{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:02:11Z","timestamp":1787022131481,"version":"build-2736575974"},"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:00Z","timestamp":1695772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Xichang University Doctoral Start-up Funding","award":["117281746"],"award-info":[{"award-number":["117281746"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Feed efficiency is a major constraint in the beef industry and has a significant negative correlation with residual feed intake (RFI). RFI is widely used as a measure of feed efficiency in beef cattle and is independent of economic traits such as body weight and average daily gain. However, key traits with commonality or specificity among beef cattle breeds at the same level of RFI have not been reported. Accordingly, the present study hypothesized that signatures associated with feed efficiency would have commonality or specificity in the liver of cattle breeds at the same RFI level. By comparing and integrating liver transcriptome data, we investigated the critical signatures closely associated with RFI in beef cattle using weighted co-expression network analysis, consensus module analysis, functional enrichment analysis and protein network interaction analysis. The results showed that the consensus modules in Angus and Charolais cattle were negatively correlated, and four (turquoise, red, tan, yellow) were significantly positively correlated in Angus liver, while (turquoise, red) were significantly negatively correlated in Charolais liver. These consensus modules were found to be primarily involved in biological processes such as substance metabolism, energy metabolism and gene transcription, which may be one of the possible explanations for the difference in feed efficiency between the two beef breeds. This research also identified five key candidate genes,\n                    <jats:italic>PLA2G12B<\/jats:italic>\n                    ,\n                    <jats:italic>LCAT<\/jats:italic>\n                    ,\n                    <jats:italic>MTTP<\/jats:italic>\n                    ,\n                    <jats:italic>LCAT<\/jats:italic>\n                    ,\n                    <jats:italic>ABCA1<\/jats:italic>\n                    and\n                    <jats:italic>FADS1<\/jats:italic>\n                    , which are closely associated with hepatic lipid metabolism. The present study has identified some modules, genes and pathways that may be the major contributors to the variation in feed efficiency among different cattle breeds, providing a new perspective on the molecular mechanisms of feed efficiency in beef cattle and a research basis for investigating molecular markers associated with feed efficiency in beef cattle.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0289939","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T13:34:10Z","timestamp":1695821650000},"page":"e0289939","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Characterization of feed efficiency-related key signatures molecular in different cattle breeds"],"prefix":"10.1371","volume":"18","author":[{"given":"Chaoyun","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengwen","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuili","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2644-4317","authenticated-orcid":true,"given":"Shuzhe","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,9,27]]},"reference":[{"issue":"7","key":"pone.0289939.ref001","doi-asserted-by":"crossref","first-page":"6131","DOI":"10.3168\/jds.2018-15407","article-title":"Defining the optimal period length and stage of growth or lactation to estimate residual feed intake in dairy cows","volume":"102","author":"EE Connor","year":"2019","journal-title":"J Dairy Sci"},{"key":"pone.0289939.ref002","doi-asserted-by":"crossref","first-page":"741878","DOI":"10.3389\/fgene.2021.741878","article-title":"Characterization and Duodenal Transcriptome Analysis of Chinese Beef Cattle With Divergent Feed Efficiency Using RNA-Seq","volume":"12","author":"C Yang","year":"2021","journal-title":"Front Genet"},{"issue":"7","key":"pone.0289939.ref003","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.2527\/jas.2009-2655","article-title":"Application of biological simulation models in estimating feed efficiency of finishing steers","volume":"88","author":"CB Williams","year":"2010","journal-title":"J Anim Sci"},{"issue":"5","key":"pone.0289939.ref004","doi-asserted-by":"crossref","first-page":"4041","DOI":"10.3168\/jds.2018-15504","article-title":"Effect of diet energy density and genomic residual feed intake on prebred dairy heifer feed efficiency, growth, and manure excretion","volume":"102","author":"KT