{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:38:32Z","timestamp":1775198312199,"version":"3.50.1"},"reference-count":88,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:00:00Z","timestamp":1687996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000410","name":"Beit Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Brucella<\/jats:italic> spp., <jats:italic>Toxoplasma gondii<\/jats:italic>, and <jats:italic>Chlamydia abortus<\/jats:italic> have long been recognized as zoonoses and significant causes of reproductive failure in small ruminants globally. A cross-sectional study was conducted in August 2020 to determine the seroprevalences of <jats:italic>Brucella<\/jats:italic> spp., <jats:italic>Toxoplasma gondii<\/jats:italic> and <jats:italic>Chlamydia abortus<\/jats:italic> in 398 small ruminants from four districts of Zimbabwe (Chivi, Makoni, Zvimba, and Goromonzi) using Indirect-ELISAs. A structured questionnaire was used to assess the knowledge, attitudes, and practices of 103 smallholder farmers towards small ruminant abortions, <jats:italic>Brucella<\/jats:italic> spp., <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic> and <jats:italic>C<\/jats:italic>. <jats:italic>abortus<\/jats:italic>, and to obtain a general overview of the significance of small ruminant reproductive failure(s) on their livelihoods. The overall seroprevalences were: 9.1% (95% CI: 6.4\u201312.3) for <jats:italic>Brucella<\/jats:italic> spp., 6.8% (95% CI: 4.5\u20139.7) for <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic> and 2.0% (95% CI: 0.9\u20133.9) for <jats:italic>C<\/jats:italic>. <jats:italic>abortus<\/jats:italic>. Location, age, parity, and abortion history were associated with <jats:italic>Brucella<\/jats:italic> spp. seropositivity. Location was also associated with both <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic> and <jats:italic>C<\/jats:italic>. <jats:italic>abortus<\/jats:italic> seropositivity. The questionnaire survey established that 44% of respondents had recently faced reproductive disease challenges within their flocks, with 34% correctly identifying abortion causes and only 10%, 6% and 4% having specific knowledge of <jats:italic>Brucella<\/jats:italic> spp., <jats:italic>C<\/jats:italic>. <jats:italic>abortus<\/jats:italic> and <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic>, respectively. This study provides the first serological evidence of <jats:italic>Brucella<\/jats:italic> spp. in small ruminants since 1996 and builds the evidence on small ruminant toxoplasmosis and chlamydiosis in Zimbabwe. Evidence of these zoonoses in small ruminants and the paucity of knowledge shows the need for a coordinated One Health approach to increase public awareness of these diseases, and to establish effective surveillance and control measures. Further studies are required to establish the role these diseases play in small ruminant reproductive failure(s), to identify the <jats:italic>Brucella<\/jats:italic> spp. detected here to species\/subspecies level, and to assess the socio-economic impact of reproductive failure in livestock among marginalised rural communities.<\/jats:p>","DOI":"10.1371\/journal.pone.0287902","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T18:17:30Z","timestamp":1688062650000},"page":"e0287902","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Seroprevalence and assessment of public awareness of Brucella spp., Toxoplasma gondii and Chlamydia abortus in small ruminants from selected smallholder commercial farms of Zimbabwe"],"prefix":"10.1371","volume":"18","author":[{"given":"Dey 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Homann","year":"2007"},{"key":"pone.0287902.ref003","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.smallrumres.2018.05.010","article-title":"Goat flock productivity under smallholder farmer management in Zimbabwe.","volume":"164","author":"TT Mhlanga","year":"2018","journal-title":"Small Ruminant Research.1"},{"issue":"3","key":"pone.0287902.ref004","first-page":"91","article-title":"Breeding of goats: An indigenous approach to enhancing opportunities for smallholder farmers in Inyathi, Zimbabwe.","volume":"11","author":"C Ndlovu","year":"2020","journal-title":"IJLP."