{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T22:14:24Z","timestamp":1785190464347,"version":"3.55.0"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T00:00:00Z","timestamp":1690761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015747","name":"University Grants Commission of Bangladesh","doi-asserted-by":"publisher","award":["37-01-0000-073-06-014-19\/1537"],"award-info":[{"award-number":["37-01-0000-073-06-014-19\/1537"]}],"id":[{"id":"10.13039\/100015747","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Grant of Advanced Research in Education, Ministry of Education, Bangladesh","award":["37.20.0000.004.033.020.2016.1053"],"award-info":[{"award-number":["37.20.0000.004.033.020.2016.1053"]}]},{"name":"Special allocation, Ministry of Science and Technology, Bangladesh","award":["39.00.0000.009.99.024.22-901"],"award-info":[{"award-number":["39.00.0000.009.99.024.22-901"]}]},{"name":"Basic Research Grant of Bangladesh University of Engineering and Technology","award":["Est\/R-60\/Re-5336"],"award-info":[{"award-number":["Est\/R-60\/Re-5336"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The hydrophilic polymer polyethylene glycol-grafted phospholipid has been used extensively in the study of artificial vesicles, nanomedicine, and antimicrobial peptides\/proteins. In this research, the effects of 1,2-dioleoyl-<jats:italic>sn<\/jats:italic>-glycero-3-phosphoethanolamine-N- [methoxy (polyethylene glycol)-2000] (abbreviated PEG-DOPE) on the deformation and poration of giant unilamellar vesicles (GUVs)-induced by anionic magnetite nanoparticles (NPs) have been investigated. For this, the size of the NPs used was 18 nm, and their concentration in the physiological solution was 2.00 \u03bcg\/mL. GUVs were prepared using the natural swelling method comprising 1,2-dioleoyl-<jats:italic>sn<\/jats:italic>-glycero-3-phosphocholine (DOPC) and PEG-DOPE. The mole% of PEG-DOPE in the membranes were 0, 2, and 5%. The degree of deformation of the GUVs was quantified by the parameter compactness (<jats:italic>C<\/jats:italic><jats:sub>om<\/jats:sub>), which is 1.0 for the spherical-shaped GUVs. The value of<jats:italic>C<\/jats:italic><jats:sub>om<\/jats:sub>increases with time during the interactions of NPs with GUVs for any concentration of PEG-DOPE, but the rate of increase is significantly influenced by the PEG-DOPE concentration in the membranes. The average compactness increases with the increase of PEG-DOPE%, and after 60 min of NPs interaction, the values of average compactness for 0, 2, and 5% PEG-DOPE were 1.19 \u00b1 0.02, 1.26 \u00b1 0.03 and 1.35 \u00b1 0.05, respectively. The fraction of deformation (<jats:italic>Fr<\/jats:italic><jats:sub>d<\/jats:sub>) also increased with the increase of PEG-DOPE%, and at 60 min, the values of<jats:italic>Fr<\/jats:italic><jats:sub>d<\/jats:sub>for 0 and 5% PEG-DOPE were 0.47 \u00b1 0.02 and 0.63 \u00b1 0.02, respectively. The fraction of poration (<jats:italic>Fr<\/jats:italic><jats:sub>p<\/jats:sub>) increased with the increase of PEG-DOPE, and at 60 min, the values of<jats:italic>Fr<\/jats:italic><jats:sub>p<\/jats:sub>for 0 and 5% PEG-DOPE were 0.25 \u00b1 0.02 and 0.48 \u00b1 0.02, respectively. Hence, the presence of PEG-grafted phospholipid in the membranes greatly enhances the anionic magnetite NPs-induced deformation and poration of giant vesicles.<\/jats:p>","DOI":"10.1371\/journal.pone.0289087","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T17:22:13Z","timestamp":1690824133000},"page":"e0289087","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of polyethylene glycol-grafted phospholipid on the anionic magnetite nanoparticles-induced deformation and poration in giant lipid vesicles"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5288-2357","authenticated-orcid":true,"given":"Mohammad Abu