{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T19:36:19Z","timestamp":1788896179521,"version":"build-2803163510"},"reference-count":77,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Arthrofibrosis following total knee arthroplasty is a fibroproliferative joint disorder marked by dysregulated biosynthesis of extracellular matrix proteins, such as collagens and proteoglycans. The underlying cellular events remain incompletely understood. Myofibroblasts are highly contractile matrix-producing cells characterized by increased alpha-smooth muscle actin expression and xylosyltransferase-I (XT-I) secretion. Human XT-I has been identified as a key mediator of arthrofibrotic remodeling. Primary fibroblasts from patients with arthrofibrosis provide a useful <jats:italic>in vitro<\/jats:italic> model to identify and characterize disease regulators and potential therapeutic targets. This study aims at characterizing primary synovial fibroblasts from arthrofibrotic tissues (AFib) regarding their molecular and cellular phenotype by utilizing myofibroblast cell culture models. Compared to synovial control fibroblasts (CF), AFib are marked by enhanced cell contractility and a higher XT secretion rate, demonstrating an increased fibroblast-to-myofibroblast transition rate during arthrofibrosis. Histochemical assays and quantitative gene expression analysis confirmed higher collagen and proteoglycan expression and accumulation in AFib compared to CF. Furthermore, fibrosis-based gene expression profiling identified novel modifier genes in the context of arthrofibrosis remodeling. In summary, this study revealed a unique profibrotic phenotype in AFib that resembles some traits of other fibroproliferative diseases and can be used for the future development of therapeutic interventions.<\/jats:p>","DOI":"10.1371\/journal.pone.0286334","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T17:21:47Z","timestamp":1685121707000},"page":"e0286334","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Understanding of arthrofibrosis: New explorative insights into extracellular matrix remodeling of synovial fibroblasts"],"prefix":"10.1371","volume":"18","author":[{"given":"Thanh-Diep","family":"Ly","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meike","family":"Sambale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Kl\u00f6sener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Traut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bastian","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Hendig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Kuhn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelius","family":"Knabbe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2234-9196","authenticated-orcid":true,"given":"Isabel","family":"Faust-Hinse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,5,26]]},"reference":[{"key":"pone.0286334.ref001","doi-asserted-by":"crossref","first-page":"e19.00223","DOI":"10.2106\/JBJS.RVW.19.00223","article-title":"Clinical management of arthrofibrosis: State of the art and therapeutic outlook","volume":"8","author":"I.O. 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Invest"},{"key":"pone.0286334.ref020","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1378\/chest.06-2568","article-title":"Molecular targets in pulmonary fibrosis","volume":"132","author":"C.J. Scotton","year":"2007","journal-title":"Chest"},{"key":"pone.0286334.ref021","doi-asserted-by":"crossref","first-page":"d12","DOI":"10.2741\/A171","article-title":"Cytokines in acute and chronic inflammation","volume":"2","author":"T.M. Wright","year":"1997","journal-title":"Front. Biosci"},{"key":"pone.0286334.ref022","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1172\/JCI93557","article-title":"Extracellular matrix as a driver of progressive fibrosis","volume":"128","author":"J. Herrera","year":"2018","journal-title":"J. Clin. Invest"},{"key":"pone.0286334.ref023","doi-asserted-by":"crossref","first-page":"G807","DOI":"10.1152\/ajpgi.00447.2014","article-title":"Novel insights into the function and dynamics of extracellular matrix in liver fibrosis","volume":"308","author":"M.A. Karsdal","year":"2015","journal-title":"Am. J. Physiol.