},{"issue":"4","key":"pone.0287902.ref005","doi-asserted-by":"crossref","first-page":"267","DOI":"10.4102\/ojvr.v72i4.175","article-title":"Seroprevalence of Toxoplasma gondii infection in goats and sheep in Zimbabwe","volume":"72","author":"T Hove","year":"2005","journal-title":"Onderstepoort Journal of Veterinary Research"},{"key":"pone.0287902.ref006","first-page":"28","article-title":"An investigation into the cause of high abortion rates of goats in the Limpopo Province, South Africa.","volume":"28","author":"R Ndou","year":"2012","journal-title":"Continuing Education Presenters"},{"key":"pone.0287902.ref007","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.prevetmed.2017.08.004","article-title":"Seroprevalence of brucellosis in cattle and selected wildlife species at selected livestock\/wildlife interface areas of the Gonarezhou National Park, Zimbabwe.","volume":"146","author":"M Ndengu","year":"2017","journal-title":"Preventive Veterinary Medicine."},{"issue":"5","key":"pone.0287902.ref008","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1007\/s11250-011-9794-4","article-title":"Seroprevalence of brucellosis and its associated risk factors in cattle from smallholder dairy farms in Zimbabwe.","volume":"43","author":"G Matope","year":"2011","journal-title":"Trop Anim Health Prod."},{"key":"pone.0287902.ref009","article-title":"Infectious abortion and associated risk factors in dairy cattle farms in Nakuru district, Kenya","author":"TA Okumu","year":"2014"},{"issue":"3","key":"pone.0287902.ref010","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1016\/j.rvsc.2013.08.007","article-title":"Some risk factors for reproductive failures and contribution of Toxoplasma gondii infection in sheep and goats of Central Ethiopia: A cross-sectional study","volume":"95","author":"EZ Gebremedhin","year":"2013","journal-title":"Research in Veterinary Science"},{"issue":"1","key":"pone.0287902.ref011","first-page":"25","article-title":"A sero-survey of major infectious causes of abortion in small ruminants in Morocco.","volume":"51","author":"A Benkirane","year":"2015","journal-title":"Veterinaria Italiana"},{"issue":"5","key":"pone.0287902.ref012","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1007\/s11250-018-1536-4","article-title":"Sero-prevalence of chlamydiosis in cattle and selected wildlife species at a wildlife\/livestock interface area of Zimbabwe.","volume":"50","author":"M Ndengu","year":"2018","journal-title":"Trop Anim Health Prod."},{"key":"pone.0287902.ref013","first-page":"8","article-title":"FAO works to curb the burden of brucellosis in endemic countries: Case studies from Eurasia and the Near East","author":"A. Idrissi","year":"2014","journal-title":"FAO focus on."},{"key":"pone.0287902.ref014","article-title":"Isolation of Toxoplasma gondii from goats with a history of reproductive disorders and the prevalence of Toxoplasma and chlamydial antibodies.","author":"SP Sharma","year":"2003"},{"issue":"1","key":"pone.0287902.ref015","doi-asserted-by":"crossref","first-page":"62","DOI":"10.5604\/12321966.1141370","article-title":"Toxoplasma gondii in small ruminants in Northern Italy\u2013prevalence and risk factors.","volume":"22","author":"AL Gazzonis","year":"2015","journal-title":"Annals of Agricultural and Environmental Medicine"},{"issue":"1","key":"pone.0287902.ref016","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1186\/s12889-016-2744-3","article-title":"Living at the edge of an interface area in Zimbabwe: cattle owners, commodity chain and health workers\u2019 awareness, perceptions and practices on zoonoses.","volume":"16","author":"BM Gadaga","year":"2016","journal-title":"BMC Public Health.28"},{"issue":"7","key":"pone.0287902.ref017","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1017\/S0950268817000097","article-title":"Assessment of community awareness and risk perceptions of zoonotic causes of abortion in cattle at three selected livestock\u2013wildlife interface areas of Zimbabwe.","volume":"145","author":"M Ndengu","year":"2017","journal-title":"Epidemiology & Infection."