{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:19:19Z","timestamp":1747196359283,"version":"3.40.5"},"reference-count":100,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T00:00:00Z","timestamp":1692230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institute for the Promotion of Argentine Beef","award":["\u201cCharacterization of risk and mitigation of impact of STEC in the beef chain\u201d."],"award-info":[{"award-number":["\u201cCharacterization of risk and mitigation of impact of STEC in the beef chain\u201d."]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The aim of this study was to perform a quantitative microbial risk assessment (QMRA) of Shiga toxin-producing<jats:italic>Escherichia coli<\/jats:italic>hemolytic uremic syndrome (STEC-HUS) linked to the consumption of Kosher beef produced in Argentina and consumed in Israel in children under 14 years. A probabilistic risk assessment model was developed to characterize STEC prevalence and contamination levels in the beef supply chain (cattle primary production, cattle transport, processing and storage in the abattoir, for export and at retail, and home preparation and consumption). The model was implemented in Microsoft Excel 2016 with the @Risk add-on package. Results of 302 surveys with data collected in Israel were as follows: 92.3% of people consumed beef, mostly at home, and 98.2% preferred levels of cooking that ensured STEC removal from the surface of beef cuts. The preferred degree of ground beef doneness was \u201cwell-done\u201d (48.2%). Cooking preference ranged from red to \u201cmedium-well done\u201d (51.8%). Median HUS probability from Argentinean beef cut and ground beef consumption in children under 14 years old was<jats:italic>&lt;<\/jats:italic>10<jats:sup>\u221215<\/jats:sup>and 8.57x10<jats:sup>-10<\/jats:sup>, respectively. The expected average annual number of HUS cases and deaths due to beef cut and ground beef consumption was zero. Risk of infection and HUS probability correlated with salting effect on<jats:italic>E<\/jats:italic>.<jats:italic>coli<\/jats:italic>count, processing raw beef before vegetables, ways of storage and refrigeration temperature at home, joint consumption of salad and beef cuts, degree of beef doneness and cutting board washing with detergent after each use with beef and vegetables. The STEC-HUS risk in Israel from consumption of bovine beef produced in Argentina was negligible. The current QMRA results were similar to those of previous beef cut consumption QMRA in Argentina and lower than any of the QMRA performed worldwide in other STEC-HUS linked to ground beef consumption. This study confirms the importance of QMRA to estimate and manage the risk of STEC-HUS from beef consumption. The impact variables identified in the sensitivity analysis allowed us to optimize resources and time management, to focus on accurate actions and to avoid taking measures that would not have an impact on the risk of STEC-HUS.<\/jats:p>","DOI":"10.1371\/journal.pone.0290182","type":"journal-article","created":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T17:36:44Z","timestamp":1692293804000},"page":"e0290182","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantitative microbial risk assessment of haemolytic uremic syndrome associated with Argentinean kosher beef consumption in Israel"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4823-4116","authenticated-orcid":true,"given":"Victoria","family":"Brusa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Dolev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6537-8782","authenticated-orcid":true,"given":"Marcelo","family":"Signorini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8707-8932","authenticated-orcid":true,"given":"Gerardo","family":"Leotta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,8,17]]},"reference":[{"issue":"3\u20134","key":"pone.0290182.ref001","first-page":"11","article-title":"Verocytotoxin-producing Escherichia coli (VTEC)","volume":"140","author":"MA Karmali","year":"2010","journal-title":"Vet Microbiol"},{"issue":"1","key":"pone.0290182.ref002","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s00467-015-3076-8","article-title":"An international consensus approach to the management of atypical hemolytic uremic syndrome