{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:26:01Z","timestamp":1789568761785,"version":"build-2803163510"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T00:00:00Z","timestamp":1713225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Health promotion \u201cResearch Center for Strategy of Health Promotion\u201d of Aomori University of health and welfare","award":["2020-5, 2021-1, 2024"],"award-info":[{"award-number":["2020-5, 2021-1, 2024"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>This study examined the psychogenic stress (PS) effects on changes in oxidative stress and the antioxidant capacity of an organism at different growth stages. The experimental animals were male Wistar rats of five different ages from growth periods (GPs) to old age. The growth stages were randomly classified into control (C) and experimental (PS) groups. The PS was performed using restraint and water immersion once daily for 3 h for 4 weeks. Reactive oxygen metabolites (d-ROMs) and the biological antioxidant potential (BAP) were measured before and after the experiment. In addition, the liver and adrenal glands were removed, and the wet weight was measured. The d-ROM and BAP of all growth stages given PS increased significantly. The d-ROM in the C group without PS increased significantly in GPs while decreased significantly in old-aged rats. In addition, the BAP of the C group in GP and early adulthood were all significantly elevated. There were significant differences in organ weights between the C and PS groups at all growth stages. Oxidative stress and antioxidant capacity differed depending on the organism\u2019s developmental status and growth stage, and PS also showed different effects. In particular, the variability in oxidative stress was remarkable, suggesting that the effect of PS was more significant in the organism\u2019s immature organs.<\/jats:p>","DOI":"10.1371\/journal.pone.0287421","type":"journal-article","created":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T17:37:46Z","timestamp":1713289066000},"page":"e0287421","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of psychogenic stress on oxidative stress and antioxidant capacity at different growth stages of rats: Experimental study"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4856-0264","authenticated-orcid":true,"given":"Sangun","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsunori","family":"Itagaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuko","family":"Satoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Issei","family":"Sugimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takumi","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiko","family":"Shibukawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruka","family":"Tatehana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,4,16]]},"reference":[{"key":"pone.0287421.ref001","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.1007\/s00213-019-05326-9","article-title":"Stress, sex hormones, inflammation, and major depressive disorder: Extending Social Signal Transduction Theory of Depression to account for sex differences in mood disorders","volume":"236","author":"GM Slavich","year":"2019","journal-title":"Psychopharmacology (Berl)"},{"key":"pone.0287421.ref002","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.yebeh.2014.03.023","article-title":"Contributions of fMRI towards our understanding of the response to psychosocial stress in epilepsy and psychogenic nonepileptic seizures","volume":"35","author":"JB Allendorfer","year":"2014","journal-title":"Epilepsy Behav"},{"key":"pone.0287421.ref003","first-page":"67","article-title":"The 2019 Novel Coronavirus Outbreak\u2014A Global Threat","volume":"68","author":"WY Khot","year":"2020","journal-title":"J Assoc Physicians India"},{"key":"pone.0287421.ref004","first-page":"e19212","volume":"13","author":"S Sharma","year":"2021","journal-title":"Impact of the COVID-19 Lockdown and Confinement Measures on the Musculoskeletal Health of the Urban Geriatric Population"},{"key":"pone.0287421.ref005","doi-asserted-by":"crossref","first-page":"159","DOI":"10.35772\/ghm.2022.01001","article-title":"Evaluating the daily life of child and adolescent psychiatric outpatients during temporary school closure over COVID-19 pandemic: A single-center case-control study in Japan","volume":"4","author":"Y Sasaki","year":"2022","journal-title":"Glob Health Med"},{"key":"pone.0287421.ref006","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.2147\/JIR.S275595","article-title":"Reactive