Williams","year":"2019","journal-title":"J Dairy Sci"},{"issue":"1","key":"pone.0289939.ref005","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2527\/jas.2009-2196","article-title":"Effect of divergence in residual feed intake on feeding behavior, blood metabolic variables, and body composition traits in growing beef heifers","volume":"88","author":"AK Kelly","year":"2010","journal-title":"J Anim Sci"},{"issue":"10","key":"pone.0289939.ref006","first-page":"1648","article-title":"Grazed grass herbage intake and performance of beef heifers with predetermined phenotypic residual feed intake classification","volume":"6","author":"P Lawrence","year":"2012","journal-title":"AnimalEpub 2012"},{"issue":"10","key":"pone.0289939.ref007","doi-asserted-by":"crossref","first-page":"2382","DOI":"10.2527\/jas.2006-657","article-title":"Genetic and phenotypic relationships of feeding behavior and temperament with performance, feed efficiency, ultrasound, and carcass merit of beef cattle","volume":"85","author":"JD Nkrumah","year":"2007","journal-title":"J Anim Sci"},{"key":"pone.0289939.ref008","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.livsci.2018.06.013","article-title":"Genetic correlations and heritability estimates for dry matter intake, weight gain and feed efficiency of Nellore cattle in feedlot","volume":"214","author":"GHG Polizel","year":"2018","journal-title":"Livestock Science"},{"issue":"4","key":"pone.0289939.ref009","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1017\/S1751731119002544","article-title":"Genetic parameters for feed efficiency in Romane rams and responses to single-generation selection","volume":"14","author":"F Tortereau","year":"2020","journal-title":"Animal"},{"issue":"4","key":"pone.0289939.ref010","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.2527\/jas.2012-5862","article-title":"Cell Biology Symposium: genetics of feed efficiency in dairy and beef cattle","volume":"91","author":"DP Berry","year":"2013","journal-title":"J Anim Sci"},{"issue":"9","key":"pone.0289939.ref011","doi-asserted-by":"crossref","first-page":"3947","DOI":"10.1093\/jas\/skz241","article-title":"Effect of divergence in residual feed intake on expression of lipid metabolism-related genes in the liver of meat-type ducks","volume":"97","author":"S Jin","year":"2019","journal-title":"Journal of Animal Science"},{"key":"pone.0289939.ref012","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.ijbiomac.2021.11.202","article-title":"RNA-Seq reveals the potential molecular mechanisms of bovine KLF6 gene in the regulation of adipogenesis","volume":"195","author":"SHA Raza","year":"2022","journal-title":"Int J Biol Macromol"},{"key":"pone.0289939.ref013","doi-asserted-by":"crossref","first-page":"145934","DOI":"10.1016\/j.gene.2021.145934","article-title":"Identifying the key genes and functional enrichment pathways associated with feed efficiency in cattle","volume":"807","author":"C Yang","year":"2022","journal-title":"Gene"},{"key":"pone.0289939.ref014","doi-asserted-by":"crossref","first-page":"44345","DOI":"10.1038\/srep44345","article-title":"Corrigendum: Gene expression differences in Longissimus muscle of Nelore steers genetically divergent for residual feed intake","volume":"7","author":"PC Tizioto","year":"2017","journal-title":"Sci Rep"},{"issue":"11","key":"pone.0289939.ref015","doi-asserted-by":"crossref","first-page":"4764","DOI":"10.2527\/jas2016.1320","article-title":"Gene expression analysis of blood, liver, and muscle in cattle divergently selected for high and low residual feed intake","volume":"95","author":"M Khansefid","year":"2017","journal-title":"J Anim Sci"},{"issue":"1","key":"pone.0289939.ref016","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.bbrc.2015.07.092","article-title":"Gene expression profiling of hormonal regulation related to the residual feed intake of Holstein cattle","volume":"465","author":"YM Xi","year":"2015","journal-title":"Biochem Biophys Res Commun"},{"issue":"3","key":"pone.0289939.ref017","doi-asserted-by":"crossref","first-page":"e0152274","DOI":"10.1371\/journal.pone.0152274","article-title":"Identification of Gene Networks for Residual Feed Intake in Angus Cattle Using Genomic Prediction and RNA-seq","volume":"11","author":"KL Weber","year":"2016","journal-title":"Plos One"},{"key":"pone.0289939.ref018","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1186\/s12864-016-2935-4","article-title":"Transcriptome profiling of the rumen epithelium of beef cattle differing in residual feed intake","volume":"17","author":"RS Kong","year":"2016","journal-title":"BMC Genomics"},{"key":"pone.0289939.ref019","doi-asserted-by":"crossref","first-page":"1090517","DOI":"10.3389\/fvets.2023.1090517","article-title":"Multi-transcriptomics