in children","volume":"31","author":"C Loirat","year":"2016","journal-title":"Pediatr Nephrol"},{"issue":"3","key":"pone.0290182.ref003","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1159\/000505401","article-title":"Hemolytic Uremic Syndrome: A Contemporary Pediatric Experience","volume":"144","author":"H Alfandary","year":"2020","journal-title":"Nephron"},{"key":"pone.0290182.ref004","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1186\/s12882-022-02746-2","article-title":"Incidence and cost of haemolytic uraemic syndrome in urban China: a national population-based analysis","volume":"23","author":"J Feng","year":"2022","journal-title":"BMC Nephrology"},{"key":"pone.0290182.ref005","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-45092-6","volume-title":"Escherichia coli in the Americas","author":"AG Torres","year":"2016","edition":"1"},{"unstructured":"\u00c1rea de Vigilancia de la Salud de la Direcci\u00f3n de Epidemiolog\u00eda. Ministerio de Salud de la Naci\u00f3n. Bolet\u00edn Integrado de Vigilancia N\u00b0 611 \u2013SE 29\u20132022. Ciudad Aut\u00f3noma de Buenos Aires 2022.","key":"pone.0290182.ref006"},{"unstructured":"Minstry for Primary Industries. New Zealand Government. Annual report concerning Foodborne Diseases in New Zealand 2020. New Zealand Food Safety Technical PaperNo: 2021\/16. ISBN No: 978-1-99-101934-9 (online) ISSN No: 2624-022X (online). 2021","key":"pone.0290182.ref007"},{"unstructured":"ECDC. Shiga toxin-producing Escherichia coli (STEC) infection. In: ECDC. Annual epidemiological report for 2019. Stockholm: ECDC; 2021.","key":"pone.0290182.ref008"},{"key":"pone.0290182.ref009","first-page":"41","article-title":"Haemolytic uremic syndrome surveillance in children less than 15 years in Belgium, 2009\u20132015","volume":"76","author":"S Jacquinet","journal-title":"Public Health. 2018"},{"unstructured":"Center for Disease Control. FoodNet Fast. Foodborne Diseases Active Surveillance Network (FoodNet). HUS. 2022 [cited 2022 Aug 9]. Available from: https:\/\/wwwn.cdc.gov\/foodnetfast\/HUS.","key":"pone.0290182.ref010"},{"key":"pone.0290182.ref011","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.ijfoodmicro.2008.12.031","article-title":"Analysis of foodborne outbreak data reported internationally for source attribution","volume":"130","author":"JD Greig","year":"2009","journal-title":"Int. J. Food Microbiol"},{"key":"pone.0290182.ref012","first-page":"3138","article-title":"Scientific Opinion on VTEC-seropathotype and scientific criteria regarding pathogenicity assessment","volume":"11","author":"EFSA-BIOHAZ","year":"2013","journal-title":"EFSA J"},{"issue":"e236","key":"pone.0290182.ref013","first-page":"9","article-title":"Global and regional source attribution of Shiga toxin-producing Escherichia coli infections using analysis of outbreak surveillance data","volume":"147","author":"SM Pires","year":"2019","journal-title":"Epidemiol Infect"},{"key":"pone.0290182.ref014","doi-asserted-by":"crossref","first-page":"e235","DOI":"10.1017\/S0950268819001183","article-title":"Associating sporadic, foodborne illness caused by Shiga toxin-producing Escherichia coli with specific foods: a systematic review and meta-analysis of case-control studies","volume":"147","author":"B Devleesschauwer","year":"2019","journal-title":"Epidemiol Infect"},{"issue":"11","key":"pone.0290182.ref015","doi-asserted-by":"crossref","first-page":"9","DOI":"10.4315\/0362-028X.JFP-19-014","article-title":"Outbreaks of Shiga Toxin\u2013Producing Escherichia coli Linked to Sprouted Seeds, Salad, and Leafy Greens: A Systematic Review","volume":"82","author":"E Kintz","year":"2019","journal-title":"J Food Prot"},{"key":"pone.0290182.ref016","doi-asserted-by":"crossref","first-page":"e8","DOI":"10.1111\/zph.12403","article-title":"Attribution of human infections with Shiga toxin-producing Escherichia coli (STEC) to livestock sources and identification of source-specific risk factors, The Netherlands (2010\u20132014)","volume":"65","author":"L Mughini-Gras","journal-title":"Zoonoses Public Health. 