},{"issue":"3","key":"pone.0287902.ref018","doi-asserted-by":"crossref","first-page":"116","DOI":"10.4102\/jsava.v78i3.301","article-title":"Seroprevalence of toxoplasmosis in sheep in South Africa","volume":"78","author":"N Abu Samraa","year":"2007","journal-title":"Journal of the South African Veterinary Association"},{"issue":"1","key":"pone.0287902.ref019","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4102\/ojvr.v86i1.1670","article-title":"Brucellosis and chlamydiosis seroprevalence in goats at livestock-wildlife interface areas of Zimbabwe","volume":"86","author":"S Bhandi","year":"2019","journal-title":"Onderstepoort Journal of Veterinary Research"},{"issue":"7","key":"pone.0287902.ref020","doi-asserted-by":"crossref","first-page":"e0008461","DOI":"10.1371\/journal.pntd.0008461","article-title":"Brucellosis in ruminants and pastoralists in Borena, Southern Ethiopia.","volume":"14","author":"BM Edao","year":"2020","journal-title":"PLOS Neglected Tropical Diseases."},{"key":"pone.0287902.ref021","doi-asserted-by":"crossref","first-page":"e7239156","DOI":"10.1155\/2018\/7239156","article-title":"Comparative Seroepidemiological Study of Brucellosis in Sheep under Smallholder Farming and Governmental Breeding Ranches of Central and North East Ethiopia","volume":"2018","author":"SA Addis","year":"2018","journal-title":"Journal of Veterinary Medicine"},{"issue":"14","key":"pone.0287902.ref022","first-page":"140","article-title":"Smallholder Agriculture Production in Zimbabwe: A Survey","author":"C. Mutami","year":"2015","journal-title":"Consilience."},{"key":"pone.0287902.ref023","article-title":"Zimbabwe: Agro-ecological Zones Map [with administrative boundaries] (as of 05 Oct 2009)\u2014Zimbabwe","author":"OCHA","year":"2009","journal-title":"ReliefWeb"},{"key":"pone.0287902.ref024","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1002\/9781118280249","volume-title":"Veterinary Epidemiology","author":"M Thrusfield","year":"2018","edition":"4"},{"issue":"1","key":"pone.0287902.ref025","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.vetmic.2015.07.010","article-title":"Chlamydiaceae and chlamydial infections in sheep or goats","volume":"181","author":"A Rodolakis","year":"2015","journal-title":"Veterinary Microbiology"},{"issue":"3","key":"pone.0287902.ref026","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0167-5877(00)00108-2","article-title":"Risk factors for brucellosis seroprevalence of sheep and goat flocks in Spain.","volume":"44","author":"FJ Reviriego","year":"2000","journal-title":"Preventive Veterinary Medicine."},{"issue":"4","key":"pone.0287902.ref027","doi-asserted-by":"crossref","first-page":"65","DOI":"10.3390\/vetsci4040065","article-title":"Risk Factors Associated with Brucella Seropositivity in Sheep and Goats in Duhok Province, Iraq.","volume":"4","author":"AG Alhamada","year":"2017","journal-title":"Veterinary Sciences."},{"issue":"1","key":"pone.0287902.ref028","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0167-5877(00)00116-1","article-title":"Application of diagnostic tests in veterinary epidemiologic studies.","volume":"45","author":"M Greiner","year":"2000","journal-title":"Preventive Veterinary Medicine."},{"key":"pone.0287902.ref029","first-page":"1456","article-title":"Chapter 3.8.5: Enzootic abortion of ewes (Ovine Chlamydiosis) (Infection with Chlamydia abortus).","author":"WOAH","year":"2018","journal-title":"Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, World Organisation for Animal Health"},{"issue":"10","key":"pone.0287902.ref030","doi-asserted-by":"crossref","first-page":"587","DOI":"10.3923\/ajava.2016.587.593","article-title":"Chlamydophila abortus Infection in Small Ruminants: A Review","volume":"11","author":"A. Selim","year":"2016","journal-title":"Asian J of Animal and Veterinary Advances."},{"issue":"10","key":"pone.0287902.ref031","first-page":"913","article-title":"Specificity dependence between serological tests for diagnosing bovine brucellosis in Brucella-free farms showing false positive serological reactions due to Yersinia enterocolitica O:9.","volume":"46","author":"RC Mainar-Jaime","year":"2005","journal-title":"Can Vet J."