{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T22:43:27Z","timestamp":1768776207742,"version":"3.49.0"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006360","name":"Federal Ministry for Economic Affairs and Energy","doi-asserted-by":"crossref","award":["ZIM 16KN051655"],"award-info":[{"award-number":["ZIM 16KN051655"]}],"id":[{"id":"10.13039\/501100006360","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006360","name":"Federal Ministry for Economic Affairs and Energy","doi-asserted-by":"crossref","award":["ZIM 16KN051656"],"award-info":[{"award-number":["ZIM 16KN051656"]}],"id":[{"id":"10.13039\/501100006360","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Saxon State Government out of the state budget approved by the Saxon State Parliament","award":["31004 70 809"],"award-info":[{"award-number":["31004 70 809"]}]},{"name":"Open Access Publishing Fund of Leipzig University"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The complexity of the osseo-ligamentous lumbopelvic system has made it difficult to perform both, the overall preparation as well as specimen harvesting and material testing with a reasonable amount of time and personnel. The logistics of such studies present a hurdle for reproducibility. A structured procedure was developed and proved, which allows all necessary steps to be carried out reproducibly and in a reasonable time. This enables the extraction of 26 soft tissue, 33 trabecular and 32 cortical bone specimens from this anatomical region per cadaver. The integrity of the specimens remains maintained while keeping requirements within manageable limits. The practicability of the intended five-day specimen harvesting and testing procedure could be demonstrated on five test and two pre-test sequences. The intended minimization of physical, biological, and chemical external influences on specimens could be achieved. All protocols, instructions and models of preparation and storage devices are included in the supporting information. The high grade of applicability and reproducibility will lead to better comparability between different biomechanical investigations. This procedure proven on the human pelvis is transferable to other anatomical regions.<\/jats:p>","DOI":"10.1371\/journal.pone.0289482","type":"journal-article","created":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T17:32:19Z","timestamp":1691083939000},"page":"e0289482","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Approach to standardized material characterization of the human lumbopelvic system\u2014Specification, preparation and storage"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0797-2246","authenticated-orcid":true,"given":"Sascha","family":"Kurz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8378-3108","authenticated-orcid":true,"given":"Marc","family":"Gebhardt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanny","family":"Grundmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph-Eckhard","family":"Heyde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanno","family":"Steinke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,8,3]]},"reference":[{"key":"pone.0289482.ref001","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1111\/j.1469-7580.2012.01564.x","article-title":"The sacroiliac joint: an overview of its anatomy, function and potential clinical implications","volume":"221","author":"A Vleeming","year":"2012","journal-title":"J Anat"},{"key":"pone.0289482.ref002","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1097\/00007632-199002000-00017","article-title":"Relation between form and function in the sacroiliac joint, part II: Biomechanical aspects","author":"A Vleeming","year":"1990","journal-title":"Spine"},{"key":"pone.0289482.ref003","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0268-0033(93)90002-Y","article-title":"Transfer of lumbosacral load to iliac bones and legs","volume":"8","author":"CJ Snijders","year":"1993","journal-title":"Clin Biomech (Bristol, Avon)"},{"key":"pone.0289482.ref004","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1016\/j.spinee.2013.03.050","article-title":"Ligamentous influence in pelvic load distribution","volume":"13","author":"N Hammer","year":"2013","journal-title":"Spine J"},{"key":"pone.0289482.ref005","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1055\/s-0042-109409","article-title":"Biomechanik des Beckens","volume":"12","author":"S Kurz","year":"2017","journal-title":"Orthop\u00e4die und Unfallchirurgie up2date"},{"key":"pone.0289482.ref006","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jbiomech.2008.10.005","article-title":"Which axial and bending stiffnesses of posterior implants are required to design a flexible lumbar stabilization system","volume":"42","author":"H Schmidt","year":"2009","journal-title":"Journal of Biomechanics"},{"key":"pone.0289482.ref007","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.prosdent.2004.10.001","article-title":"Three-dimensional finite-element analysis of functional stresses in different bone locations produced by implants placed in the maxillary posterior region of the sinus floor","volume":"93","author":"OL Koca","year":"2005","journal-title":"J Prosthet Dent"},{"key":"pone.0289482.ref008","doi-asserted-by":"crossref","first-page":"e8136","DOI":"10.1097\/MD.0000000000008136","article-title":"Three-dimensional reduction and finite element analysis