2018"},{"key":"pone.0290182.ref017","doi-asserted-by":"crossref","first-page":"e0183641","DOI":"10.1371\/journal.pone.0183641","article-title":"Attribution of global foodborne disease to specific foods: Findings from a World Health Organization structured expert elicitation","volume":"12","author":"S Hoffmann","year":"2017","journal-title":"PLoS One"},{"key":"pone.0290182.ref018","doi-asserted-by":"crossref","first-page":"e100117","DOI":"10.1016\/j.mran.2020.100117","article-title":"Risk factors for sporadic infections caused by Shiga toxin-producing Escherichia coli: a systematic review and meta-analysis","volume":"17","author":"JC Augustin","year":"2021","journal-title":"Microb Risk Anal"},{"issue":"2","key":"pone.0290182.ref019","first-page":"16","article-title":"Colonization of beef cattle by Shiga toxin-producing Escherichia coli during the first year of life: a cohort study","volume":"11","author":"RA Mir","year":"2016","journal-title":"PLoS One"},{"key":"pone.0290182.ref020","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1111\/jam.13677","article-title":"Low prevalence of the \u2018gang of seven\u2019 and absence of the O80:H2 serotypes among Shigatoxigenic and enteropathogenic Escherichia coli (STEC and EPEC) in intestinal contents of healthy cattle at two slaughterhouses in Belgium in 2014","volume":"124","author":"D Thiry","year":"2017","journal-title":"J Appl Microbiol"},{"issue":"2","key":"pone.0290182.ref021","first-page":"131","article-title":"Molecular characterization of Shiga toxin producing Escherichia coli isolated from 2 livestock establishments of Paraguay","volume":"52","author":"SM Rivelli Zea","year":"2020","journal-title":"Rev Argent Microbiol"},{"issue":"11","key":"pone.0290182.ref022","doi-asserted-by":"crossref","first-page":"e0242317","DOI":"10.1371\/journal.pone.0242317","article-title":"Quantitative risk assessment of haemolytic uremic syndrome associated with beef consumption in Argentina","volume":"15","author":"V Brusa","year":"2020","journal-title":"PLoS One"},{"key":"pone.0290182.ref023","doi-asserted-by":"crossref","first-page":"105133","DOI":"10.1016\/j.prevetmed.2020.105133","article-title":"Characterization and molecular subtyping of Shiga toxin\u2013producing Escherichia coli strains in provincial abattoirs from the Province of Buenos Aires, Argentina, during 2016\u20132018","volume":"183","author":"M Costa","journal-title":"Prev Vet Med. 2020"},{"issue":"2","key":"pone.0290182.ref024","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.meatsci.2010.05.027","article-title":"Occurrence of Shiga toxin-producing E. coli (STEC) on carcasses and retail beef cuts in the marketing chain of beef in Argentina","volume":"86","author":"AI Etcheverria","year":"2010","journal-title":"Meat Sci"},{"key":"pone.0290182.ref025","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.fm.2012.06.002","article-title":"Serotypes and virulotypes of non-O157 shiga-toxin producing Escherichia coli (STEC) on bovine hides and carcasses","volume":"32","author":"\u00c1 Monaghan","year":"2012","journal-title":"Food Microbiol"},{"key":"pone.0290182.ref026","article-title":"Aislamiento y caracterizaci\u00f3n de cepas de Escherichia coli productor de toxina Shiga desde carne de vacuno nacional e importada, distribu\u00edda en los principales supermercados de la provincia de Santiago","author":"CB Baeza Quiroz","year":"2013","journal-title":"Chile: Escuela de Salud P\u00fablica. Universidad Mayor"},{"issue":"8","key":"pone.0290182.ref027","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1371\/journal.pone.0183248","article-title":"Isolation and characterization of non-O157 Shiga toxin-producing Escherichia coli from beef carcasses, cuts and trimmings of abattoirs in Argentina","volume":"12","author":"V Brusa","year":"2017","journal-title":"PLoS One"},{"issue":"1","key":"pone.0290182.ref028","first-page":"32","article-title":"Frequency, characterization and genotypic analysis of Shiga toxin-producing Escherichia coli in beef slaughterhouses of Argentina","volume":"51","author":"M Cap","year":"2019","journal-title":"Rev Argent Microbiol"},{"issue":"126","key":"pone.0290182.ref029","first-page":"12","article-title":"Presence of Shiga toxin-producing Escherichia coli (STEC) in fresh beef marketed in 13 eegions of ITALY (2017)","volume":"6","author":"BM Varcasia","year":"2018","journal-title":"Microorganisms"},{"key":"pone.0290182.ref030","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.ijfoodmicro.2008.05.040","article-title":"Quantitative