\u2014Gastrointest. Liver Physiol"},{"key":"pone.0286334.ref024","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.matbio.2018.02.004","article-title":"Molecular and tissue alterations of collagens in fibrosis","volume":"68\u201369","author":"S. Ricard-Blum","year":"2018","journal-title":"Matrix Biol"},{"key":"pone.0286334.ref025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.matbio.2018.04.009","article-title":"Decoding fibrosis: Mechanisms and translational aspects","volume":"68\u201369","author":"L. Schaefer","year":"2018","journal-title":"Matrix Biol"},{"key":"pone.0286334.ref026","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3390\/jcdd6040035","article-title":"The non-fibrillar side of fibrosis: Contribution of the basement membrane, proteoglycans, and glycoproteins to myocardial fibrosis","volume":"6","author":"M. Chute","year":"2019","journal-title":"J. Cardiovasc. Dev. Dis"},{"key":"pone.0286334.ref027","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1046\/j.1523-1747.1999.00590.x","article-title":"Serum xylosyltransferase: A new biochemical marker of the sclerotic process in systemic sclerosis","volume":"112","author":"C. G\u00f6tting","year":"1999","journal-title":"J. Invest. 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Prante","year":"2007","journal-title":"J. Biol. Chem"},{"key":"pone.0286334.ref031","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.bbrc.2021.02.092","article-title":"Cytokine-mediated induction of human xylosyltransferase-I in systemic sclerosis skin fibroblasts","volume":"549","author":"T.-D. Ly","year":"2021","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"pone.0286334.ref032","doi-asserted-by":"crossref","first-page":"17779","DOI":"10.1038\/s41598-018-36217-2","article-title":"MicroRNA-29b mediates fibrotic induction of human xylosyltransferase-I in human dermal fibroblasts via the Sp1 pathway","volume":"8","author":"L. Riedel","year":"2018","journal-title":"Sci. Rep"},{"key":"pone.0286334.ref033","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s41231-021-00084-3","article-title":"Intra-articular adenosine, lidocaine and magnesium (ALM) solution decreases postoperative joint fibrosis in an experimental knee implant model","volume":"6","author":"J.L. Morris","year":"2021","journal-title":"Transl. Med. Commun"},{"key":"pone.0286334.ref034","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s13018-020-1544-8","article-title":"Expression of xylosyltransferases I and II and their role in the pathogenesis of arthrofibrosis","volume":"15","author":"A. Bernstein","year":"2020","journal-title":"J. Orthop. Surg"},{"key":"pone.0286334.ref035","doi-asserted-by":"crossref","first-page":"609","DOI":"10.3390\/biom10040609","article-title":"Activin A-mediated regulation of XT-I in human skin fibroblasts","volume":"10","author":"T.-D. Ly","year":"2020","journal-title":"Biomolecules"},{"key":"pone.0286334.ref036","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.3390\/biom10101467","article-title":"Identification of putative non-substrate-based XT-I inhibitors by natural product library screening","volume":"10","author":"T.-D. Ly","year":"2020","journal-title":"Biomolecules"},{"key":"pone.0286334.ref037","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1016\/j.ajpath.2012.02.004","article-title":"Recent developments in myofibroblast biology","volume":"180","author":"B. Hinz","year":"2012","journal-title":"Am. J. Pathol"},{"key":"pone.0286334.ref038","doi-asserted-by":"crossref","first-page":"100","DOI":"10.5301\/jsrd.5000240","article-title":"The mighty fibroblast and its utility in scleroderma research","volume":"2","author":"S.M. Garrett","year":"2017","journal-title":"J. Scleroderma Relat. Disord"},{"key":"pone.0286334.ref039","doi-asserted-by":"crossref","first-page":"9448","DOI":"10.1073\/pnas.1201840109","article-title":"Myofibroblasts revert to an inactive phenotype during regression of liver fibrosis","volume":"109","author":"T. Kisseleva","year":"2012","journal-title":"Proc. Natl. Acad. Sci"},{"key":"pone.0286334.ref040","doi-asserted-by":"crossref","first-page":"S15","DOI":"10.1016\/j.jhep.2015.02.039","article-title":"Hepatic fibrosis: Concept to treatment","volume":"62","author":"C. Trautwein","year":"2015","journal-title":"J. Hepatol"},{"key":"pone.0286334.ref041","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"M.W. Pfaffl","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"pone.0286334.ref042","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.yexcr.2017.09.001","article-title":"Characterization of dermal myofibroblast differentiation in pseudoxanthoma elasticum","volume":"360","author":"I. Faust","year":"2017","journal-title":"Exp. Cell Res"},{"key":"pone.0286334.ref043","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH image to ImageJ: 25 years of image analysis","volume":"9","author":"C.A. Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"pone.0286334.ref044","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/s004180050447","article-title":"In situ measurement of collagen synthesis by human bone cells with a Sirius Red-based colorimetric microassay: Effects of transforming growth factor \u03922 and ascorbic acid 2-phosphate","volume":"112","author":"H. Tullberg-Reinert","year":"1999","journal-title":"Histochem. Cell Biol"},{"key":"pone.0286334.ref045","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1177\/9.5.572","article-title":"The action of histological fixatives on tissue lipids. Comparison of the action of several fixatives using paper chromatography","volume":"9","author":"F.J.M. Heslinga","year":"1961","journal-title":"J. Histochem. Cytochem"},{"key":"pone.0286334.ref046","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/BF00306130","article-title":"Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian blue in salt solutions","volume":"5","author":"J.E. Scott","year":"1965","journal-title":"Histochemie"},{"key":"pone.0286334.ref047","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1038\/ejhg.2013.222","article-title":"Homozygous missense and nonsense mutations in BMPR1B cause acromesomelic chondrodysplasia-type Grebe","volume":"22","author":"L.M. Graul-Neumann","year":"2014","journal-title":"Eur. J. Hum. Genet"},{"key":"pone.0286334.ref048","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1093\/molehr\/8.12.1079","article-title":"High xylosyltransferase activities in human follicular fluid and cultured granulosa-lutein cells","volume":"8","author":"C. G\u00f6tting","year":"2002","journal-title":"Mol. Hum. Reprod"},{"key":"pone.0286334.ref049","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/0003-2697(84)90119-2","article-title":"Silk\u2014A new substrate for UDP-d-xylose: proteoglycan core protein \u03b2-d-xylosyltransferase","volume":"137","author":"P. Campbell","year":"1984","journal-title":"Anal. Biochem"},{"key":"pone.0286334.ref050","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.bbrc.2019.12.120","article-title":"MicroRNA-145 mediates xylosyltransferase-I induction in myofibroblasts via suppression of transcription factor KLF4","volume":"523","author":"T.-D. Ly","year":"2020","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"pone.0286334.ref051","doi-asserted-by":"crossref","first-page":"27","DOI":"10.3390\/ph11010027","article-title":"Glycosaminoglycans and proteoglycans","volume":"11","author":"V. Pomin","year":"2018","journal-title":"Pharmaceuticals"},{"key":"pone.0286334.ref052","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.addr.2017.07.010","article-title":"Drug targeting to myofibroblasts: Implications for fibrosis and cancer","volume":"121","author":"S. Yazdani","year":"2017","journal-title":"Adv. Drug Deliv. Rev"},{"key":"pone.0286334.ref053","doi-asserted-by":"crossref","first-page":"8549","DOI":"10.1167\/iovs.11-7956","article-title":"Role of the IL-6 classic- and trans-signaling pathways in corneal sterile inflammation and wound healing","volume":"52","author":"N. Ebihara","year":"2011","journal-title":"Investig. Opthalmology Vis. Sci"},{"key":"pone.0286334.ref054","doi-asserted-by":"crossref","first-page":"e0262549","DOI":"10.1371\/journal.pone.0262549","article-title":"Irradiated fibroblasts increase interleukin-6 expression and induce migration of head and neck squamous cell carcinoma","volume":"17","author":"S. Suzuki","year":"2022","journal-title":"PLOS One"},{"key":"pone.0286334.ref055","doi-asserted-by":"crossref","first-page":"20741","DOI":"10.18632\/oncotarget.15119","article-title":"IL-6 Secreted by cancer-associated fibroblasts promotes epithelial-mesenchymal transition and metastasis of gastric cancer via JAK2\/STAT3 signaling pathway","volume":"8","author":"X. Wu","year":"2017","journal-title":"Oncotarget"},{"key":"pone.0286334.ref056","doi-asserted-by":"crossref","first-page":"2703","DOI":"10.1016\/j.ygeno.2020.03.004","article-title":"Molecular pathology of human knee arthrofibrosis defined by RNA