reveals RLMF axis-mediated signaling molecules associated with bovine feed efficiency","volume":"10","author":"C Yang","year":"2023","journal-title":"Front Vet Sci"},{"issue":"2","key":"pone.0289939.ref020","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.cmet.2006.06.008","article-title":"Carbohydrate response element binding protein, ChREBP, a transcription factor coupling hepatic glucose utilization and lipid synthesis","volume":"4","author":"K Uyeda","year":"2006","journal-title":"Cell Metab"},{"issue":"1","key":"pone.0289939.ref021","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s12864-018-5406-2","article-title":"Identification of a metabolomic signature associated with feed efficiency in beef cattle","volume":"20","author":"FJ Novais","year":"2019","journal-title":"BMC Genomics"},{"issue":"1","key":"pone.0289939.ref022","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s12864-021-07423-6","article-title":"Consensus module analysis of abdominal fat deposition across multiple broiler lines","volume":"22","author":"H Yuan","year":"2021","journal-title":"BMC Genomics"},{"issue":"6","key":"pone.0289939.ref023","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1080\/15476286.2020.1824060","article-title":"Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle","volume":"18","author":"HZ Sun","year":"2021","journal-title":"RNA Biol"},{"key":"pone.0289939.ref024","article-title":"fastqcr","author":"A. Kassambara","year":"2017","journal-title":"Quality Control of Sequencing Data"},{"key":"pone.0289939.ref025","author":"H Li","year":"2013","journal-title":"Sequence analysis The Sequence Alignment\/Map format and SAMtools."},{"issue":"9","key":"pone.0289939.ref026","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1038\/nprot.2016.095","article-title":"Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown","volume":"11","author":"M Pertea","year":"2016","journal-title":"Nat Protoc"},{"key":"pone.0289939.ref027","doi-asserted-by":"crossref","first-page":"616388","DOI":"10.3389\/fgene.2021.616388","article-title":"Transcriptome Characterization of Short Distance Transport Stress in Beef Cattle Blood","volume":"12","author":"H Zhao","year":"2021","journal-title":"Front Genet"},{"issue":"1","key":"pone.0289939.ref028","doi-asserted-by":"crossref","first-page":"17","DOI":"10.2202\/1544-6115.1128","article-title":"A general framework for weighted gene co-expression network analysis","volume":"4","author":"B Zhang","year":"2005","journal-title":"Stat Appl Genet Mol Biol"},{"issue":"S4","key":"pone.0289939.ref029","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1186\/1752-0509-8-S4-S11","article-title":"cytoHubba: identifying hub objects and sub-networks from complex interactome","volume":"84","author":"CH Chin","year":"2014","journal-title":"BMC Syst Biol"},{"issue":"1","key":"pone.0289939.ref030","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s12864-019-6362-1","article-title":"Genetic architecture of quantitative traits in beef cattle revealed by genome wide association studies of imputed whole genome sequence variants: I: feed efficiency and component traits","volume":"21","author":"F Zhang","year":"2020","journal-title":"BMC Genomics"},{"issue":"1\u20132","key":"pone.0289939.ref031","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s11010-018-3324-x","article-title":"Epigallocatechin gallate suppresses hepatic cholesterol synthesis by targeting SREBP-2 through SIRT1\/FOXO1 signaling pathway","volume":"448","author":"Y Li","year":"2018","journal-title":"Molecular and Cellular Biochemistry"},{"issue":"1","key":"pone.0289939.ref032","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1186\/s12859-018-2553-z","article-title":"Gene co-expression networks from RNA sequencing of dairy cattle identifies genes and pathways affecting feed efficiency","volume":"19","author":"SM Salleh","year":"2018","journal-title":"BMC Bioinformatics"},{"issue":"3","key":"pone.0289939.ref033","doi-asserted-by":"crossref","first-page":"e0152274","DOI":"10.1371\/journal.pone.0152274","article-title":"Identification of Gene Networks for Residual Feed Intake in Angus Cattle Using Genomic Prediction and RNA-seq","volume":"11","author":"KL Weber","year":"2016","journal-title":"Plos One"},{"issue":"11","key":"pone.0289939.ref034","doi-asserted-by":"crossref","first-page":"3615","DOI":"10.1534\/g3.116.032839","article-title":"Transcriptome Analysis Reveals that Vitamin A Metabolism in the Liver Affects Feed Efficiency in Pigs","volume":"6","author":"Y Zhao","year":"2016","journal-title":"G3-Genes Genomes Genetics"},{"issue":"1","key":"pone.0289939.ref035","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1186\/s12864-017-3639-0","article-title":"A