risk assessment for Escherichia coli O157:H7 in frozen ground beef patties consumed by young children in French households","volume":"128","author":"ML Delignette-Muller","year":"2008","journal-title":"Int. J. Food Microbiol"},{"unstructured":"Duffy G, O\u2019 Brien S, Carney E, Butler F, Cummins E, Nally P, et al. A quantitative risk assessment of E. coli O157:H7 in Irish minced beef. In: Teagasc, editor. Ireland2005.","key":"pone.0290182.ref031"},{"issue":"9","key":"pone.0290182.ref032","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.4315\/0362-028X-67.9.1991","article-title":"Draft risk assessment of the public health impact of Escherichia coli O157:H7 in ground beef","volume":"67","author":"E Ebel","year":"2004","journal-title":"J Food Prot"},{"issue":"2\u20133","key":"pone.0290182.ref033","first-page":"9","article-title":"Quantitative risk assessment for verocytotoxigenic Escherichia coli in ground beef hamburgers in Argentina","volume":"132","author":"M Signorini","year":"2009","journal-title":"Int J Food Microbiol"},{"key":"pone.0290182.ref034","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.meatsci.2013.04.040","article-title":"Microbial pathogen control in the beef chain: recent research advances","volume":"97","author":"S Buncic","year":"2014","journal-title":"Meat Sci"},{"issue":"3","key":"pone.0290182.ref035","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.biosystemseng.2011.03.002","article-title":"Use of fluorescence imaging as a hygiene indicator for beef and lamb carcasses in UK slaughterhouses","volume":"109","author":"D Burfoot","year":"2011","journal-title":"Biosyst. Eng"},{"issue":"3","key":"pone.0290182.ref036","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.meatsci.2014.06.026","article-title":"Pre- and Post-Harvest Interventions to Reduce Pathogen Contamination in the U.S. Beef Industry","volume":"98","author":"TL Wheeler","year":"2014","journal-title":"Meat Sci"},{"issue":"3","key":"pone.0290182.ref037","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.meatsci.2014.05.021","article-title":"Halal and kosher slaughter methods and meat quality: a review","volume":"98","author":"MM Farouk","year":"2014","journal-title":"Meat Sci"},{"key":"pone.0290182.ref038","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.3390\/pr9081381","article-title":"Conventional versus Ritual Slaughter\u2013Ethical Aspects and Meat Quality","volume":"9","author":"J Zurek","year":"2021","journal-title":"Processes"},{"key":"pone.0290182.ref039","first-page":"1066","article-title":"Escherichia coli productor de toxina Shiga","author":"V Brusa","year":"2019","journal-title":"Microbiolog\u00eda Veterinaria"},{"unstructured":"Ministry for Primary Industries. Shiga toxin-producing Escherichia coli. New Zealand Food Safety. ESR Science for Communities. 2018. Available from: https:\/\/www.mpi.govt.nz\/dmsdocument\/26030-Shiga-toxin-producing-Escherichia-coli-STEC","key":"pone.0290182.ref040"},{"issue":"1","key":"pone.0290182.ref041","first-page":"7745","article-title":"Scientific Opinion on the microbiological safety of aged meat","volume":"21","author":"EFSA-BIOHAZ","year":"2023","journal-title":"EFSA J"},{"key":"pone.0290182.ref042","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.fm.2016.08.003","article-title":"The microbiology of beef carcasses and primals during chilling and commercial storage","volume":"61","author":"R Reid","year":"2017","journal-title":"Food Microbiol"},{"volume-title":"Detection and isolation of Shiga toxin-producing Escherichia coli and microbial population counts on Argentinean Kosher beef for export to Israel","author":"V Brusa","doi-asserted-by":"crossref","key":"pone.0290182.ref043","DOI":"10.24215\/15142590e071"},{"unstructured":"\u00c1rea de Vigilancia de la Salud de la Direcci\u00f3n de Epidemiolog\u00eda. Ministerio de Salud de la Naci\u00f3n Argentina. Bolet\u00edn Integrado de Vigilancia. N\u02da 439\u2014SE 06\u20132018. Ciudad Aut\u00f3noma de Buenos Aires2018.","key":"pone.0290182.ref044"},{"key":"pone.0290182.ref045","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1017\/S0950268807008771","article-title":"Hierarchical dose response of E. coli O157:H7 from human outbreaks incorporating heterogeneity in exposure","volume":"136","author":"PF