sequencing","volume":"112","author":"B. Bayram","year":"2020","journal-title":"Genomics"},{"key":"pone.0286334.ref057","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1080\/03008207.2018.1517759","article-title":"Arthrofibrosis and large joint scarring","volume":"60","author":"A.F. Chen","year":"2019","journal-title":"Connect. Tissue Res"},{"key":"pone.0286334.ref058","doi-asserted-by":"crossref","first-page":"2730","DOI":"10.1091\/mbc.12.9.2730","article-title":"Alpha-smooth muscle actin expression upregulates fibroblast contractile activity","volume":"12","author":"B. Hinz","year":"2001","journal-title":"Mol. Biol. Cell"},{"key":"pone.0286334.ref059","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.yjmcc.2018.07.130","article-title":"CTGF\/CCN2 is an autocrine regulator of cardiac fibrosis","volume":"121","author":"L.E. Dorn","year":"2018","journal-title":"J. Mol. Cell. Cardiol"},{"key":"pone.0286334.ref060","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1002\/glia.20537","article-title":"Transcriptional regulation of scar gene expression in primary astrocytes","volume":"55","author":"P. Gris","year":"2007","journal-title":"Glia"},{"key":"pone.0286334.ref061","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.gene.2017.01.025","article-title":"Molecular landscape of arthrofibrosis: Microarray and bioinformatic analysis of the temporal expression of 380 genes during contracture genesis","volume":"610","author":"M.E. Morrey","year":"2017","journal-title":"Gene"},{"key":"pone.0286334.ref062","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s15002-018-1371-z","article-title":"Prim\u00e4re Arthrofibrose nach Knie-Endoprothetik","volume":"21","author":"P. Traut","year":"2018","journal-title":"Orthop. Rheuma"},{"key":"pone.0286334.ref063","first-page":"1398","article-title":"Altered dermatan sulfate proteoglycan synthesis in fibroblast cultures established from skin of patients with systemic sclerosis","volume":"23","author":"G. Westergren-Thorsson","year":"1996","journal-title":"J. Rheumatol"},{"key":"pone.0286334.ref064","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1302\/2046-3758.33.2000276","article-title":"Intra-articular decorin influences the fibrosis genetic expression profile in a rabbit model of joint contracture","volume":"3","author":"M.P. Abdel","year":"2014","journal-title":"Bone Joint Res"},{"key":"pone.0286334.ref065","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1159\/000111805","article-title":"Diversity of fibroblasts\u2014a review on implications for skin tissue engineering","volume":"187","author":"S.V. 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Care Med"},{"key":"pone.0286334.ref072","doi-asserted-by":"crossref","first-page":"4992","DOI":"10.2741\/3583","article-title":"BMP-7 as antagonist of organ fibrosis","volume":"14","author":"R. Weiskirchen","year":"2009","journal-title":"Front. Biosci"},{"key":"pone.0286334.ref073","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.2741\/4189","article-title":"BMP-7 Counteracting TGF-beta1 activities in organ fibrosis","volume":"18","author":"R. Weiskirchen","year":"2013","journal-title":"Front. Biosci"},{"key":"pone.0286334.ref074","doi-asserted-by":"crossref","first-page":"e00360","DOI":"10.1042\/BSR20160107","article-title":"Involvement of matrix metalloproteinases (MMPs) and inflammasome pathway in molecular mechanisms of fibrosis","volume":"36","author":"S. Robert","year":"2016","journal-title":"Biosci. Rep"},{"key":"pone.0286334.ref075","doi-asserted-by":"crossref","first-page":"jcs239434","DOI":"10.1242\/jcs.239434","article-title":"\u0391v\u03b28 integrin adhesion and signaling pathways in development, physiology and disease","volume":"133","author":"J.H. McCarty","year":"2020","journal-title":"J. Cell Sci"},{"key":"pone.0286334.ref076","doi-asserted-by":"crossref","first-page":"2863","DOI":"10.1172\/JCI45589","article-title":"Mouse and human lung fibroblasts regulate dendritic cell trafficking, airway inflammation, and fibrosis through integrin \u0391v\u03b28\u2013mediated activation of TGF-\u03b2","volume":"121","author":"H. Kitamura","year":"2011","journal-title":"J. Clin. Invest"},{"key":"pone.0286334.ref077","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jaci.2005.08.057","article-title":"The CC chemokine eotaxin\/CCL11 has a selective profibrogenic effect on human lung fibroblasts","volume":"117","author":"I. Puxeddu","year":"2006","journal-title":"J. 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