{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T12:43:53Z","timestamp":1777898633903,"version":"3.51.4"},"reference-count":12,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T00:00:00Z","timestamp":1686614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI139052"],"award-info":[{"award-number":["R01AI139052"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["2R01AI130060"],"award-info":[{"award-number":["2R01AI130060"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI117211"],"award-info":[{"award-number":["R01AI117211"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Recently, we reported rifabutin hyper-activity against <jats:italic>Acinetobacter baumannii<\/jats:italic>. We sought to characterize if any additional rifamycins (n = 22) would also display hyper-activity when tested in iron-limited media against <jats:italic>A<\/jats:italic>. <jats:italic>baumannii<\/jats:italic>, <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic>, and <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>. MICs were determined against representative clinical isolates using the iron-limited media RPMI-1640. Only rifabutin was hyperactive against <jats:italic>A<\/jats:italic>. <jats:italic>baumannii<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pone.0287102","type":"journal-article","created":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T17:28:35Z","timestamp":1686677315000},"page":"e0287102","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Defining the minimum inhibitory concentration of 22 rifamycins in iron limited, physiologic medium against Acinetobacter baumannii, Escherichia coli, and Klebsiella pneumoniae clinical isolates"],"prefix":"10.1371","volume":"18","author":[{"given":"Peggy","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Alletto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bosul","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Burk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasmine","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Prati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7248-9453","authenticated-orcid":true,"given":"Emilia","family":"Caselli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brad","family":"Spellberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6718-4551","authenticated-orcid":true,"given":"Brian","family":"Luna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,6,13]]},"reference":[{"key":"pone.0287102.ref001","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1038\/s41564-020-0737-6","article-title":"A nutrient-limited screen unmasks rifabutin hyperactivity for extensively drug-resistant Acinetobacter baumannii","volume":"5","author":"B Luna","year":"2020","journal-title":"Nat Microbiol"},{"key":"pone.0287102.ref002","doi-asserted-by":"crossref","first-page":"e0092021","DOI":"10.1128\/msphere.00920-21","article-title":"In Vitro Activity of Rifabutin and Rifampin against Antibiotic-Resistant Acinetobacter baumannii, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae","volume":"6","author":"B Lee","year":"2021","journal-title":"mSphere"},{"key":"pone.0287102.ref003","first-page":"65","article-title":"Synergistic Rifabutin and Colistin Reduce Emergence of Resistance When Treating Acinetobacter baumannii","author":"J Cheng","year":"2021","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0287102.ref004","article-title":"In vitro activity of rifabutin against 293 contemporary carbapenem-resistant Acinetobacter baumannii clinical isolates and characterization of rifabutin mode of action and resistance mechanisms","author":"V Trebosc","year":"2020","journal-title":"J Antimicrob Chemother"},{"key":"pone.0287102.ref005","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing","year":"2022"},{"key":"pone.0287102.ref006","first-page":"e0132022","article-title":"Antimicrobial Susceptibility Testing Performed in RPMI 1640 Reveals Azithromycin Efficacy against Carbapenem-Resistant Acinetobacter baumannii and Predicts In Vivo Outcomes in Galleria mellonella","author":"S Miller","year":"2022","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0287102.ref007","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.7164\/antibiotics.40.1733","article-title":"CGP 4832, a new semisynthetic rifamycin derivative highly active against some gram-negative bacteria","volume":"40","author":"W Wehrli","year":"1987","journal-title":"J Antibiot"},{"key":"pone.0287102.ref008","first-page":"3505","article-title":"Highly efficient uptake of a rifamycin derivative via the FhuA-TonB-dependent uptake route in Escherichia coli","volume":"133","author":"AP Pugsley","year":"1987","journal-title":"J Gen Microbiol"},{"key":"pone.0287102.ref009","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/S0969-2126(01)00631-1","article-title":"Active transport of an antibiotic rifamycin derivative by the outer-membrane protein FhuA","volume":"9","author":"AD Ferguson","year":"2001","journal-title":"Structure"},{"key":"pone.0287102.ref010","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1054\/drup.1999.0107","article-title":"Active transport of siderophore-mimicking antibacterials across the outer membrane","volume":"2","author":"V. Braun","year":"1999","journal-title":"Drug Resist Updat"},{"key":"pone.0287102.ref011","article-title":"Adjunctive transferrin to reduce the emergence of antibiotic resistance in Gram-negative bacteria","author":"BM Luna","year":"2019","journal-title":"J Antimicrob Chemother"},{"key":"pone.0287102.ref012","first-page":"63","article-title":"Apotransferrin in Combination with Ciprofloxacin Slows Bacterial Replication, Prevents Resistance Amplification, and Increases Antimicrobial Regimen Effect","author":"PG Ambrose","year":"2019","journal-title":"Antimicrob Agents Chemother"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0287102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T17:28:48Z","timestamp":1686677328000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0287102"}},"subtitle":[],"editor":[{"given":"Yung-Fu","family":"Chang","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,6,13]]},"references-count":12,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0287102","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,13]]}}}