Teunis","year":"2008","journal-title":"Epidemiol Infect"},{"issue":"6","key":"pone.0290182.ref046","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1089\/fpd.2013.1704","article-title":"Global incidence of human Shiga toxin-producing Escherichia coli infections and deaths: a systematic review and knowledge synthesis","volume":"11","author":"SE Majowicz","year":"2014","journal-title":"Foodborne Pathog Dis"},{"issue":"2","key":"pone.0290182.ref047","doi-asserted-by":"crossref","first-page":"4","DOI":"10.4315\/0362-028X-65.2.280","article-title":"Effects of the transportation of beef cattle from the feedyard to the packing plant on prevalence levels of Escherichia coli O157 and Salmonella spp","volume":"65","author":"RA Barham","year":"2002","journal-title":"J Food Prot"},{"issue":"2","key":"pone.0290182.ref048","doi-asserted-by":"crossref","first-page":"7","DOI":"10.4315\/0362-028X-70.2.280","article-title":"Transportation and lairage environment effects on prevalence, numbers, and diversity of Escherichia coli O157:H7 on hides and carcasses of beef cattle at processing","volume":"70","author":"TM Arthur","year":"2007","journal-title":"J Food Prot"},{"issue":"10","key":"pone.0290182.ref049","doi-asserted-by":"crossref","first-page":"6","DOI":"10.4315\/0362-028X.JFP-11-154","article-title":"Effects of long- or short-haul transportation of slaughter heifers and cattle liner microclimate on hide contamination with Escherichia coli O157","volume":"74","author":"K Stanford","year":"2011","journal-title":"J Food Prot"},{"issue":"6","key":"pone.0290182.ref050","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4315\/0362-028X-71.6.1114","article-title":"Impact of transportation and lairage on hide contamination with Escherichia coli O157 in finished beef cattle","volume":"71","author":"GA Dewell","year":"2008","journal-title":"J Food Prot"},{"issue":"9","key":"pone.0290182.ref051","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1089\/fpd.2009.0338","article-title":"The effects of transport and lairage on counts of Escherichia coli O157 in the feces and on the hides of individual cattle","volume":"6","author":"N Fegan","year":"2009","journal-title":"Foodborne Pathog Dis"},{"issue":"9","key":"pone.0290182.ref052","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1017\/S0950268818000973","article-title":"The effect of transportation and lairage on faecal shedding and carcass contamination with Escherichia coli O157 and O26 in very young calves in New Zealand","volume":"146","author":"P Jaros","year":"2018","journal-title":"Epidemiol Infect"},{"key":"pone.0290182.ref053","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1046\/j.1439-0450.2003.00674.x","article-title":"An Investigation on the effect of transport and lairage on the faecal shedding prevalence of Escherichia coli O157 in cattle","volume":"50","author":"D Minihan","year":"2003","journal-title":"J Vet Med B"},{"issue":"1","key":"pone.0290182.ref054","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4315\/0362-028X-70.1.17","article-title":"Impact of transportation of feedlot cattle to the harvest facility on the Prevalence of Escherichia","volume":"70","author":"AL Reicks","year":"2007","journal-title":"J Food Prot"},{"key":"pone.0290182.ref055","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.meatsci.2018.04.009","article-title":"Evaluation of decontamination efficacy of commonly used antimicrobial interventions for beef carcasses against Shiga toxin-producing Escherichia coli","volume":"142","author":"M Signorini","year":"2018","journal-title":"Meat Sci"},{"issue":"4","key":"pone.0290182.ref056","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1750-3841.2012.02647.x","article-title":"Mathematical modeling of growth of non-O157 Shiga toxin-producing Escherichia coli in raw ground beef","volume":"77","author":"L Huang","year":"2012","journal-title":"J Food Sci"},{"key":"pone.0290182.ref057","first-page":"9","article-title":"Quantitative risk model for verocytotoxigenic Escherichia coli cross-contamination during homemade hamburger preparation","volume":"41","author":"ML Signorini","year":"2009","journal-title":"Rev Argent Microbiol"},{"issue":"4","key":"pone.0290182.ref058","doi-asserted-by":"crossref","first-page":"9","DOI":"10.4315\/0362-028X.JFP-14-527","article-title":"Analysis