{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:13:01Z","timestamp":1791288781077,"version":"4.3.0"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T00:00:00Z","timestamp":1693353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Agency of Research of France","award":["PLAS\u2019STER ANR-12-TECS-0007"],"award-info":[{"award-number":["PLAS\u2019STER ANR-12-TECS-0007"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The sterilization of medical devices is paramount to achieve an acceptable level of sterility assurance and to prevent hospital-acquired infections. However, some medical devices cannot be sterilized by usual processes such as autoclave (AC) and gamma-ray irradiation (GI). A new non-thermal plasma (NTP) process using sealed bag that preserves the sterile state of the devices could be used as an alternative sterilization method. The aim of the study was to assess the cytocompatibility of titanium and poly(etheretherketone) (PEEK) surfaces after O<jats:sub>2<\/jats:sub>-NTP sterilization compared to GI and AC. MG-63 osteoblast-like cells were seeded on titanium (TA6V) and PEEK disks sterilized by AC, GI and O<jats:sub>2<\/jats:sub>-NTP. The cells\u2019 viability and proliferation, determined by WST-1 and DNA quantification respectively, were enhanced whatever the material types from 3 to 10 days. When seeded on titanium, MG-63 cells showed a higher viability and proliferation after GI and O<jats:sub>2<\/jats:sub>-NTP treatment compared to AC treatment. When cultured on PEEK, MG-63 cells showed a higher viability after O<jats:sub>2<\/jats:sub>-NTP treatment. No difference of proliferation was observed whatever the sterilization processes. The cell colonization of the materials\u2019 surface was confirmed by scanning electron microscopy. Lactate dehydrogenase (LDH) assay revealed no cytotoxicity. Thus, O<jats:sub>2<\/jats:sub>-NTP led to similar cell responses to AC and GI and could be a cost-effective alternative process to the usual sterilization methods for fragile medical devices.<\/jats:p>","DOI":"10.1371\/journal.pone.0290820","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T17:26:28Z","timestamp":1693416388000},"page":"e0290820","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cytocompatibility of titanium and poly(etheretherketone) surfaces after O2 non-thermal plasma sterilization"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4341-7311","authenticated-orcid":true,"given":"Christina","family":"Maillet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0483-3662","authenticated-orcid":true,"given":"Florence M.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3671-530X","authenticated-orcid":true,"given":"Florian","family":"Le Bras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederic","family":"Velard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Guillaume","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2651-9372","authenticated-orcid":true,"given":"Sophie C.","family":"Gangloff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1569-7085","authenticated-orcid":true,"given":"Marie-Paule","family":"Gelle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,8,30]]},"reference":[{"key":"pone.0290820.ref001","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.dental.2020.10.007","article-title":"The effects of three cold plasma treatments on the osteogenic activity and antibacterial property of PEEK","volume":"37","author":"C Liu","year":"2021","journal-title":"Dent Mater"},{"issue":"4","key":"pone.0290820.ref002","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/ppap.201100034","article-title":"Cell Adhesion to PEEK Treated by Plasma Immersion Ion Implantation and Deposition for Active Medical Implants","volume":"9","author":"F Awaja","year":"2012","journal-title":"Plasma Process Polym"},{"issue":"5","key":"pone.0290820.ref003","doi-asserted-by":"crossref","first-page":"1966","DOI":"10.1016\/j.actbio.2011.11.026","article-title":"Effect of cleaning and sterilization on titanium implant surface properties and cellular response","volume":"8","author":"JH Park","year":"2012","journal-title":"Acta Biomater"},{"issue":"22","key":"pone.0290820.ref004","doi-asserted-by":"crossref","first-page":"5596","DOI":"10.3390\/ijms20225596","article-title":"Cytocompatibility of Titanium, Zirconia and Modified PEEK after Surface Treatment Using UV Light or Non-Thermal Plasma","volume":"20","author":"L Guo","year":"2019","journal-title":"Int J Mol Sci"},{"issue":"1","key":"pone.0290820.ref005","doi-asserted-by":"crossref","first-page":"e15","DOI":"10.1016\/j.dental.2020.09.020","article-title":"The impact of different low-pressure plasma types on the physical, chemical and biological surface properties of PEEK","volume":"37","author":"Q Fu","year":"2021","journal-title":"Dent Mater"},{"key":"pone.0290820.ref006","unstructured":"International Organization for Standardization. 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