Sayem","family":"Karal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharmin","family":"Sultana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3950-5597","authenticated-orcid":true,"given":"Md. Masum","family":"Billah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9469-4635","authenticated-orcid":true,"given":"Md.","family":"Moniruzzaman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Abdul","family":"Wadud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. C.","family":"Gosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,7,31]]},"reference":[{"key":"pone.0289087.ref001","doi-asserted-by":"crossref","first-page":"7569","DOI":"10.1039\/D2SM00806H","article-title":"Impact of poly(ethylene glycol) functionalized lipids on ordering and fluidity of colloid supported lipid bilayers","volume":"18","author":"EC Giakoumatos","year":"2022","journal-title":"Soft Matter"},{"key":"pone.0289087.ref002","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.chemphyslip.2018.12.001","article-title":"PEG-grafted phospholipids in vesicles: Effect of PEG chain length and concentration on mechanical properties","volume":"218","author":"A Mahendra","year":"2019","journal-title":"Chem Phys Lipids"},{"key":"pone.0289087.ref003","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0005-2736(03)00197-4","article-title":"Lipid membranes with grafted polymers: physicochemical aspects","volume":"1615","author":"D Marsh","year":"2003","journal-title":"Biochim Biophys Acta (BBA)\u2014Biomembr"},{"key":"pone.0289087.ref004","doi-asserted-by":"crossref","first-page":"38","DOI":"10.3390\/membranes7030038","article-title":"Artificial lipid membranes: past, present, and future","volume":"7","author":"CG Siontorou","year":"2017","journal-title":"Membr"},{"key":"pone.0289087.ref005","article-title":"Artificial and Natural Membranes. Atomic Force Microscopy Investigations into Biology\u2014From Cell to Protein","author":"G V\u00e1r\u00f3","year":"2012","journal-title":"IntechOpen"},{"key":"pone.0289087.ref006","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3390\/scipharm89020015","article-title":"Simple equations pertaining to the particle number and surface area of metallic, polymeric, lipidic and vesicular nanocarriers","volume":"89","author":"MR Mozafari","year":"2021","journal-title":"Sci Pharm"},{"key":"pone.0289087.ref007","doi-asserted-by":"crossref","first-page":"8572","DOI":"10.1039\/C8CS00162F","article-title":"Liposomes and polymersomes: a comparative review towards cell mimicking","volume":"47","author":"E Rideau","year":"2018","journal-title":"Chem Soc Rev"},{"key":"pone.0289087.ref008","doi-asserted-by":"crossref","first-page":"2380","DOI":"10.1073\/pnas.0712125105","article-title":"SNAREpin\/Munc18 promotes adhesion and fusion of large vesicles to giant membranes","volume":"105","author":"D Tareste","year":"2008","journal-title":"Proc Natl Acad Sci"},{"key":"pone.0289087.ref009","doi-asserted-by":"crossref","first-page":"13992","DOI":"10.1039\/C9RA02462J","article-title":"Anionic nanoparticle-lipid membrane interactions: the protonation of anionic ligands at the membrane surface reduces membrane disruption","volume":"9","author":"S Salassi","year":"2019","journal-title":"RSC Adv"},{"key":"pone.0289087.ref010","doi-asserted-by":"crossref","first-page":"133","DOI":"10.2147\/IJN.S596","article-title":"Drug delivery and nanoparticles: Applications and hazards","volume":"3","author":"WHD Jong","year":"2008","journal-title":"IJN"},{"key":"pone.0289087.ref011","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s12951-018-0392-8","article-title":"Nano based drug delivery systems: recent developments and future prospects","volume":"16","author":"JK Patra","year":"2018","journal-title":"J Nanobiotechnol"},{"key":"pone.0289087.ref012","doi-asserted-by":"crossref","first-page":"5421","DOI":"10.1021\/nn1010792","article-title":"Penetration of lipid membranes by gold nanoparticles: insights into cellular uptake, cytotoxicity, and their relationship","volume":"4","author":"J Lin","year":"2010","journal-title":"ACS Nano"},{"key":"pone.0289087.ref013","doi-asserted-by":"crossref","first-page":"5577","DOI":"10.2147\/IJN.S36111","article-title":"The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles","volume":"7","author":"E. Fr\u00f6hlich","year":"2012","journal-title":"Int