of older adults\u2019 domestic kitchen storage practices in the United Kingdom: identification of risk factors associated with listeriosis","volume":"78","author":"EW Evans","year":"2015","journal-title":"J Food Prot"},{"issue":"1","key":"pone.0290182.ref059","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/1541-4337.12242","article-title":"The Use and Performance of Household Refrigerators: A Review","volume":"16","author":"C James","year":"2017","journal-title":"Compr Rev Food Sci F"},{"issue":"12","key":"pone.0290182.ref060","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1128\/AEM.69.12.7188-7193.2003","article-title":"Inoculum size influences bacterial cross contamination between surfaces","volume":"69","author":"R Montville","year":"2003","journal-title":"Appl Environ Microbiol"},{"key":"pone.0290182.ref061","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.lwt.2019.02.074","article-title":"Microbiological risk characterization in butcher shops from the province of Neuquen, Patagonia Argentina","volume":"107","author":"PA Barril","year":"2019","journal-title":"LWT"},{"key":"pone.0290182.ref062","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1155\/2014\/795104","article-title":"Characterization of Shiga toxin-producing Escherichia coli isolated from ground beef collected in different socioeconomic strata markets in Buenos Aires, Argentina","volume":"2014","author":"P Llorente","year":"2014","journal-title":"BioMed Res Internat"},{"key":"pone.0290182.ref063","doi-asserted-by":"crossref","first-page":"384","DOI":"10.3389\/fmicb.2015.00384","article-title":"Molecular epidemiology and genetic diversity of Listeria monocytogenes isolates from a wide variety of ready-to-eat foods and their relationship to clinical strains from listeriosis outbreaks in Chile","volume":"6","author":"D Montero","year":"2015","journal-title":"Front Microbiol"},{"issue":"5","key":"pone.0290182.ref064","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/jfs.12488","article-title":"Characterization of Shiga-toxin producing Escherichia coli isolated from meat products sold in San Luis, Argentina","volume":"38","author":"AG Salinas Ib\u00e1\u00f1ez","year":"2018","journal-title":"J Food Saf"},{"key":"pone.0290182.ref065","article-title":"Carnicer\u00edas saludables: detecci\u00f3n de Escherichia coli O157:H7 y no-O157:H7 en carne picada fresca y en instalaciones de comercios minoristas","author":"MJ Ruiz","year":"2013","journal-title":"XIII Congreso Argentino de Microbiolog\u00eda II Congreso de Microbiolog\u00eda Agr\u00edcola y Ambiental; Ciudad Aut\u00f3noma de Buenos Aires, Argentina"},{"issue":"1","key":"pone.0290182.ref066","doi-asserted-by":"crossref","first-page":"9","DOI":"10.4315\/0362-028X-64.1.72","article-title":"Quantification and variability analysis of bacterial cross-contamination rates in common food service tasks","volume":"64","author":"Y Chen","year":"2001","journal-title":"J Food Prot"},{"key":"pone.0290182.ref067","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1745-4565.1991.tb00078.x","article-title":"Estimating deppths of bacterial penetration into post-rigor carcass tissue during washing","volume":"12","author":"ME Anderson","year":"1991","journal-title":"J Food Saf"},{"unstructured":"Sporing SB. Escherichia coli O157:H7 risk assessment for production and cooking of blade tenderized beef steaks. M.Sc. Thesis, Kansas State University. 1996. Available from: https:\/\/corpora.tika.apache.org\/base\/docs\/govdocs1\/257\/257646.pdf.","key":"pone.0290182.ref068"},{"key":"pone.0290182.ref069","article-title":"Thermal inactivation of Escherichia coli 0157:H7","author":"SC Stringer","year":"2000","journal-title":"Journal of Applied Microbiology Symposium Supplement"},{"key":"pone.0290182.ref070","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4315\/0362-028X-59.3.230","article-title":"Heat resistance of Escherichia coli O157:H7 in a nutrient medium and in ground beef patties as influenced by storage and holding temperatures","volume":"59","author":"TC Jackson","year":"1996","journal-title":"J Food Prot"},{"key":"pone.0290182.ref071","article-title":"Thermal destruction of Escherichia coli 0157:H7 in hamburger","author":"VK Juneja","year":"1999","journal-title":"J Food