},{"issue":"4","key":"pone.0287902.ref032","doi-asserted-by":"crossref","first-page":"205","DOI":"10.4102\/jsava.v82i4.75","article-title":"Brucellae through the food chain: the role of sheep, goats and springbok (Antidorcus marsupialis) as sources of human infections in Namibia.","volume":"82","author":"K Magwedere","year":"2011","journal-title":"Journal of the South African Veterinary Association"},{"key":"pone.0287902.ref033","first-page":"103","volume-title":"Brucellosis in Humans and Animals","author":"MJ Corbel","year":"2006"},{"issue":"1","key":"pone.0287902.ref034","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1080\/09712119.2016.1222942","article-title":"Comparative evaluation of three serological tests for the detection of Brucella antibodies from infected cattle herds","volume":"45","author":"S Erdenli\u011f G\u00fcrbilek","year":"2017","journal-title":"Journal of Applied Animal Research"},{"issue":"4","key":"pone.0287902.ref035","doi-asserted-by":"crossref","first-page":"296","DOI":"10.3325\/cmj.2010.51.296","article-title":"Diagnosis of Brucellosis in Livestock and Wildlife.","volume":"51","author":"J Godfroid","year":"2010","journal-title":"Croat Med J."},{"issue":"1","key":"pone.0287902.ref036","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0378-1135(02)00229-8","article-title":"Diagnosis of brucellosis by serology","volume":"90","author":"K. Nielsen","year":"2002","journal-title":"Veterinary Microbiology"},{"issue":"5","key":"pone.0287902.ref037","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1017\/S0950268811001178","article-title":"Seroepidemiological study of livestock brucellosis in a pastoral region.","volume":"140","author":"B Megersa","year":"2012","journal-title":"Epidemiology & Infection."},{"issue":"5","key":"pone.0287902.ref038","doi-asserted-by":"crossref","first-page":"e0007311","DOI":"10.1371\/journal.pntd.0007311","article-title":"Molecular characterization of Brucella species from Zimbabwe.","volume":"13","author":"MB Ledwaba","year":"2019","journal-title":"PLOS Neglected Tropical Diseases."},{"key":"pone.0287902.ref039","first-page":"133","article-title":"Current state of brucellosis in Zimbabwe","author":"M. Madsen","year":"1989","journal-title":"Zimbabwe veterinary journal"},{"issue":"1","key":"pone.0287902.ref040","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1186\/1756-0500-2-261","article-title":"Characterization of some Brucella species from Zimbabwe by biochemical profiling and AMOS-PCR.","volume":"2","author":"G Matope","year":"2009","journal-title":"BMC Res Notes."},{"key":"pone.0287902.ref041","first-page":"1753","volume-title":"Manual of Diagnostic Tests and Vaccines for Terrestrial Animals","author":"WOAH","year":"2018"},{"issue":"8","key":"pone.0287902.ref042","doi-asserted-by":"crossref","first-page":"e0005692","DOI":"10.1371\/journal.pntd.0005692","article-title":"Caprine brucellosis: A historically neglected disease with significant impact on public health.","volume":"11","author":"CA Rossetti","year":"2017","journal-title":"PLOS Neglected Tropical Diseases."},{"key":"pone.0287902.ref043","first-page":"1419:1","article-title":"S\u00e9ropr\u00e9valence des maladies abortives zoonotiques chez les ruminants au nord de la Tunisie.","volume":"2","author":"BR Elandalousi","year":"2015","journal-title":"Research ISSN: 2334-1009. 2015 Jun 2;Research fr"},{"issue":"4","key":"pone.0287902.ref044","first-page":"111","article-title":"Seroprevalence of small ruminant brucellosis and its effect on reproduction at Tellalak District of Afar region, Ethiopia.","volume":"7","author":"WM Tadeg","year":"2015","journal-title":"JVMAH."},{"key":"pone.0287902.ref045","article-title":"Small Ruminant Brucellosis and Public Health Awareness in Two Districts of Afar Region, Ethiopia","author":"A Tegegn","year":"2016","journal-title":"Journal of Veterinary Science and Technology"},{"issue":"1","key":"pone.0287902.ref046","doi-asserted-by":"crossref","first-page":"45","DOI":"10.4314\/acsj.v24i1.5S","article-title":"Prevalence of brucellosis in livestock and incidences in humans in east Africa","volume":"24","author":"AC Chota","year":"2016","journal-title":"African Crop Science Journal"},{"key":"pone.0287902.ref047","volume-title":"Seroprevalence of brucellosis in small ruminants and farmer awareness of the disease on selected farms in Beatrice, Zimbabwe [Dissertation].","author":"C Musarandoga","year":"2013"},{"issue":"1","key":"pone.0287902.ref048","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s11250-011-9890-5","article-title":"Survey of brucellosis at the wildlife\u2013livestock interface on the Zimbabwean side of the Great Limpopo Transfrontier Conservation Area.","volume":"44","author":"C Gomo","year":"2012","journal-title":"Trop Anim Health Prod."