transcriptome multi-tissue analysis identifies biological pathways and genes associated with variations in feed efficiency of growing pigs","volume":"18","author":"F Gondret","year":"2017","journal-title":"BMC Genomics"},{"issue":"4","key":"pone.0289939.ref036","doi-asserted-by":"crossref","first-page":"861","DOI":"10.2527\/2006.844861x","article-title":"The relationship between mitochondrial function and residual feed intake in Angus steers","volume":"84","author":"WH Kolath","year":"2006","journal-title":"Journal of Animal Science"},{"issue":"1","key":"pone.0289939.ref037","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1093\/ps\/85.1.8","article-title":"Feed efficiency and mitochondrial function","volume":"85","author":"W Bottje","year":"2006","journal-title":"Poult Sci"},{"issue":"4","key":"pone.0289939.ref038","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.2527\/jas.2012-5787","article-title":"Cell Biology Symposium: feed efficiency: mitochondrial function to global gene expression","volume":"91","author":"W Bottje","year":"2013","journal-title":"J Anim Sci"},{"issue":"7","key":"pone.0289939.ref039","doi-asserted-by":"crossref","first-page":"3134","DOI":"10.2527\/jas.2013-7409","article-title":"Relationships between residual feed intake and hepatic mitochondrial function in growing beef cattle","volume":"92","author":"PA Lancaster","year":"2014","journal-title":"Journal of Animal Science"},{"issue":"1","key":"pone.0289939.ref040","article-title":"A Transcriptome Analysis Reveals that Hepatic Glycolysis and Lipid Synthesis Are Negatively Associated with Feed Efficiency in DLY Pigs","volume":"10","author":"C Xu","year":"2020","journal-title":"Scientific Reports"},{"issue":"1","key":"pone.0289939.ref041","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1186\/s12864-020-6713-y","article-title":"Identification of key genes and pathways associated with feed efficiency of native chickens based on transcriptome data via bioinformatics analysis","volume":"21","author":"L Yang","year":"2020","journal-title":"BMC Genomics"},{"issue":"7","key":"pone.0289939.ref042","doi-asserted-by":"crossref","first-page":"2454","DOI":"10.3382\/ps\/pew453","article-title":"Enhanced expression of proteins involved in energy production and transfer in breast muscle of pedigree male broilers exhibiting high feed efficiency","volume":"96","author":"WG Bottje","year":"2017","journal-title":"Poult Sci"},{"key":"pone.0289939.ref043","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3389\/fgene.2019.00117","article-title":"RNA-Seq of Liver From Pigs Divergent in Feed Efficiency Highlights Shifts in Macronutrient Metabolism, Hepatic Growth and Immune Response","volume":"10","author":"J Horodyska","year":"2019","journal-title":"Front Genet"},{"issue":"1","key":"pone.0289939.ref044","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s40104-015-0031-2","article-title":"A review of feed efficiency in swine: biology and application","volume":"6","author":"JF Patience","year":"2015","journal-title":"J Anim Sci Biotechnol"},{"issue":"7","key":"pone.0289939.ref045","doi-asserted-by":"crossref","first-page":"3331","DOI":"10.2527\/jas.2015-8975","article-title":"Transcriptomic analysis by RNA sequencing reveals that hepatic interferon-induced genes may be associated with feed efficiency in beef heifers","volume":"93","author":"F Paradis","year":"2015","journal-title":"J Anim Sci"},{"issue":"1","key":"pone.0289939.ref046","doi-asserted-by":"crossref","first-page":"7303","DOI":"10.1038\/s41598-018-25605-3","article-title":"Transcriptome analyses reveal reduced hepatic lipid synthesis and accumulation in more feed efficient beef cattle","volume":"8","author":"R Mukiibi","year":"2018","journal-title":"Sci Rep"},{"key":"pone.0289939.ref047","doi-asserted-by":"crossref","first-page":"32953","DOI":"10.1038\/srep32953","article-title":"Bamboo shoot fiber prevents obesity in mice by modulating the gut microbiota","volume":"6","author":"X Li","year":"2016","journal-title":"Sci Rep"},{"issue":"1","key":"pone.0289939.ref048","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1186\/s12879-017-2340-x","article-title":"Genetic variation in the microsomal triglyceride transfer protein (-493G\/T) is associated with hepatic steatosis in patients infected with hepatitis C virus","volume":"17","author":"MC Magri","year":"2017","journal-title":"BMC Infect Dis"},{"issue":"7","key":"pone.0289939.ref049","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1016\/j.celrep.2017.04.064","article-title":"Lack of MTTP Activity in Pluripotent Stem Cell-Derived Hepatocytes and Cardiomyocytes Abolishes apoB Secretion and Increases Cell Stress","volume":"19","author":"Y Liu","year":"2017","journal-title":"Cell