{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:17:49Z","timestamp":1792801069220,"version":"4.5.0"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sichuan University West China Hospital","award":["setup funding"],"award-info":[{"award-number":["setup funding"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Testicular torsion is a urological emergency. However, surgical detorsion of the torsed spermatic cord can cause testicular reperfusion injury. Although remote ischemic preconditioning (RIPC) has been convincingly shown to protect organs against ischemia\/reperfusion (I\/R) injury, little is known regarding the effect of RIPC on testicular torsion\/detorsion-induced reperfusion injury. Therefore, we aimed to evaluate the effect of RIPC on testes after testicular I\/R injury in a rat model <jats:italic>in vivo<\/jats:italic>. Male Sprague\u2013Dawley rats were randomly classified into 4 groups: sham-operated (sham), testicular I\/R (TI\/R), or remote liver (RIPC liver) and limb (RIPC limb) ischemic preconditioning groups. Testis I\/R was induced by 3 h of right spermatic cord torsion (720\u00b0 clockwise), and reperfusion was allowed for 3 hours. In the RIPC group, four cycles of 5 min of ischemia and 5 min of reperfusion were completed 30 min prior to testicular torsion. The ERK1\/2 inhibitor U0126 was administered intravenously at the beginning of reperfusion (1 mg\/kg). The testes were taken for the oxidative stress evaluations, histology, apoptosis, immunohistochemical and western blotting analysis. Remote liver and limb ischemic preconditioning attenuated ipsilateral and contralateral testicular damage after testicular I\/R injury. For example. RIPC reduced testicular swelling and oxidative stress, lessened structural damage, and inhibited the testicular inflammatory response and apoptosis. Furthermore, RIPC treatment enhanced testicular ERK1\/2 phosphorylation postI\/R. Inhibition of ERK1\/2 activity using U0126 eliminated the protection offered by RIPC. Our data demonstrate for the first time that RIPC protects testes against testicular I\/R injury via activation of the ERK1\/2 signaling pathway.<\/jats:p>","DOI":"10.1371\/journal.pone.0287987","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T17:38:04Z","timestamp":1698341884000},"page":"e0287987","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Pretreatment with remote ischemic conditioning attenuates testicular damage after testicular ischemia and reperfusion injury in 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