improves the treatment of pelvic malunion reconstructive surgery: A case report. A case report","volume":"96","author":"S Kurz","year":"2017","journal-title":"Medicine (Baltimore)"},{"key":"pone.0289482.ref009","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/0021-9290(94)00125-N","article-title":"Load transfer across the pelvic bone","volume":"28","author":"M Dalstra","year":"1995","journal-title":"Journal of Biomechanics"},{"key":"pone.0289482.ref010","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1097\/BRS.0b013e318191ea01","article-title":"Anatomical and biomechanical analyses of the unique and consistent locations of sacral insufficiency fractures","volume":"34","author":"NJ Linstrom","year":"2009","journal-title":"Spine"},{"key":"pone.0289482.ref011","unstructured":"Wolff J. Das Gesetz der Transformation der Knochen. 1st ed. Berlin: Pro Business; 2010."},{"key":"pone.0289482.ref012","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1115\/1.1894148","article-title":"Subject-specific finite element model of the pelvis: development, validation and sensitivity studies","volume":"127","author":"AE Anderson","year":"2005","journal-title":"Journal of Biomechanical Engineering"},{"key":"pone.0289482.ref013","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/0021-9290(93)90014-6","article-title":"Mechanical and textural properties of pelvic trabecular bone","volume":"26","author":"M Dalstra","year":"1993","journal-title":"Journal of Biomechanics"},{"key":"pone.0289482.ref014","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1515\/cdbme-2019-0087","article-title":"Kinematics of the Lumbo\u2013Pelvic Complex under Different Loading Conditions","volume":"5","author":"M Weidling","year":"2019","journal-title":"Current Directions in Biomedical Engineering"},{"key":"pone.0289482.ref015","first-page":"20.1","volume-title":"Bone mechanics handbook","author":"RB Martin","year":"2001","edition":"2"},{"key":"pone.0289482.ref016","doi-asserted-by":"crossref","first-page":"14836","DOI":"10.1038\/s41598-020-71790-5","article-title":"The influence of different sample preparation on mechanical properties of human iliotibial tract","volume":"10","author":"B Fischer","year":"2020","journal-title":"Sci Rep"},{"key":"pone.0289482.ref017","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1007\/s00167-010-1278-y","article-title":"Effect of repeated freezing-thawing on the Achilles tendon of rabbits","volume":"19","author":"L Chen","year":"2011","journal-title":"Knee Surg Sports Traumatol Arthrosc"},{"key":"pone.0289482.ref018","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/nyas.13460","article-title":"Freezing does not alter multiscale tendon mechanics and damage mechanisms in tension","volume":"1409","author":"AH Lee","year":"2017","journal-title":"Ann N Y Acad Sci"},{"key":"pone.0289482.ref019","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.jbiomech.2017.08.018","article-title":"The influence of cryopreservation and quick-freezing on the mechanical properties of tendons","volume":"64","author":"I Oswald","year":"2017","journal-title":"Journal of Biomechanics"},{"key":"pone.0289482.ref020","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1002\/jor.21373","article-title":"The effects of multiple freeze-thaw cycles on the biomechanical properties of the human bone-patellar tendon-bone allograft","volume":"29","author":"H-J Jung","year":"2011","journal-title":"J Orthop Res"},{"key":"pone.0289482.ref021","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1002\/jor.1100130114","article-title":"Effects of freeze\/thaw conditioning on the tensile properties and failure mode of bone-muscle-bone units: a biomechanical and histological study in dogs","volume":"13","author":"F Gottsauner-Wolf","year":"1995","journal-title":"J Orthop Res"},{"key":"pone.0289482.ref022","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/j.bone.2010.08.012","article-title":"Effects of three different preservation methods on the mechanical properties of human and bovine cortical bone","volume":"47","author":"S Unger","year":"2010","journal-title":"Bone"},{"key":"pone.0289482.ref023","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.jmbbm.2013.09.008","article-title":"Ethanol and formaldehyde fixation irreversibly alter bones\u2019 organic matrix","volume":"29","author":"N Hammer","year":"2014","journal-title":"J Mech Behav Biomed Mater"},{"key":"pone.0289482.ref024","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0940-9602(01)80234-X","article-title":"Plastinated body slices for verification of magnetic resonance tomography images","volume":"183","author":"H. Steinke","year":"2001","journal-title":"Annals of Anatomy\u2014Anatomischer Anzeiger"},{"key":"pone.0289482.ref025","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0021-9290(88)90257-6","article-title":"Limitations of the continuum assumption in cancellous bone","volume":"21","author":"TP Harrigan","year":"1988","journal-title":"Journal of Biomechanics"},{"key":"pone.0289482.ref026","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1016\/0021-9290(87)90023-6","article-title":"The mechanical properties of trabecular bone. Dependence on anatomic location and function","volume":"20","author":"SA Goldstein","year":"1987","journal-title":"Journal of Biomechanics"},{"key":"pone.0289482.ref027","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/S0021-9290(98)00057-8","article-title":"Yield