Oxygen Species: Drivers of Physiological and Pathological Processes","volume":"13","author":"J Checa","year":"2020","journal-title":"J Inflamm Res"},{"key":"pone.0287421.ref007","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1080\/10253890.2019.1675627","article-title":"Acute restraint stress increases blood pressure and oxidative stress in the cardiorenal system of rats: a role for AT1 receptors","volume":"23","author":"GT do Vale","year":"2020","journal-title":"Stress"},{"key":"pone.0287421.ref008","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.clnesp.2019.02.003","article-title":"Effects of probiotics and synbiotic supplementation on antioxidant status: A meta-analysis of randomized clinical trials","volume":"30","author":"H Roshan","year":"2019","journal-title":"Clin Nutr ESPEN"},{"key":"pone.0287421.ref009","doi-asserted-by":"crossref","first-page":"669497","DOI":"10.3389\/fphys.2021.669497","article-title":"Metabolic Complications in Cardiac Aging","volume":"12","author":"T Sithara","year":"2021","journal-title":"Front physiol"},{"key":"pone.0287421.ref010","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1016\/j.freeradbiomed.2009.02.008","article-title":"Tissue-, substrate-, and site-specific characteristics of mitochondrial reactive oxygen species generation","volume":"46","author":"EB Tahara","year":"2009","journal-title":"Free Radic Biol Med"},{"key":"pone.0287421.ref011","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1080\/10715762.2017.1291939","article-title":"The effects of exercise load during development on oxidative stress levels and antioxidant potential in adulthood","volume":"51","author":"S Lee","year":"2017","journal-title":"Free Radic Res"},{"key":"pone.0287421.ref012","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1515\/jbcpp-2016-0038","article-title":"Repeated Restraint Stress Upregulates Rat Sulfotransferase 1A1","volume":"30","author":"R Balyan","year":"2018","journal-title":"J Basic Clin Physiol Pharmacol"},{"key":"pone.0287421.ref013","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.psyneuen.2018.10.019","article-title":"Stress and immunosenescence: The role of telomerase","volume":"101","author":"K de Punder","year":"2019","journal-title":"Psychoneuroendocrinology"},{"key":"pone.0287421.ref014","doi-asserted-by":"crossref","first-page":"130","DOI":"10.32471\/exp-oncology.2312-8852.vol-41-no-2.13093","article-title":"Prepuberal stress and obesity: effects on serum corticosterone, prolactin, testosterone and precancerous prostate lesions in adult rats","volume":"41","author":"D Herrera-Covarrubias","year":"2019","journal-title":"Exp Oncol"},{"key":"pone.0287421.ref015","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1055\/a-0970-9064","article-title":"Immune and Hormonal Response to High-intensity Exercise During Orienteering","volume":"40","author":"NC Araujo","year":"2019","journal-title":"Int J Sports Med"},{"key":"pone.0287421.ref016","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0736-5748(98)00034-3","article-title":"Responses to maternal separation: mechanisms and mediators","volume":"16","author":"CM Kuhn","year":"1998","journal-title":"Int J Dev Neurosci"},{"key":"pone.0287421.ref017","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1080\/13880209.2019.1682620","article-title":"Gastroprotective effects of Kangfuxin against water-immersion and restraint stress-induced gastric ulcer in rats: Roles of antioxidation, anti-inflammation, and pro-survival","volume":"57","author":"S Lu","year":"2019","journal-title":"Pharm. Boil"},{"key":"pone.0287421.ref018","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1179\/135100007X200218","article-title":"Development of oxidative stress and cell damage in the liver of rats with water-immersion restraint stress","volume":"12","author":"Y Ohta","year":"2007","journal-title":"Redox Rep"},{"key":"pone.0287421.ref019","doi-asserted-by":"crossref","first-page":"136","DOI":"10.3164\/jcbn.11-14","article-title":"Disruption of non-enzymatic antioxidant defense systems in the brain of rats with water-immersion restraint stress","volume":"51","author":"Y Ohta","year":"2012","journal-title":"J Clin Biochem Nutr"},{"key":"pone.0287421.ref020","doi-asserted-by":"crossref","first-page":"883","DOI":"10.3109\/10715762.2014.919390","article-title":"Assessment of oxidative stress in serum by d-ROMs test","volume":"48","author":"K Kilk","year":"2014","journal-title":"Free Radic Res"},{"key":"pone.0287421.ref021","unstructured":"WISMERLL. A high speed and all-in-one measuring device for reactive oxygen species \/ free radical levels and antioxidant level. https:\/\/wismerlleng.com\/productsservices\/redox-libra\/, Oct.15,2022."