{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T17:54:09Z","timestamp":1793555649338,"version":"4.5.0"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Innovation Project of Guangxi Graduate Education","award":["NO: YCBZ2021047"],"award-info":[{"award-number":["NO: YCBZ2021047"]}]},{"name":"Research Basic Ability Enhancement Project of Young and Middle-aged Teachers in Guangxi Universities","award":["NO: 2021KY0096"],"award-info":[{"award-number":["NO: 2021KY0096"]}]},{"name":"Excellent Medical Talents Training Program of the First Affiliated Hospital of Guangxi Medical University,","id":[{"id":"https:\/\/ror.org\/030sc3x20","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2020GXNSFAA297084"],"award-info":[{"award-number":["2020GXNSFAA297084"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001406","name":"Guangxi Medical High-level Backbone Talent \"139\" Plan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001406","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Key Laboratory of Children's Disease Research in Guangxi\u2019s Colleges and Universities, Education Department of Guangxi Zhuang Autonomous Region"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In the past two years, studies have found a significant increase in neutrophil extracellular traps (NETs) in patients with IgA vasculitis (IgAV), which is correlated with the severity of the disease. NETs have been reported as an intervention target in inflammatory and autoimmune diseases. This study aimed to investigate the effect of targeted degradation of NETs using DNase I in IgAV rat model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Twenty-four Sprague-Dawley rats were randomly divided into three groups: the IgAV model group, the DNase I intervention group and the normal control group, with an average of 8 rats in each group. The model group was established by using Indian ink, ovalbumin, and Freund\u2019s complete adjuvant. In the intervention group, DNase I was injected through tail vein 3 days before the end of established model. The circulating cell free-DNA (cf-DNA) and myeloperoxidase-DNA (MPO-DNA) were analyzed. The presence of NETs in the kidney, gastric antrum and descending duodenum were detected using multiple fluorescences immunohistochemistry and Western blots. Morphological changes of the tissues were observed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>After the intervention of DNase I, there was a significant reduction in cf-DNA and MPO-DNA levels in the intervention group compared to the IgAV model group (all P&lt;0.001). The presence of NETs in renal, gastric, and duodenal tissues of the intervention group exhibited a significant decrease compared to the IgAV model group (P &lt; 0.01). Moreover, the intervention group demonstrated significantly lower levels of renal MPO and citrullinated histone H3 (citH3) protein expression when compared to the IgAV model group (all P &lt; 0.05). The HE staining results of intervention group demonstrated a significant reduction in congestion within glomerular and interstitial capillaries. Moreover, there was a notable improvement in gastric and intestinal mucosa necrosis, congestion and bleeding. Additionally, there was a substantial decrease in inflammatory cells infiltration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The degradation of NETs can be targeted by DNase I to mitigate tissue damage in IgAV rat models. Targeted regulation of NETs holds potential as a therapeutic approach for IgAV.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0291592","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T13:25:55Z","timestamp":1698758755000},"page":"e0291592","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":26,"title":["DNase I targeted degradation of neutrophil extracellular traps to reduce the damage on IgAV rat"],"prefix":"10.1371","volume":"18","author":[{"given":"Xiu-Qi","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Sen","family":"Zou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Yun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5463-8048","authenticated-orcid":true,"given":"Yuan-Han","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,10,31]]},"reference":[{"issue":"1","key":"pone.0291592.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/art.37715","article-title":"2012 revised International Chapel Hill Consensus Conference Nomenclature of Vasculitides.","volume":"65","author":"JC Jennette","year":"2013","journal-title":"Arthritis Rheum"},{"key":"pone.0291592.ref002","doi-asserted-by":"crossref","first-page":"912929","DOI":"10.3389\/fimmu.2022.912929","article-title":"An Emerging Role for Neutrophil Extracellular Traps in IgA Vasculitis: A Mini-Review.","volume":"13","author":"XQ Chen","year":"2022","journal-title":"Front Immunol."},{"issue":"5","key":"pone.0291592.ref003","first-page":"798","article-title":"EULAR\/PRINTO\/PRES criteria for Henoch-Schonlein purpura, childhood polyarteritis nodosa, childhood Wegener granulomatosis and childhood Takayasu arteritis: Ankara 2008.","volume":"69","author":"S Ozen","year":"2010","journal-title":"Part II: Final classification criteria. 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