strain behavior of trabecular bone","volume":"31","author":"DL Kopperdahl","year":"1998","journal-title":"Journal of Biomechanics"},{"key":"pone.0289482.ref028","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jmbbm.2013.02.021","article-title":"Variability and anisotropy of mechanical behavior of cortical bone in tension and compression","volume":"21","author":"S Li","year":"2013","journal-title":"J Mech Behav Biomed Mater"},{"key":"pone.0289482.ref029","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.bone.2015.04.024","article-title":"Regional variation of bone tissue properties at the human mandibular condyle","volume":"77","author":"D-G Kim","year":"2015","journal-title":"Bone"},{"key":"pone.0289482.ref030","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.medengphy.2018.01.005","article-title":"Mechanical properties of cancellous bone from the acetabulum in relation to acetabular shell fixation and compared with the corresponding femoral head","volume":"53","author":"R van Ladesteijn","year":"2018","journal-title":"Med Eng Phys"},{"key":"pone.0289482.ref031","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s10237-008-0128-z","article-title":"Multi-axial mechanical properties of human trabecular bone","volume":"8","author":"L Rinc\u00f3n-Kohli","year":"2009","journal-title":"Biomech Model Mechanobiol"},{"key":"pone.0289482.ref032","doi-asserted-by":"crossref","first-page":"4599","DOI":"10.1118\/1.4730502","article-title":"Generalizing the mean intercept length tensor for gray-level images","volume":"39","author":"R Moreno","year":"2012","journal-title":"Med Phys"},{"key":"pone.0289482.ref033","first-page":"239","article-title":"Mechanical implications of collagen fibre orientation in cortical bone of the equine radius","volume":"187","author":"CM Riggs","year":"1993","journal-title":"Anat Embryol (Berl)"},{"key":"pone.0289482.ref034","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/BF02347055","article-title":"Anisotropy of Young\u2019s modulus of human tibial cortical bone","volume":"38","author":"BK Hoffmeister","year":"2000","journal-title":"Medical & Biological Engineering &Computing"},{"key":"pone.0289482.ref035","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s10237-010-0218-6","article-title":"Elastic anisotropy of bone lamellae as a function of fibril orientation pattern","volume":"10","author":"AG Reisinger","year":"2011","journal-title":"Biomech Model Mechanobiol"},{"key":"pone.0289482.ref036","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/joa.12227","article-title":"The functional coupling of the deep abdominal and paraspinal muscles: the effects of simulated paraspinal muscle contraction on force transfer to the middle and posterior layer of the thoracolumbar fascia","volume":"225","author":"A Vleeming","year":"2014","journal-title":"J Anat"},{"key":"pone.0289482.ref037","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1111\/j.1532-950X.2014.12247.x","article-title":"Biomechanical comparison of canine fascia lata and thoracolumbar fascia: an in vitro evaluation of replacement tissues for body wall reconstruction","volume":"44","author":"ER Henderson","year":"2015","journal-title":"Vet Surg"},{"key":"pone.0289482.ref038","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actbio.2018.08.001","article-title":"Young\u2019s modulus of trabecular bone at the tissue level. A review","volume":"78","author":"D Wu","year":"2018","journal-title":"Acta Biomater"},{"key":"pone.0289482.ref039","doi-asserted-by":"crossref","first-page":"e1176","DOI":"10.1002\/jsp2.1176","article-title":"Density and mechanical properties of vertebral trabecular bone-A review","volume":"4","author":"C \u00d6hman-M\u00e4gi","year":"2021","journal-title":"JOR Spine"},{"key":"pone.0289482.ref040","doi-asserted-by":"crossref","first-page":"104889","DOI":"10.1016\/j.jmbbm.2021.104889","article-title":"Biomechanics of immature human cortical bone: A systematic review","volume":"125","author":"E Szabo","year":"2022","journal-title":"J Mech Behav Biomed Mater"},{"key":"pone.0289482.ref041","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.clinbiomech.2007.08.024","article-title":"Mathematical relationships between bone density and mechanical properties. A literature review","volume":"23","author":"B Helgason","year":"2008","journal-title":"Clin Biomech (Bristol, Avon)"},{"key":"pone.0289482.ref042","article-title":"Atraumatic femoral head necrosis: a biomechanical, histological and radiological examination compared to primary hip osteoarthritis","author":"A Hofmann","year":"2021","journal-title":"Arch Orthop Trauma Surg"},{"key":"pone.0289482.ref043","doi-asserted-by":"crossref","first-page":"963689721999617","DOI":"10.1177\/0963689721999617","article-title":"Cryopreservation: An Overview of Principles and Cell-Specific Considerations","volume":"30","author":"D Whaley","year":"2021","journal-title":"Cell Transplant"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0289482","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T11:51:16Z","timestamp":1702900276000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0289482"}},"subtitle":[],"editor":[{"given":"JJ","family":"Cray","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,8,3]]},"references-count":43,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0289482","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,3]]}}}