Prot"},{"unstructured":"Ministerio de Salud. Encuesta Nacional de Nutrici\u00f3n y Salud 2004\/5. Argentina2012. p. 54.","key":"pone.0290182.ref072"},{"unstructured":"World Bank. 2022 [cited 2022 Aug 9]. Available from: https:\/\/datos.bancomundial.org\/indicator\/SP.POP.0014.TO.ZS?locations=IL","key":"pone.0290182.ref073"},{"unstructured":"IPCVA. [2019] [cited 2022 Aug 9]. Available from: http:\/\/www.ipcva.com.ar\/.","key":"pone.0290182.ref074"},{"key":"pone.0290182.ref075","first-page":"9","article-title":"Preferred reporting items for systematic review and meta-analysis protocols (PRISMA-P) 2015 statement","volume":"4","author":"D Moher","journal-title":"Syst Rev. 2015"},{"issue":"2","key":"pone.0290182.ref076","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.foodcont.2012.03.003","article-title":"A risk assessment model for Escherichia coli O157:H7 in ground beef and beef cuts in Canada: Evaluating the effects of interventions","volume":"29","author":"BA Smith","year":"2013","journal-title":"Food Control"},{"key":"pone.0290182.ref077","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0168-1605(98)00028-2","article-title":"Quantitative risk assessment for Escherichia coli O157:H7 in ground beef hamburgers","volume":"41","author":"MH Cassin","year":"1998","journal-title":"Int J Food Microbiol"},{"key":"pone.0290182.ref078","article-title":"Shiga-toxinproducing E. coli in ground beef manufactured from Australian beef: Process improvement. Australia: Meat and Livestock","author":"AM Lammerding","year":"1999","journal-title":"Australia."},{"key":"pone.0290182.ref079","first-page":"169","volume-title":"Risk assessment of Shiga-toxin producing Escherichia coli O157 in steak tartare in the Netherlands. In: Environment NIfPHat","author":"MJ Nauta","year":"2001"},{"issue":"1","key":"pone.0290182.ref080","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/risa.12248","article-title":"Risk assessment of Escherichia coli O157 illness from consumption of hamburgers in the United States made from Australian manufacturing beef","volume":"35","author":"A Kiermeier","year":"2015","journal-title":"Risk Anal"},{"volume-title":"Escherichia coli O157:H7 in ground beef: Review of a draft risk assessment. In: Board BoHPaDPFaN","year":"2002","key":"pone.0290182.ref081"},{"doi-asserted-by":"crossref","unstructured":"EFSA-BIOHAZ. Scientific Opinion on the public health risks related to the maintenance of the cold chain during storage and transport of meat. Part 2 (minced meat from all species). EFSA J. 2014; 12 (7):30. doi: 10.2903\/j.efsa.2014.3783","key":"pone.0290182.ref082","DOI":"10.2903\/j.efsa.2014.3783"},{"issue":"1","key":"pone.0290182.ref083","first-page":"13","article-title":"A review of quantitative microbial risk assessment in the management of Escherichia coli O157:H7 on beef","volume":"74","author":"G Duffy","year":"2006","journal-title":"Meat Sci. 2006"},{"issue":"3","key":"pone.0290182.ref084","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.ijfoodmicro.2007.05.004","article-title":"Survey of domestic food handling practices in New Zealand","volume":"117","author":"SE Gilbert","year":"2007","journal-title":"Int. J. Food Microbiol"},{"issue":"7","key":"pone.0290182.ref085","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4315\/0362-028X-66.7.1176","article-title":"Cross-contamination of Lettuce (Lactuca sativa L.) with Escherichia coli O157:H7 via Contaminated Ground Beef","volume":"66","author":"MR Wachtel","year":"2003","journal-title":"J Food Prot"},{"issue":"1","key":"pone.0290182.ref086","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4315\/0362-028X-70.1.109","article-title":"Management of risk of microbial cross-contamination from uncooked frozen hamburgers by alcohol-based hand sanitizer","volume":"70","author":"DW Schaffner","year":"2007","journal-title":"J Food Prot"},{"issue":"15","key":"pone.0290182.ref087","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1001\/archinte.160.15.2380","article-title":"Where\u2019s the Beef? The Role of Cross-contamination in 4 Chain Restaurant\u2013Associated Outbreaks of Escherichia coli O157:H7 in the Pacific Northwest","volume":"160","author":"LA Jackson","year":"2000","journal-title":"Arch intern med"},{"unstructured":"FSAI. Microbiological safety of raw minced beef