},{"issue":"3","key":"pone.0287902.ref049","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s11250-006-4320-9","article-title":"Prevalence of antibodies to Brucella spp. and individual risk Factors of Infection in Traditional Cattle, Goats and Sheep Reared in Livestock\u2013Wildlife Interface Areas of Zambia","volume":"38","author":"JB Muma","year":"2006","journal-title":"Trop Anim Health Prod."},{"key":"pone.0287902.ref050","volume-title":"SADCC Congress on Animal Diseases","author":"RW Halliwell","year":"1987"},{"key":"pone.0287902.ref051","volume-title":"Impact of a goat pass-on scheme on goat management and productivity in Chipinge district","author":"P. Musingarabwi","year":"2016"},{"key":"pone.0287902.ref052","article-title":"Govt launches Command Livestock","author":"Z. Murwira","year":"2017","journal-title":"The Herald."},{"key":"pone.0287902.ref053","article-title":"Heifer pass-on scheme rolled out in Manicaland, Masvingo","author":"R. Zinyuke","year":"2020","journal-title":"The Herald."},{"key":"pone.0287902.ref054","article-title":"Seroprevalence of brucellosis and risk factors associated with its seropositivity in cattle, goats and humans in Iganga District, Uganda.","author":"J Nguna","year":"2019","journal-title":"The Pan African Medical Journal"},{"key":"pone.0287902.ref055","article-title":"Brucellosis surveillance and control in Zimbabwe: bacteriological and serological investigation in dairy herds.","author":"K Mohan","year":"1996"},{"issue":"3","key":"pone.0287902.ref056","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1080\/00034983.1992.11812671","article-title":"The seroprevalence of toxoplasmosis in sheep, goats and pigs in Zimbabwe.","volume":"86","author":"VS Pandey","year":"1992","journal-title":"Annals of Tropical Medicine & Parasitology"},{"issue":"1","key":"pone.0287902.ref057","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1186\/1756-0500-7-696","article-title":"Seroprevalence and risk factors of Toxoplasma gondii infection in sheep and goats slaughtered for human consumption in Central Ethiopia.","volume":"7","author":"EZ Gebremedhin","year":"2014","journal-title":"BMC Res Notes."},{"issue":"3","key":"pone.0287902.ref058","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.vetpar.2012.07.020","article-title":"Toxoplasma gondii in sheep and goats: Seroprevalence and potential risk factors under dairy husbandry practices","volume":"190","author":"N Tzanidakis","year":"2012","journal-title":"Veterinary Parasitology"},{"issue":"1","key":"pone.0287902.ref059","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.vetpar.2007.07.003","article-title":"Toxoplasma gondii and ovine toxoplasmosis: New aspects of an old story","volume":"149","author":"D Buxton","year":"2007","journal-title":"Veterinary Parasitology"},{"issue":"1","key":"pone.0287902.ref060","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4102\/ojvr.v86i1.1688","article-title":"Seroprevalence and associated risk factors of Toxoplasma gondii infection in domestic animals in southeastern South Africa","volume":"86","author":"WM Tagwireyi","year":"2019","journal-title":"Onderstepoort Journal of Veterinary Research"},{"issue":"10","key":"pone.0287902.ref061","first-page":"486","article-title":"Serological survey on Toxoplasmosis in sheep and goats in Nazareth, Ethiopia.","volume":"155","author":"T Negash","year":"2004","journal-title":"Revue de medecine veterinaire."},{"issue":"2","key":"pone.0287902.ref062","first-page":"91","article-title":"Seroprevalence of Toxoplasmosis in Sheep in Southern Punjab, Pakistan.","volume":"30","author":"MH Lashari","year":"2010","journal-title":"Pak Vet J."