J Nanomed"},{"key":"pone.0289087.ref014","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1038\/s41598-020-80953-3","article-title":"Non-disruptive uptake of anionic and cationic gold nanoparticles in neutral zwitterionic membranes","volume":"11","author":"E Canepa","year":"2021","journal-title":"Sci Rep"},{"key":"pone.0289087.ref015","doi-asserted-by":"crossref","first-page":"1696","DOI":"10.2174\/1389450119666180326122831","article-title":"Nanoparticles in cancer treatment: opportunities and obstacles","volume":"19","author":"R Awasthi","year":"2018","journal-title":"Curr Drug Targets"},{"key":"pone.0289087.ref016","doi-asserted-by":"crossref","first-page":"3280","DOI":"10.1002\/anie.200904359","article-title":"Gold nanoparticles for biology and medicine","volume":"49","author":"DA Giljohann","year":"2010","journal-title":"Angew Chem Int Ed Engl"},{"key":"pone.0289087.ref017","doi-asserted-by":"crossref","first-page":"113","DOI":"10.2217\/17435889.2.1.113","article-title":"Functionalized gold nanoparticles for drug delivery","volume":"2","author":"G Han","year":"2007","journal-title":"Nanomedicine (Lond)"},{"key":"pone.0289087.ref018","article-title":"Nanoparticles in the treatment of infections caused by multidrug-resistant organisms","volume":"10","author":"Y Lee N-","year":"2019","journal-title":"Front Pharmacol"},{"key":"pone.0289087.ref019","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s11051-023-05690-w","article-title":"Structural parameters of nanoparticles affecting their toxicity for biomedical applications: a review","volume":"25","author":"R Abbasi","year":"2023","journal-title":"J Nanopart Res"},{"key":"pone.0289087.ref020","doi-asserted-by":"crossref","first-page":"e9954443","DOI":"10.1155\/2021\/9954443","article-title":"Toxicity of nanoparticles in biomedical application: nanotoxicology","volume":"2021","author":"C Egbuna","year":"2021","journal-title":"J Toxicol"},{"key":"pone.0289087.ref021","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.addr.2009.11.008","article-title":"Better safe than sorry: Understanding the toxicological properties of inorganic nanoparticles manufactured for biomedical applications","volume":"62","author":"B Fadeel","year":"2010","journal-title":"Adv Drug Deliv Rev"},{"key":"pone.0289087.ref022","doi-asserted-by":"crossref","first-page":"100119","DOI":"10.1016\/j.medidd.2021.100119","article-title":"Blood cells as supercarrier systems for advanced drug delivery","volume":"13","author":"S Wang","year":"2022","journal-title":"Med Drug Discov"},{"key":"pone.0289087.ref023","doi-asserted-by":"crossref","unstructured":"Cruz GGDL, Rodr\u00edguez-Fragoso P, Reyes-Esparza J, Rodr\u00edguez-L\u00f3pez A, Rodriguez-Fragoso RG-C and L, Cruz GGDL, et al. Interaction of nanoparticles with blood components and associated pathophysiological effects. Unraveling the safety profile of nanoscale particles and materials\u2014from biomedical to environmental applications. IntechOpen; 2017.","DOI":"10.5772\/intechopen.69386"},{"key":"pone.0289087.ref024","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1093\/toxsci\/kfr233","article-title":"Susceptibility to inhaled flame-generated ultrafine soot in neonatal and adult rat lungs","volume":"124","author":"JKW Chan","year":"2011","journal-title":"Toxicol Sci"},{"key":"pone.0289087.ref025","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1477-3155-2-12","article-title":"Nanoparticles\u2013known and unknown health risks","volume":"2","author":"PH Hoet","year":"2004","journal-title":"J Nanobiotechnol"},{"key":"pone.0289087.ref026","doi-asserted-by":"crossref","first-page":"MR17","DOI":"10.1116\/1.2815690","article-title":"Nanomaterials and nanoparticles: Sources and toxicity","volume":"2","author":"C Buzea","year":"2007","journal-title":"Biointerphases"},{"key":"pone.0289087.ref027","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.7150\/thno.23856","article-title":"Applications of gold nanoparticles in virus detection","volume":"8","author":"MS