and beef burgers on retail sale in Ireland (11NS1). MicroBiology ed2013. Available from: https:\/\/www.fsai.ie\/getmedia\/691d10f1-ecb4-4ada-8a2a-82db130be9d3\/raw-minced-beef-survey-final.pdf?ext=.pdf","key":"pone.0290182.ref088"},{"issue":"1","key":"pone.0290182.ref089","first-page":"5","article-title":"Cooking inactivation of Shiga toxin producing Escherichia coli (STEC) in non-intact beefs\u2014a mini-review","volume":"2","author":"C. Shen","year":"2014","journal-title":"J Food Processing & Beverages"},{"unstructured":"FSAI. An investigation of the most appropriate z-value to be used in calculating \u2018equivalent cooks\u2019 for beef burgers in food business establishments. Dublin, Ireland. 2018. p. 36. Available from: https:\/\/www.fsai.ie\/getmedia\/52b3e2ce-a798-45d5-ab63-9fe0961769d1\/burgers_cooking_slow.pdf?ext=.pdf.","key":"pone.0290182.ref090"},{"key":"pone.0290182.ref091","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.meatsci.2013.09.009","article-title":"Consumer preferences, internal color and reduction of shigatoxigenic Escherichia coli in cooked hamburgers","volume":"96","author":"E R\u00f8ssvoll","year":"2014","journal-title":"Meat Sci"},{"issue":"4","key":"pone.0290182.ref092","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3201\/eid1104.040739","article-title":"Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982\u20132002","volume":"11","author":"JM Rangel","year":"2005","journal-title":"Emerg Infect Dis"},{"issue":"12","key":"pone.0290182.ref093","first-page":"21","article-title":"World Health Organization estimates of the global and regional disease burden of 22 foodborne bacterial, protozoal, and viral diseases, 2010: A data synthesis","volume":"12","author":"MD Kirk","journal-title":"PLoS Med. 2015"},{"unstructured":"JEMRA. Joint FAO\/WHO. Shiga toxin-producing Escherichia coli (STEC) and food: attribution, characterization, and monitoring. 2018. Microbiological risk assessment series 31. Available from: https:\/\/apps.who.int\/iris\/handle\/10665\/272871.","key":"pone.0290182.ref094"},{"unstructured":"Bolsa de Comercio de Rosario. 2021 [cited 2022 Aug 9]. Available from: https:\/\/www.bcr.com.ar\/es\/mercados\/investigacion-y-desarrollo\/informativo-semanal\/noticias-informativo-semanal\/el-2021-0.","key":"pone.0290182.ref095"},{"key":"pone.0290182.ref096","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/978-3-319-45092-6_5","article-title":"Enterohemorrhagic (Shiga Toxin-Producing) Escherichia coli","author":"M Rivas","year":"2016","journal-title":"Escherichia coli in the Americas"},{"unstructured":"\u00c1rea de Vigilancia de la Salud de la Direcci\u00f3n de Epidemiolog\u00eda. Ministerio de Salud de la Naci\u00f3n Argentina. Bolet\u00edn Integrado de Vigilancia. N\u02da 329\u2014SE 39\u20132016. Ciudad Aut\u00f3noma de Buenos Aires2016.","key":"pone.0290182.ref097"},{"issue":"III","key":"pone.0290182.ref098","article-title":"Epidemiolog\u00eda del S\u00edndrome Ur\u00e9mico Hemol\u00edtico en Argentina. Diagn\u00f3stico del agente etiol\u00f3gico, reservorios y v\u00edas de transmisi\u00f3n","volume":"66","author":"M Rivas","year":"2006","journal-title":"Medicina (B Aires)"},{"key":"pone.0290182.ref099","first-page":"127","article-title":"Epidemiolog\u00eda del s\u00edndrome ur\u00e9mico hemol\u00f3tico en dos regiones de la provincia de Buenos Aires","volume":"73","author":"M Rivero","year":"2013","journal-title":"Medicina (B. Aires)"},{"issue":"4","key":"pone.0290182.ref100","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1371\/journal.pone.0019136","article-title":"Antibody response to Shiga toxins in Argentinean children with enteropathic hemolytic uremic syndrome at acute and long-term follow-up periods","volume":"6","author":"RJ Fernandez-Brando","year":"2011","journal-title":"PLoS One"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0290182","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T06:35:25Z","timestamp":1702967725000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0290182"}},"subtitle":[],"editor":[{"given":"Chisoni","family":"Mumba","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,8,17]]},"references-count":100,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0290182","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2023,8,17]]}}}