},{"issue":"4","key":"pone.0284701.ref003","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s12192-008-0059-8","article-title":"Wound healing from a cellular stress response perspective","volume":"13","author":"BM Doshi","year":"2008","journal-title":"Cell Stress Chaperones"},{"issue":"1","key":"pone.0284701.ref004","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1152\/physrev.00067.2017","article-title":"Wound Healing: A Cellular Perspective","volume":"99","author":"M Rodrigues","year":"2019","journal-title":"Physiol Rev"},{"issue":"4","key":"pone.0284701.ref005","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.cjtee.2017.06.001","article-title":"The molecular biology in wound healing & non-healing wound","volume":"20","author":"C. 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