Draz","year":"2018","journal-title":"Theranostics"},{"key":"pone.0289087.ref028","doi-asserted-by":"crossref","first-page":"e00114","DOI":"10.1128\/spectrum.00114-22","article-title":"Single-Cell analysis of the antimicrobial and bactericidal activities of the antimicrobial peptide magainin 2","volume":"10","author":"F Hossain","year":"2022","journal-title":"Microbiol Spect"},{"key":"pone.0289087.ref029","doi-asserted-by":"crossref","first-page":"6716","DOI":"10.1039\/D1CP05764B","article-title":"Effect of osmotic pressure on pore formation in lipid bilayers by the antimicrobial peptide magainin 2","volume":"24","author":"MM Billah","year":"2022","journal-title":"Phys Chem Chem Phys"},{"key":"pone.0289087.ref030","doi-asserted-by":"crossref","first-page":"3349","DOI":"10.1021\/acs.langmuir.7b04219","article-title":"Mechanism of initial stage of pore formation induced by antimicrobial peptide magainin 2","volume":"34","author":"M Hasan","year":"2018","journal-title":"Langmuir"},{"key":"pone.0289087.ref031","doi-asserted-by":"crossref","first-page":"13487","DOI":"10.1039\/C6CP01184E","article-title":"Analysis of constant tension-induced rupture of lipid membranes using activation energy","volume":"18","author":"MAS Karal","year":"2016","journal-title":"Phys Chem Chem Phys"},{"key":"pone.0289087.ref032","doi-asserted-by":"crossref","first-page":"081103","DOI":"10.1063\/1.4930108","article-title":"Communication: Activation energy of tension-induced pore formation in lipid membranes","volume":"143","author":"MAS Karal","year":"2015","journal-title":"J Chem Phys"},{"key":"pone.0289087.ref033","doi-asserted-by":"crossref","first-page":"2190","DOI":"10.1016\/j.bpj.2016.09.043","article-title":"Experimental estimation of membrane tension induced by osmotic pressure","volume":"111","author":"SUA Shibly","year":"2016","journal-title":"Biophys J"},{"key":"pone.0289087.ref034","doi-asserted-by":"crossref","first-page":"e0251690","DOI":"10.1371\/journal.pone.0251690","article-title":"Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles","volume":"16","author":"MK Sarkar","year":"2021","journal-title":"PLOS ONE"},{"key":"pone.0289087.ref035","doi-asserted-by":"crossref","first-page":"184112","DOI":"10.1016\/j.bbamem.2022.184112","article-title":"Antimicrobial peptide magainin 2-induced rupture of single giant unilamellar vesicles comprising E. coli polar lipids","volume":"1865","author":"MdM Billah","year":"2023","journal-title":"Biochim Biophys Acta (BBA)\u2014Biomembr"},{"key":"pone.0289087.ref036","doi-asserted-by":"crossref","first-page":"e0262555","DOI":"10.1371\/journal.pone.0262555","article-title":"Quantification of pulsed electric field for the rupture of giant vesicles with various surface charges, cholesterols and osmotic pressures","volume":"17","author":"MK Ahamed","year":"2022","journal-title":"PLOS ONE"},{"key":"pone.0289087.ref037","doi-asserted-by":"crossref","first-page":"012404","DOI":"10.1103\/PhysRevE.101.012404","article-title":"Electrostatic interaction effects on the size distribution of self-assembled giant unilamellar vesicles","volume":"101","author":"MAS Karal","year":"2020","journal-title":"Phys Rev E"},{"key":"pone.0289087.ref038","doi-asserted-by":"crossref","unstructured":"Yamazaki, M. Advances in planar lipid bilayers and liposomes; Leitmannova, A. L. Ed.; Elsevier\/Academic Press: London, 2008, Pages 121\u2013142 (Chapter 5).","DOI":"10.1016\/S1554-4516(08)00005-7"},{"key":"pone.0289087.ref039","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1080\/17458080.2017.1413253","article-title":"Nanoparticle\u2013membrane interactions","volume":"13","author":"C Contini","year":"2018","journal-title":"J Exp Nanosci"},{"key":"pone.0289087.ref040","doi-asserted-by":"crossref","first-page":"7692","DOI":"10.1021\/nn3012042","article-title":"Facile Preparation of cationic gold nanoparticle-bioconjugates for cell penetration and nuclear targeting","volume":"6","author":"I Ojea-Jim\u00e9nez","year":"2012","journal-title":"ACS Nano"},{"key":"pone.0289087.ref041","first-page":"012034","article-title":"A