{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:18:59Z","timestamp":1747196339012,"version":"3.40.5"},"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T00:00:00Z","timestamp":1682467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This work was supported by King Saud University through the Vice Deanship of Scientific Research Chair and Glaucoma Research Chair in Ophthalmology.","award":["GRC-2022"],"award-info":[{"award-number":["GRC-2022"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"\nAim<\/jats:title>\nIn a retrospective and exploratory case-control study, we examined the genetic association of two common polymorphisms in the 3\u2019 untranslated region (UTR) of DICER1<\/jats:italic> (rs3742330) and DROSHA<\/jats:italic> (rs10719) genes in primary open-angle glaucoma (POAG) and primary angle-closure glaucoma (PACG), and its related clinical phenotypes in a Saudi cohort.<\/jats:p>\n<\/jats:sec>\n\nMethods<\/jats:title>\nDNA genotyping was performed using TaqMan real-time PCR assays in 500 participants, including 152 POAG, 102 PACG, and 246 non-glaucomatous controls. Statistical analyses were performed to examine the association(s).<\/jats:p>\n<\/jats:sec>\n\nResults<\/jats:title>\nAllele and genotype frequency of rs3742330 and rs10719 did not vary significantly in POAG and PACG compared to controls. No significant deviation was observed from Hardy-Weinberg Equilibrium (p<\/jats:italic> > 0.05). Gender stratification revealed no significant allelic\/genotype association with glaucoma types. Also, these polymorphisms showed no significant genotype effect on clinical markers such as intraocular pressure, cup\/disc ratio, and the number of antiglaucoma medications. Logistic regression showed no effect of age, sex, rs3742330, and rs10719 genotypes on the risk of disease outcome. We also examined a combined allelic effect of rs3742330 (A>G) and rs10719 (A>G). However, none of the allelic combinations significantly affected POAG and PACG.<\/jats:p>\n<\/jats:sec>\n\nConclusions<\/jats:title>\nThe 3\u2019 UTR polymorphisms rs3742330 and rs10719 of DICER1<\/jats:italic> and DROSHA<\/jats:italic> genes are not associated with POAG and PACG or its related glaucoma indices in this Middle-Eastern cohort of Saudi Arab ethnicity. However, there is a need to validate the results on a broader population and other ethnicities.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0284852","type":"journal-article","created":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T17:39:03Z","timestamp":1682530743000},"page":"e0284852","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["The 3\u2019 UTR polymorphisms rs3742330 in DICER1 and rs10719 in DROSHA genes are not associated with primary open-angle and angle-closure glaucoma: As case-control study"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5516-6951","authenticated-orcid":true,"given":"Altaf A.","family":"Kondkar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taif 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