},{"key":"pone.0287421.ref022","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.physbeh.2011.03.002","article-title":"Opposing Effects of Chronic Stress and Weight Restriction on Cardiovascular, Neuroendocrine and Metabolic Function","volume":"104","author":"JN Flak","year":"2011","journal-title":"Physiol Behav"},{"key":"pone.0287421.ref023","doi-asserted-by":"crossref","first-page":"E685","DOI":"10.1152\/ajpendo.00121.2011","article-title":"Decreased plasma ghrelin contributes to anorexia following novelty stress","volume":"301","author":"Y Saegusa","year":"2011","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"pone.0287421.ref024","first-page":"250","article-title":"Body Weight and Organ Weight in rats","volume":"12","author":"S Tadokoro","year":"1962","journal-title":"Kita kanto Igaku"},{"key":"pone.0287421.ref025","doi-asserted-by":"crossref","first-page":"159","DOI":"10.5551\/jat1973.11.1_159","article-title":"Experimental Hypertension Inducesed by Long-Term Psychogenic Stress and Vascular Changes in Rats","volume":"11","author":"I Sekine","year":"1983","journal-title":"The journal of Japan Atherosclerosis Society"},{"key":"pone.0287421.ref026","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.pathophys.2012.07.002","article-title":"The impact of psychogenic stressors on oxidative stress markers and patterns of CYP2E1 expression in mice liver","volume":"19","author":"O Maksymchuk","year":"2012","journal-title":"Pathophysiology"},{"key":"pone.0287421.ref027","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1007\/BF01940708","article-title":"Restraint stress induced changes in rat liver and serum metallothionein and in Zn metabolism","volume":"42","author":"J Hidalgo","year":"1986","journal-title":"Experientia"},{"key":"pone.0287421.ref028","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.2174\/1871530320666200604160614","article-title":"Are Oxidative Stress and Inflammation Mediators of Bone Loss Due to Estrogen Deficiency? A Review of Current Evidence","volume":"20","author":"NV Mohamad","year":"2020","journal-title":"Endocr Metab Immune Disord Drug Targets"},{"key":"pone.0287421.ref029","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1152\/jappl.2001.90.3.1031","article-title":"Time course of responses of human skeletal muscle to oxidative stress induced by nondamaging exercise","volume":"90","author":"M Khassaf","year":"2001","journal-title":"J Appl Physiol"},{"key":"pone.0287421.ref030","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/j.1432-1033.1978.tb12051.x","article-title":"Do mitochondria produce oxygen radicals in vivo?","volume":"82","author":"H Nohl","year":"1978","journal-title":"Eur J Biochem"},{"key":"pone.0287421.ref031","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1007\/s12033-007-0048-7","article-title":"Alterations in Antioxidant Enzymes During Aging in Humans","volume":"37","author":"SI Rizvi","year":"2007","journal-title":"Mol Biotechnol"},{"key":"pone.0287421.ref032","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1111\/j.1600-0838.2010.01252.x","article-title":"An update: salivary hormones and physical exercise","volume":"21","author":"R Gatti","year":"2011","journal-title":"Scand J Med Sci Sports"},{"key":"pone.0287421.ref033","first-page":"1","article-title":"Effect of brief and low-intensity stretch exercise on core temperature, stress, and mood","volume":"110","author":"T Nagamatsu","year":"2012","journal-title":"Bulletin of the physical fitness research institute"},{"key":"pone.0287421.ref034","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1620\/tjem.221.43","article-title":"Ergometry Induces Systemic Oxidative Stress in Healthy Human Subjects","volume":"221","author":"M Antoncic-Svetina","year":"2010","journal-title":"Tohoku J Exp Med"},{"key":"pone.0287421.ref035","first-page":"213","article-title":"1999 Curt P. Richter award. Glucocorticoids and the regulation of memory consolidation","volume":"25","author":"B. Roozendaal","year":"2000","journal-title":"Psychoneuroendocrinology"},{"key":"pone.0287421.ref036","doi-asserted-by":"crossref","first-page":"3462","DOI":"10.3390\/jcm9113462","article-title":"The Relationship between Suicide and Oxidative Stress in a Group of Psychiatric Inpatients","volume":"9","author":"T