study on the interaction of nanoparticles with lipid membranes and their influence on membrane fluidity","volume":"398","author":"PB Santhosh","year":"2012","journal-title":"J Phys: Conf Ser"},{"key":"pone.0289087.ref042","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1021\/bm700535p","article-title":"Surface charge of nanoparticles determines their endocytic and transcytotic pathway in polarized MDCK cells","volume":"9","author":"O Harush-Frenkel","year":"2008","journal-title":"Biomacromolecules"},{"key":"pone.0289087.ref043","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/B978-0-08-055294-1.00150-1","volume-title":"Comprehensive Biomaterials","author":"H Akita","year":"2011"},{"key":"pone.0289087.ref044","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1248\/cpb.51.815","article-title":"Effects of poly(ethylene glycol) (PEG) chain length of PEG-lipid on the permeability of liposomal bilayer membranes","volume":"51","author":"K Hashizaki","year":"2003","journal-title":"Chem Pharm Bull"},{"key":"pone.0289087.ref045","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/jcp.1040730108","article-title":"Formation and properties of thin-walled phospholipid vesicles","volume":"73","author":"JP Reeves","year":"1969","journal-title":"J Cell Physiol"},{"key":"pone.0289087.ref046","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.chemphyslip.2011.04.003","article-title":"A membrane filtering method for the purification of giant unilamellar vesicles","volume":"164","author":"Y Tamba","year":"2011","journal-title":"Chem Phys Lipids"},{"key":"pone.0289087.ref047","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s00249-019-01363-6","article-title":"Low cost non-electromechanical technique for the purification of giant unilamellar vesicles","volume":"48","author":"MAS Karal","year":"2019","journal-title":"Eur Biophys J"},{"key":"pone.0289087.ref048","doi-asserted-by":"crossref","first-page":"7824","DOI":"10.1002\/slct.201901594","article-title":"Eco-friendly synthesis of Fe3O4 nanoparticles based on natural stabilizers and their antibacterial applications","volume":"4","author":"MM Zaman","year":"2019","journal-title":"ChemistrySelect"},{"key":"pone.0289087.ref049","doi-asserted-by":"crossref","first-page":"104916","DOI":"10.1016\/j.chemphyslip.2020.104916","article-title":"Deformation and poration of giant unilamellar vesicles induced by anionic nanoparticles","volume":"230","author":"MAS Karal","year":"2020","journal-title":"Chem Phys Lipids"},{"key":"pone.0289087.ref050","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/S0301-5629(01)00360-X","article-title":"Handbook of Medical Imaging, Processing and Analysis: Edited by Isaac N. Bankman, PhD, Academic Press, 2000, ISBN 0-12-077790-8, 901 pp","volume":"27","author":"A. Fenster","year":"2001","journal-title":"Ultrasound in Medicine and Biology"},{"key":"pone.0289087.ref051","doi-asserted-by":"crossref","first-page":"3637","DOI":"10.3390\/s21113637","article-title":"Discrimination and quantitation of biologically relevant carboxylate anions using A [Dye\u2022PAMAM] Complex","volume":"21","author":"Y Xu","year":"2021","journal-title":"Sensors"},{"key":"pone.0289087.ref052","doi-asserted-by":"crossref","first-page":"15752","DOI":"10.1039\/C4CP00717D","article-title":"The single GUV method for revealing the functions of antimicrobial, pore-forming toxin, and cell-penetrating peptides or proteins","volume":"16","author":"MZ Islam","year":"2014","journal-title":"Phys Chem Chem Phys"},{"key":"pone.0289087.ref053","doi-asserted-by":"crossref","first-page":"28283","DOI":"10.1039\/D2RA03199J","article-title":"Effects of cholesterol on the anionic magnetite nanoparticle-induced deformation and poration of giant lipid vesicles","volume":"12","author":"S Akter","year":"2022","journal-title":"RSC Adv"},{"key":"pone.0289087.ref054","doi-asserted-by":"crossref","first-page":"3391","DOI":"10.1021\/la503318z","article-title":"Stretch-activated pore of the antimicrobial peptide, magainin 2","volume":"31","author":"MAS