{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T17:50:53Z","timestamp":1772646653250,"version":"3.50.1"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T00:00:00Z","timestamp":1696464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Abadan University of Medical Sciences","award":["98u-265"],"award-info":[{"award-number":["98u-265"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Cystic echinococcosis (CE) is a life-threatening helminthic disease caused by the <jats:italic>Echinococcus granulosus<\/jats:italic> sensulato complex. Previous evidence indicates that the host\u2019s innate immune responses against CE can combat and regulate the growth rate and mortality of hydatid cyst in the host\u2019s internal organs. However, the survival mechanisms of CE are not yet fully elucidated in the human body. In the present study, the apoptotic effects of fertile and infertile hydatid fluid (HF) were tested on murine peritoneal cells <jats:italic>in vivo<\/jats:italic> mice model. Mice were divided into five groups including; control group, fertile HF-treated peritoneal cells, infertile HF-treated peritoneal cells, protoscolices (PSCs)-treated peritoneal cells and HF+PSCs-treated peritoneal cells group. Mice groups were intraperitoneally inoculated with PBS, HF, and\/or PSCs. Afterwards, peritoneal cells were isolated and mRNA expression of STAT3, caspase-3, p73 and Smac genes were evaluated by quantitative Real-time PCR. After 48 hours of exposure, the protein levels of Smac and STAT3 was determined by western blotting technique. After 6 hours of exposure, Caspase-3 activity was also measured by fluorometric assay. The intracellular reactive oxygen species (ROS) production was examined in all groups. The mRNA expression levels of p73, caspase-3 and also Caspase-3 activity in HF+PSCs-treated peritoneal cells were higher than in the test and control groups (<jats:italic>Pv<\/jats:italic>&lt;0.05), while the mRNA expression level of anti-apoptotic STAT3 and Smac genes in HF+PSC-treated peritoneal cells were lower than in the other groups (<jats:italic>Pv<\/jats:italic>&lt;0.05). As well, the level of intracellular ROS in the fertile HCF-treated peritoneal cells, infertile HCF-treated peritoneal cells, PSC-treated peritoneal cells and HF+PSC-treated peritoneal cells groups were significantly higher than in the control group (<jats:italic>Pv<\/jats:italic>&lt;0.05).Current findings indicates that oxidative stress and p73 can trigger the apoptosis of murine peritoneal cells through modulator of HF-treated PSCs that is likely one of the hydatid cyst survival mechanisms <jats:italic>in vivo<\/jats:italic> mice model.<\/jats:p>","DOI":"10.1371\/journal.pone.0292434","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T17:38:54Z","timestamp":1696527534000},"page":"e0292434","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Tumor suppressor p73 induces apoptosis of murine peritoneal cell after exposure to hydatid cyst antigens; a possibly survival mechanism of cystic echinococcosis in vivo mice model"],"prefix":"10.1371","volume":"18","author":[{"given":"Ehsan","family":"Ahmadpour","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3009-9537","authenticated-orcid":true,"given":"Adel","family":"Spotin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ata","family":"Moghimi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Firooz","family":"Shahrivar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhad","family":"Jadidi-Niaragh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farnaz","family":"Hajizadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirous","family":"Mehrani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4032-8452","authenticated-orcid":true,"given":"Komeil","family":"Mazhab-Jafari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,10,5]]},"reference":[{"key":"pone.0292434.ref001","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.rvsc.2020.11.010","article-title":"Echinococcus granulosus sensu lato and Echinococcus multilocularis: A review","volume":"135","author":"ID Woolsey","year":"2021","journal-title":"Res Vet Sci"},{"issue":"1","key":"pone.0292434.ref002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-021-04934-9","article-title":"Epidemiology and spatial distribution of Echinococcus granulosus in sheep and goats slaughtered in a hyperendemic European Mediterranean area","volume":"14","author":"A Bosco","year":"2021","journal-title":"Parasites & Vectors"},{"issue":"13","key":"pone.0292434.ref003","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.3748\/wjg.v18.i13.1448","article-title":"Hepatic echinococcosis: clinical and therapeutic aspects","volume":"18","author":"G Nunnari","year":"2012","journal-title":"World J Gastroenterol"},{"issue":"2","key":"pone.0292434.ref004","doi-asserted-by":"crossref","first-page":"49","DOI":"10.4322\/acr.2017.012","article-title":"Primary pelvic hydatid cyst in a postmenopausal female: a surgical challenge","volume":"7","author":"S Paul","year":"2017","journal-title":"Autops Case Rep"},{"issue":"1","key":"pone.0292434.ref005","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1128\/CMR.17.1.107-135.2004","article-title":"Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern","volume":"17","author":"J Eckert","year":"2004","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"pone.0292434.ref006","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1128\/CMR.17.1.107-135.2004","article-title":"Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern","volume":"17","author":"J Eckert","year":"2004","journal-title":"Clinical microbiology reviews"},{"key":"pone.0292434.ref007","first-page":"211","volume-title":"Markell & Voge\u2019s Medical Parasitology-10th Sea Ed","author":"DT PW John","year":"2020","edition":"2"},{"issue":"3","key":"pone.0292434.ref008","doi-asserted-by":"crossref","first-page":"447","DOI":"10.4103\/2141-9248.133476","article-title":"Uncommon locations and presentations of hydatid cyst","volume":"4","author":"S Sachar","year":"2014","journal-title":"Ann Med Health Sci Res"},{"key":"pone.0292434.ref009","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1007\/s00423-003-0418-y","article-title":"Echinococcus granulosus infection: clinical presentation, medical treatment and outcome","volume":"388","author":"P. 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