Koweszko","year":"2020","journal-title":"J Clin Med"},{"key":"pone.0287421.ref037","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1046\/j.1471-4159.2000.740785.x","article-title":"Chronic stress induces the expression of inducible nitric oxide synthase in rat brain cortex","volume":"74","author":"R Olivenza","year":"2000","journal-title":"J Neurochem"},{"key":"pone.0287421.ref038","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.psyneuen.2014.09.025","article-title":"Is depression associated with increased oxidative stress? A systematic review and meta-analysis","volume":"51","author":"CN Black","year":"2015","journal-title":"Psychoneuroendocrinology"},{"key":"pone.0287421.ref039","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1002\/oby.22263","article-title":"Effects of Chronic NAD Supplementation on Energy Metabolism and Diurnal Rhythm in Obese Mice","volume":"26","author":"E Roh","year":"2018","journal-title":"Obesity (Silver Spring)"},{"key":"pone.0287421.ref040","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1159\/000122707","article-title":"Effects of Chronic Stress on Pituitary Hormone Release Induced by Combined Hemi-Extirpation of the Thyroid, Adrenal and Ovary in Rats","volume":"24","author":"P Du Ruisseau","year":"1977","journal-title":"Neuroendocrinology"},{"key":"pone.0287421.ref041","first-page":"56","article-title":"Accidental thymic involution in the growing body under the effect of different types of stressors","volume":"130","author":"MIu Kapitonova","year":"2006","journal-title":"Morfologiia"},{"key":"pone.0287421.ref042","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1248\/yakushi.14-00180","article-title":"Effects of 15-day Chronic Stress on Behavior and Neurological Changes in the Hippocampus of ICR Mice","volume":"135","author":"Y Sakamoto","year":"2015","journal-title":"Yakugaku Zasshi"},{"key":"pone.0287421.ref043","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1002\/jcp.1040980219","article-title":"Superoxide dismutase specific activities in cultured human diploid cells of various donor ages","volume":"98","author":"MR Duncan","year":"1979","journal-title":"J Cell Physiol"},{"key":"pone.0287421.ref044","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1080\/10253890.2018.1507021","article-title":"Comparative evaluation of adolescent repeated psychological or physical stress effects on adult cognitive performance, oxidative stress, and heart rate in female rats","volume":"22","author":"MS Mousavi","year":"2019","journal-title":"Stress"},{"key":"pone.0287421.ref045","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1016\/j.exger.2007.03.008","article-title":"Prevention of oxidative damage that contributes to the loss of bioenergetic capacity in ageing skin","volume":"42","author":"H Corstjens","year":"2007","journal-title":"Exp Gerontol"},{"key":"pone.0287421.ref046","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1016\/S0891-5849(98)00025-2","article-title":"Alterations of antioxidant enzymes and oxidative damage to macromolecules in different organs of rats during aging","volume":"24","author":"L Tian","year":"1998","journal-title":"Free Radic Biol Med"},{"key":"pone.0287421.ref047","doi-asserted-by":"crossref","first-page":"3722","DOI":"10.1073\/pnas.1417566112","article-title":"Regulation of Nrf2 signaling and longevity in naturally long-lived rodents","volume":"112","author":"KN Lewis","year":"2015","journal-title":"Proc Natl Acad Sci USA"},{"key":"pone.0287421.ref048","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1016\/j.cell.2016.05.017","article-title":"Repression of the Antioxidant NRF2 Pathway in Premature Aging","volume":"165","author":"N Kubben","year":"2016","journal-title":"Cell"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0306774","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T00:00:00Z","timestamp":1720137600000}}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0287421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T21:56:25Z","timestamp":1713909385000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0287421"}},"subtitle":[],"editor":[{"given":"Yasmina","family":"Abd\u2010Elhakim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,4,16]]},"references-count":48,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,4,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0287421","relation":{"correction":[{"id-type":"doi","id":"10.1371\/journal.pone.0306774","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,16]]}}}