Karal","year":"2015","journal-title":"Langmuir"},{"key":"pone.0289087.ref055","doi-asserted-by":"crossref","first-page":"e0275478","DOI":"10.1371\/journal.pone.0275478","article-title":"Influence of sugar concentration on the vesicle compactness, deformation and membrane poration induced by anionic nanoparticles","volume":"17","author":"S Hasan","year":"2022","journal-title":"PLOS ONE"},{"key":"pone.0289087.ref056","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s12551-020-00662-z","article-title":"Action of antimicrobial peptides and cell-penetrating peptides on membrane potential revealed by the single GUV method","volume":"12","author":"MMR Moghal","year":"2020","journal-title":"Biophys Rev"},{"key":"pone.0289087.ref057","doi-asserted-by":"crossref","first-page":"4846","DOI":"10.1021\/jp8109622","article-title":"Magainin 2-induced pore formation in the lipid membranes depends on its concentration in the membrane interface","volume":"113","author":"Y Tamba","year":"2009","journal-title":"J Phys Chem B"},{"key":"pone.0289087.ref058","doi-asserted-by":"crossref","first-page":"18171","DOI":"10.1073\/pnas.0807296105","article-title":"Nanoparticle-induced surface reconstruction of phospholipid membranes","volume":"105","author":"B Wang","year":"2008","journal-title":"P Proc Natl Acad Sci"},{"key":"pone.0289087.ref059","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1103\/PhysRevA.44.1182","article-title":"Shape transformations of vesicles: Phase diagram for spontaneous- curvature and bilayer-coupling models","volume":"44","author":"U Seifert","year":"1991","journal-title":"Phys Rev A"},{"key":"pone.0289087.ref060","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1016\/S0006-3495(91)82117-8","article-title":"Shape transitions and shape stability of giant phospholipid vesicles in pure water induced by area-to-volume changes","volume":"60","author":"J K\u00e4s","year":"1991","journal-title":"Biophys J"},{"key":"pone.0289087.ref061","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1016\/S0021-9290(99)00136-0","article-title":"Cylindrical shapes of closed lipid bilayer structures correspond to an extreme area difference between the two monolayers of the bilayer","volume":"32","author":"A Iglic","year":"1999","journal-title":"J Biomech"},{"key":"pone.0289087.ref062","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/BF00257107","article-title":"Membrane bending energy and shape determination of phospholipid vesicles and red blood cells","volume":"17","author":"S Svetina","year":"1989","journal-title":"Eur Biophys J"},{"key":"pone.0289087.ref063","doi-asserted-by":"crossref","first-page":"3112","DOI":"10.1103\/PhysRevE.48.3112","article-title":"Nonaxisymmetric vesicle shapes in a generalized bilayer-couple model and the transition between oblate and prolate axisymmetric shapes","volume":"48","author":"V Heinrich","year":"1993","journal-title":"Phys Rev E"},{"key":"pone.0289087.ref064","doi-asserted-by":"crossref","first-page":"5389","DOI":"10.1103\/PhysRevE.49.5389","article-title":"Budding transitions of fluid-bilayer vesicles: The effect of area-difference elasticity","volume":"49","author":"L Miao","year":"1994","journal-title":"Phys Rev E"},{"key":"pone.0289087.ref065","doi-asserted-by":"crossref","first-page":"112758","DOI":"10.1016\/j.cma.2019.112758","article-title":"Bending models of lipid bilayer membranes: Spontaneous curvature and area-difference elasticity","volume":"359","author":"X Bian","year":"2020","journal-title":"Comput Methods Appl Mech Eng"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0289087","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T05:31:22Z","timestamp":1702877482000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0289087"}},"subtitle":[],"editor":[{"given":"Massimiliano","family":"Papi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,7,31]]},"references-count":65,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,7,31]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0289087","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,31]]}}}