Rep"},{"issue":"6","key":"pone.0289939.ref050","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.3892\/etm.2011.328","article-title":"Effects of insulin resistance and hepatic lipid accumulation on hepatic mRNA expression levels of apoB, MTP and L-FABP in non-alcoholic fatty liver disease","volume":"2","author":"N Higuchi","year":"2011","journal-title":"Exp Ther Med"},{"issue":"1","key":"pone.0289939.ref051","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1186\/s12933-018-0785-x","article-title":"The negative effect of ANGPTL8 on HDL-mediated cholesterol efflux capacity","volume":"17","author":"M Luo","year":"2018","journal-title":"Cardiovasc DiabetolPubMed Central PMCID"},{"issue":"8","key":"pone.0289939.ref052","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1093\/carcin\/bgx058","article-title":"Cholesterol uptake and regulation in high-grade and lethal prostate cancers","volume":"38","author":"KH Stopsack","year":"2017","journal-title":"Carcinogenesis"},{"issue":"1","key":"pone.0289939.ref053","doi-asserted-by":"crossref","first-page":"e009146","DOI":"10.1161\/JAHA.118.009146","article-title":"Liver X Receptor\/Retinoid X Receptor Pathway Plays a Regulatory Role in Pacing-Induced Cardiomyopathy","volume":"8","author":"YS Lin","year":"2019","journal-title":"J Am Heart Assoc"},{"key":"pone.0289939.ref054","doi-asserted-by":"crossref","first-page":"16984","DOI":"10.1038\/srep16984","article-title":"Lipidomic Profiling of Liver Tissue from Obesity-Prone and Obesity-Resistant Mice Fed a High Fat Diet","volume":"5","author":"M Nam","year":"2015","journal-title":"Sci Rep"},{"issue":"42","key":"pone.0289939.ref055","doi-asserted-by":"crossref","first-page":"30432","DOI":"10.1074\/jbc.M113.501247","article-title":"Lipid absorption defects in intestine-specific microsomal triglyceride transfer protein and ATP-binding cassette transporter A1-deficient mice","volume":"288","author":"J Iqbal","year":"2013","journal-title":"J Biol Chem"},{"key":"pone.0289939.ref056","doi-asserted-by":"crossref","first-page":"112677","DOI":"10.1016\/j.biopha.2022.112677","article-title":"LCAT- targeted therapies: Progress, failures and future","volume":"147","author":"K Yang","year":"2022","journal-title":"Biomed Pharmacother"},{"issue":"2","key":"pone.0289939.ref057","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1097\/MOL.0000000000000486","article-title":"Novel metabolic phenotypes in lecithin cholesterol acyltyransferase-deficient mice","volume":"29","author":"DS Ng","year":"2018","journal-title":"Curr Opin Lipidol"},{"issue":"1","key":"pone.0289939.ref058","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1186\/s12864-017-3639-0","article-title":"A transcriptome multi-tissue analysis identifies biological pathways and genes associated with variations in feed efficiency of growing pigs","volume":"18","author":"F Gondret","year":"2017","journal-title":"BMC Genomics"},{"issue":"7","key":"pone.0289939.ref059","doi-asserted-by":"crossref","first-page":"7942","DOI":"10.1096\/fj.201802704R","article-title":"Estrogen-related receptor gamma regulates hepatic triglyceride metabolism through phospholipase A2 G12B","volume":"33","author":"L Chen","year":"2019","journal-title":"FASEB J"},{"key":"pone.0289939.ref060","doi-asserted-by":"crossref","first-page":"8686248","DOI":"10.1155\/2020\/8686248","article-title":"Quercetin Improving Lipid Metabolism by Regulating Lipid Metabolism Pathway of Ileum Mucosa in Broilers","volume":"2020","author":"M Wang","year":"2020","journal-title":"Oxid Med Cell Longev"},{"issue":"7","key":"pone.0289939.ref061","doi-asserted-by":"crossref","first-page":"e21737","DOI":"10.1096\/fj.202002620RR","article-title":"Relaxin has beneficial effects on liver lipidome and metabolic enzymes","volume":"35","author":"A Aragon-Herrera","year":"2021","journal-title":"FASEB J"},{"issue":"1","key":"pone.0289939.ref062","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s12944-017-0459-9","article-title":"A Single Nucleotide Polymorphism in the FADS1 Gene is Associated with Plasma Fatty Acid and Lipid Profiles and Might Explain Gender Difference in Body Fat Distribution","volume":"16","author":"H Guo","year":"2017","journal-title":"Lipids Health Dis"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0289939","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T13:35:05Z","timestamp":1695821705000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0289939"}},"subtitle":[],"editor":[{"given":"Muhammad Sajid","family":"Arshad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,9,27]]},"references-count":62,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,9,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0289939","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,27]]}}}