},{"issue":"2","key":"pone.0287902.ref063","first-page":"113","article-title":"Seroprevalence of Toxoplasma gondii infection among sheep and goats in the Stara Zagora region","volume":"11","author":"P Prelezov","year":"2008","journal-title":"Bulgarian Journal of Veterinary Medicine"},{"issue":"3","key":"pone.0287902.ref064","doi-asserted-by":"crossref","first-page":"752","DOI":"10.3390\/su12030752","article-title":"Drought Risk to Agricultural Systems in Zimbabwe: A Spatial Analysis of Hazard, Exposure, and Vulnerability.","volume":"12","author":"J Frischen","year":"2020","journal-title":"Sustainability."},{"issue":"1","key":"pone.0287902.ref065","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12917-016-0906-2","article-title":"Seroepidemiology and associated risk factors of Toxoplasma gondii in sheep and goats in Southwestern Ethiopia","volume":"12","author":"D Tegegne","year":"2016","journal-title":"BMC Veterinary Research"},{"issue":"1","key":"pone.0287902.ref066","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4102\/ojvr.v82i1.993","article-title":"The prevalence of antibodies to Toxoplasma gondii in sheep in the Western Cape, South Africa: research communication.","volume":"82","author":"K Hammond-Aryee","year":"2015","journal-title":"Onderstepoort Journal of Veterinary Research"},{"issue":"3","key":"pone.0287902.ref067","first-page":"91","article-title":"Antibodies to Chlamydia psittaci in goats in Botswana.","volume":"29","author":"MG Binta","year":"1998","journal-title":"Zimbabwe Veterinary Journal"},{"issue":"6","key":"pone.0287902.ref068","first-page":"38","article-title":"An investigation into the prevalence of Toxoplasma gondii among indigenous, communally reared goats in the Mafikeng area of the North West Province of South Africa","volume":"8","author":"RV Ndou","year":"2011","journal-title":"Life Science Journal"},{"issue":"2","key":"pone.0287902.ref069","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1051\/parasite\/2007142155","article-title":"Serological survey of caprine toxoplasmosis in Ethiopia: Prevalence and risk factors.","volume":"14","author":"S Teshale","year":"2007","journal-title":"Parasite"},{"issue":"3","key":"pone.0287902.ref070","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/s12639-018-01076-1","article-title":"A serological survey of Toxoplasma gondii infection in sheep and goat from Benin, West-Africa.","volume":"43","author":"ABN Tonouhewa","year":"2019","journal-title":"J Parasit Dis."},{"issue":"3","key":"pone.0287902.ref071","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.vetpar.2014.08.003","article-title":"Spatial distribution of Toxoplasma gondii oocysts in soil in a rural area: Influence of cats and land use","volume":"205","author":"C Gotteland","year":"2014","journal-title":"Veterinary Parasitology"},{"issue":"1","key":"pone.0287902.ref072","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0001-706X(00)00084-X","article-title":"The prevalence of anti-Toxoplasma gondii antibodies in Ghanaian sheep and goats","volume":"76","author":"WNA van der Puije","year":"2000","journal-title":"Acta Tropica"},{"issue":"1","key":"pone.0287902.ref073","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1186\/s12917-020-02360-0","article-title":"Seroprevalence and associated risk factors for chlamydiosis, coxiellosis and brucellosis in sheep and goats in Borana pastoral area, southern","volume":"16","author":"A Tesfaye","year":"2020","journal-title":"Ethiopia. BMC Vet Res"},{"issue":"4","key":"pone.0287902.ref074","doi-asserted-by":"crossref","first-page":"164","DOI":"10.21825\/vdt.v83i4.16642","article-title":"Prevalence of Chlamydia abortus in Belgian ruminants.","volume":"83","author":"L Yin","year":"2014","journal-title":"Vlaams Diergeneeskundig Tijdschrift."},{"key":"pone.0287902.ref075","doi-asserted-by":"crossref","first-page":"101500","DOI":"10.1016\/j.cimid.2020.101500","article-title":"Seroprevalence and associated risk factors of Chlamydia abortus infection in ewes in Tunisia","volume":"71","author":"A Mamlouk","year":"2020","journal-title":"Comparative Immunology, Microbiology and Infectious Diseases"},{"issue":"1","key":"pone.0287902.ref076","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s40588-015-0014-2","article-title":"Chlamydia abortus: New Aspects of Infectious Abortion in Sheep and Potential Risk for Pregnant Women.","volume":"2","author":"A Essig","year":"2015","journal-title":"Curr Clin Micro Rpt."