{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T08:12:26Z","timestamp":1769069546285,"version":"3.49.0"},"reference-count":99,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T00:00:00Z","timestamp":1698883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Necrotizing enterocolitis (NEC) is a severe intestinal disease of the newborn infants, associated with high morbidity and mortality. It has been reported that <jats:italic>Bifidobacterium<\/jats:italic> could protect the intestinal barrier function and reduce the risk of NEC. This study aimed to evaluate the probiotic potential of <jats:italic>Bifidobacterium<\/jats:italic> strains isolated from the chicken intestines and its effect on necrotizing enterocolitis in newborn SD rats. Out of 32 isolates, <jats:italic>B<\/jats:italic>. <jats:italic>breve<\/jats:italic> AHC3 not only exhibited excellent probiotic potential, including tolerance to artificial simulated gastric conditions, adhesion to HT-29 cells, antioxidant capacity and antibacterial activity, but also possessed reliable safety. Additionally, NEC model was established to further investigate the effect of <jats:italic>B<\/jats:italic>. <jats:italic>breve<\/jats:italic> AHC3 on necrotizing enterocolitis in newborn SD rats. It was illustrated that administration of <jats:italic>B<\/jats:italic>. <jats:italic>breve<\/jats:italic> AHC3 significantly not only reduced the incidence of NEC (from 81.25% to 34.38%) (<jats:italic>P<\/jats:italic>&lt; 0.05), but also alleviated the severity of ileal injury (<jats:italic>P<\/jats:italic>&lt; 0.05). Compared with NEC model, <jats:italic>B<\/jats:italic>. <jats:italic>breve<\/jats:italic> AHC3 could significantly decrease the level of proinflammatory factor TNF-\u03b1 (<jats:italic>P<\/jats:italic>&lt; 0.05) and increase the level of antiinflammatory factor IL-10 (<jats:italic>P<\/jats:italic>&lt; 0.05) in the ileum of NEC rats. Through the intervention of <jats:italic>B<\/jats:italic>. <jats:italic>breve<\/jats:italic> AHC3, the gray value of inducible nitric oxide synthase (iNOS) in intestinal tissue of NEC rats was significantly reduced (<jats:italic>P<\/jats:italic>&lt; 0.05). It was indicated that <jats:italic>B<\/jats:italic>. <jats:italic>breve<\/jats:italic> AHC3 exhibited prominent probiotic potential and reliable safety. In the neonatal SD rat model of NEC, <jats:italic>B<\/jats:italic>. <jats:italic>breve<\/jats:italic> AHC3 had an available protective effect on the intestinal injury of NEC, which might be related to reducing the inflammatory reaction in the ileum and inhibiting the expression of iNOS in intestinal tissue cells. <jats:italic>B<\/jats:italic>. <jats:italic>breve<\/jats:italic> AHC3 could be used as a potential treatment for human NEC.<\/jats:p>","DOI":"10.1371\/journal.pone.0287799","type":"journal-article","created":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T18:03:32Z","timestamp":1698948212000},"page":"e0287799","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification and evaluation of probiotic potential of Bifidobacterium breve AHC3 isolated from chicken intestines and its effect on necrotizing enterocolitis (NEC) in newborn SD rats"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0627-9212","authenticated-orcid":true,"given":"Xiaopei","family":"Lin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changjun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,11,2]]},"reference":[{"issue":"5","key":"pone.0287799.ref001","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.3906\/sag-2103-275","article-title":"The protective effects of Anakinra in a neonatal rat model of necrotizing enterocolitis.","volume":"51","author":"H G\u00fcne\u015f","year":"2021","journal-title":"Turk J Med Sci"},{"issue":"6","key":"pone.0287799.ref002","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1080\/14767058.2018.1506759","article-title":"The protective effects of sesamol in a neonatal rat model of necrotizing 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