{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T18:43:34Z","timestamp":1772217814165,"version":"3.50.1"},"reference-count":17,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T00:00:00Z","timestamp":1697587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Healthcare of the Republic of Kazakhstan","award":["Program No.BR11065386"],"award-info":[{"award-number":["Program No.BR11065386"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>COVID-19 vaccines have played a critical role in controlling the COVID-19 pandemic. Although overall considered safe, COVID-19 vaccination has been associated with rare but severe thrombotic events, occurring mainly in the context of adenoviral vectored vaccines. A better understanding of mechanisms underlying vaccine-induced hypercoagulability and prothrombotic state is needed to improve vaccine safety profile. We assessed changes to the biomarkers of endothelial function (endothelin, ET-1), coagulation (thrombomodulin, THBD and plasminogen activator inhibitor, PAI) and platelet activation (platelet activating factor, PAF, and platelet factor 4 IgG antibody, PF4 IgG) within a three-week period after the first (prime) and second (boost) doses of Gam-Covid-Vac, an AdV5\/AdV26-vectored COVID-19 vaccine. Blood plasma collected from vaccinees (n = 58) was assayed using ELISA assays. Participants were stratified by prior COVID-19 exposure based on their baseline SARS-CoV-2-specific serology results. We observed a significant post-prime increase in circulating ET-1, with levels sustained after the boost dose compared to baseline. ET-1 elevation following dose 2 was most pronounced in vaccinees without prior COVID-19 exposure. Prior COVID-19 was also associated with a mild increase in post-dose 1 PAI. Vaccination was associated with elevated ET-1 up to day 21 after the second vaccine dose, while no marked alterations to other biomarkers, including PF4 IgG, were seen. A role of persistent endothelial activation following COVID-19 vaccination warrants further investigation.<\/jats:p>","DOI":"10.1371\/journal.pone.0293074","type":"journal-article","created":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T13:37:10Z","timestamp":1697636230000},"page":"e0293074","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["The impact of Gam-COVID-Vac, an Adv5\/Adv26 COVID-19 vaccine, on the biomarkers of endothelial function, coagulation and platelet activation"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4668-3295","authenticated-orcid":true,"given":"Anar","family":"Turmukhambetova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7136-7921","authenticated-orcid":true,"given":"Sergey","family":"Yegorov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9065-2448","authenticated-orcid":true,"given":"Ilya","family":"Korshukov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1054-6504","authenticated-orcid":true,"given":"Valentina","family":"Barkhanskaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3515-8900","authenticated-orcid":true,"given":"Svetlana","family":"Kolesnichenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2012-2227","authenticated-orcid":true,"given":"Dmitriy","family":"Klyuyev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3227-1692","authenticated-orcid":true,"given":"Zhibek","family":"Zhumadilova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aruzhan","family":"Pralieva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laylim","family":"Absaghit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6149-3265","authenticated-orcid":true,"given":"Ruslan","family":"Belyaev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitriy","family":"Babenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gonzalo H.","family":"Hortelano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3426-5069","authenticated-orcid":true,"given":"Matthew S.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitriy","family":"Vazenmiller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7173-3138","authenticated-orcid":true,"given":"Irina","family":"Kadyrova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,10,18]]},"reference":[{"key":"pone.0293074.ref001","doi-asserted-by":"crossref","first-page":"967926","DOI":"10.3389\/fcvm.2022.967926","article-title":"Thrombotic events with or without thrombocytopenia in recipients of adenovirus-based COVID-19 vaccines","volume":"9","author":"L Cari","year":"2022","journal-title":"Front Cardiovasc Med"},{"key":"pone.0293074.ref002","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1038\/s41569-021-00665-7","article-title":"Current and novel biomarkers of thrombotic risk in COVID-19: a Consensus Statement from the International COVID-19 Thrombosis Biomarkers Colloquium","volume":"19","author":"DA Gorog","year":"2022","journal-title":"Nat Rev Cardiol"},{"key":"pone.0293074.ref003","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1056\/NEJMc2210813","article-title":"Vaccine-induced immune thrombocytopenia and thrombosis after the Sputnik V vaccine","volume":"387","author":"R Herrera-Comoglio","year":"2022","journal-title":"N Engl J Med"},{"key":"pone.0293074.ref004","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.thromres.2023.01.015","article-title":"Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. 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