},{"issue":"2","key":"pone.0287902.ref077","first-page":"4","article-title":"Enzootic abortion of small ruminants","volume":"23","author":"L. Prozesky","year":"2019","journal-title":"The Review: The Livestock Health and Production Review, Vetlink Publications."},{"issue":"12","key":"pone.0287902.ref078","doi-asserted-by":"crossref","first-page":"460","DOI":"10.17221\/5740-VETMED","article-title":"Seroprevalence of antibodies to chlamydophila abortus shown in awassi sheep and local goats in jordan.","volume":"49","author":"KM Al-Qudah","year":"2004","journal-title":"Veterin\u00e1rn\u00ed Medic\u00edna."},{"key":"pone.0287902.ref079","volume-title":"Seroprevalence survey of Chlamydophila abortus infection in breeding goats on commercial farms in northern Namibia","author":"A. Samkange","year":"2008"},{"issue":"2","key":"pone.0287902.ref080","doi-asserted-by":"crossref","first-page":"63","DOI":"10.4102\/jsava.v72i2.617","article-title":"Chlamydial abortion in goats in the Umzimkulu District of Eastern Cape: to the editor","volume":"72","author":"CS Jeyakumar","year":"2001","journal-title":"Journal of the South African Veterinary Association"},{"key":"pone.0287902.ref081","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1002\/9780470753316.ch16","volume-title":"Chlamydial Abortion. In: Diseases of Sheep","author":"ID Aitken","year":"2007","edition":"4"},{"issue":"3","key":"pone.0287902.ref082","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1007\/s11250-010-9761-5","article-title":"Milk producers\u2019 awareness of milk-borne zoonoses in selected smallholder and commercial dairy farms of Zimbabwe.","volume":"43","author":"D Mosalagae","year":"2011","journal-title":"Trop Anim Health Prod."},{"issue":"2","key":"pone.0287902.ref083","first-page":"175","article-title":"DETERMINATION OF RISK FACTORS AND LEVEL OF AWARENESS OF CAPRINE BRUCELLOSIS AMONGST GOAT OWNERS IN OJU LGA, BENUE STATE, NIGERIA.","volume":"62","author":"GO Ijale","year":"2014","journal-title":"Bulletin of Animal Health and Production in Africa."},{"key":"pone.0287902.ref084","doi-asserted-by":"crossref","DOI":"10.1596\/33471","article-title":"Zimbabwe Smallholder Agricultural Productivity Survey 2017 Report","author":"World Bank ZimStat","year":"2019","journal-title":"Zimbabwe National Statistics Agency"},{"issue":"1","key":"pone.0287902.ref085","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.pt.2008.09.008","article-title":"Toxoplasma-safe meat: close to reality?","volume":"25","author":"A Kijlstra","year":"2009","journal-title":"Trends in Parasitology"},{"issue":"12","key":"pone.0287902.ref086","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1016\/j.ijpara.2008.06.002","article-title":"Control of the risk of human toxoplasmosis transmitted by meat","volume":"38","author":"A Kijlstra","year":"2008","journal-title":"International Journal for Parasitology"},{"key":"pone.0287902.ref087","first-page":"64","article-title":"Abortion in woman caused by caprine Chlamydophila abortus (Chlamydia psittaci serovar 1).","volume":"132","author":"A Pospischil","year":"2002","journal-title":"Swiss medical weekly."},{"issue":"4","key":"pone.0287902.ref088","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1002\/vms3.190","article-title":"Seroprevalence and characterization of Brucella species in cattle slaughtered at Gauteng abattoirs, South Africa.","volume":"5","author":"FB Kolo","year":"2019","journal-title":"Veterinary Medicine and Science"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0287902","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T18:18:29Z","timestamp":1688062709000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0287902"}},"subtitle":[],"editor":[{"given":"Sherin Reda","family":"Rouby","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,6,29]]},"references-count":88,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0287902","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,29]]}}}