{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:52:14Z","timestamp":1790830334097,"version":"4.1.0"},"reference-count":68,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2021\/11487-4"],"award-info":[{"award-number":["2021\/11487-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/25125-7"],"award-info":[{"award-number":["2019\/25125-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018694","name":"HORIZON EUROPE Marie Sklodowska-Curie Actions","doi-asserted-by":"publisher","award":["101086257"],"award-info":[{"award-number":["101086257"]}],"id":[{"id":"10.13039\/100018694","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The development of insecticide resistance in mosquitoes of public health importance has encouraged extensive research into innovative vector control methods. Terpenes are the largest among Plants Secondary Metabolites and have been increasingly studied for their potential as insecticidal control agents. Although promising, terpenes are insoluble in water, and they show low residual life which limits their application for vector control. In this study, we developed and evaluated the performances of terpenoid-based nanoemulsions (TNEs) containing myrcene and p-cymene against the dengue vector <jats:italic>Aedes aegypti<\/jats:italic> and investigated their potential toxicity against non-target organisms. Our results showed that myrcene and p-cymene showed moderate larvicidal activity against mosquito larvae compared to temephos an organophosphate widely used for mosquito control. However, we showed similar efficacy of TNEs against both susceptible and highly insecticide-resistant mosquitoes from French Guyana, hence suggesting an absence of cross-resistance with conventional insecticides. We also showed that TNEs remained effective for up to 45 days in laboratory conditions. The exposure of zebrafish to TNEs triggered behavioral changes in the fish at high doses but they did not alter the normal functioning of zebrafish organs, suggesting a good tolerability of non-target organisms to these molecules. Overall, this study provides new insights into the insecticidal properties and toxicity of terpenes and terpenoid-based formulations and confirms that TNE may offer interesting prospects for mosquito control as part of integrated vector management.<\/jats:p>","DOI":"10.1371\/journal.pone.0293124","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T18:37:08Z","timestamp":1707331028000},"page":"e0293124","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Larvicidal properties of terpenoid-based nanoemulsions against the dengue vector Aedes aegypti L. and their potential toxicity against non-target organism"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7276-3686","authenticated-orcid":true,"given":"Jonatas Lobato","family":"Duarte","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Duchon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Delello","family":"Di Filippo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6698-0545","authenticated-orcid":true,"given":"Marlus","family":"Chorilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0995-3257","authenticated-orcid":true,"given":"Vincent","family":"Corbel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,2,7]]},"reference":[{"key":"pone.0293124.ref001","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/S0140-6736(18)32560-1","article-title":"Dengue","volume":"393","author":"A Wilder-Smith","year":"2019","journal-title":"The Lancet"},{"key":"pone.0293124.ref002","first-page":"504","article-title":"The global distribution and burden of dengue","volume":"496","author":"S Bhatt","year":"2013","journal-title":"Nature 2013 496:7446"},{"key":"pone.0293124.ref003","doi-asserted-by":"crossref","first-page":"12986","DOI":"10.1038\/ncomms12986","article-title":"Massive yet grossly underestimated global costs of invasive insects","volume":"7","author":"CJA Bradshaw","year":"2016","journal-title":"Nat Commun"},{"key":"pone.0293124.ref004","article-title":"A gest\u00e3o de doen\u00e7as infecto-contagiosas: o impacto do dengue na sa\u00fade p\u00fablica","author":"IC Romero","year":"2014","journal-title":"Trabalho de Conclus\u00e3o de curso, Universidade Estadual Paulista J\u00falio de Mesquita FIlho"},{"key":"pone.0293124.ref005","first-page":"571","article-title":"High and rising economic costs of biological invasions worldwide","volume":"592","author":"C Diagne","year":"2021","journal-title":"Nature 2021 592:7855"},{"key":"pone.0293124.ref006","doi-asserted-by":"crossref","first-page":"S17","DOI":"10.2149\/tmh.2011-S04","article-title":"Dengue Vectors and their Spatial Distribution","volume":"39","author":"Y. 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Pavela","year":"2015","journal-title":"Ind Crops Prod"},{"key":"pone.0293124.ref031","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.indcrop.2013.03.016","article-title":"Larvicidal activities of wood and leaf essential oils and ethanolic extracts from Cunninghamia konishii Hayata against the dengue mosquitoes","volume":"47","author":"SS Cheng","year":"2013","journal-title":"Ind Crops Prod"},{"key":"pone.0293124.ref032","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1002\/ps.1693","article-title":"Insecticidal activities of leaf and twig essential oils from Clausena excavata against Aedes aegypti and Aedes albopictus larvae","volume":"65","author":"S-S Cheng","year":"2009","journal-title":"Pest Manag Sci"},{"key":"pone.0293124.ref033","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.biortech.2007.11.060","article-title":"Variations in insecticidal activity and chemical compositions of leaf essential oils from Cryptomeria japonica at 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Indies).","volume":"10","author":"S Marcombe","year":"2009","journal-title":"BMC Genomics"},{"key":"pone.0293124.ref040","doi-asserted-by":"crossref","first-page":"e0243992","DOI":"10.1371\/journal.pone.0243992","article-title":"CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies.","volume":"16","author":"Y Epelboin","year":"2021","journal-title":"PLoS One"},{"key":"pone.0293124.ref041","first-page":"1","article-title":"Guidelines for laboratory and field testing of mosquito larvicides.","author":"WORLD HEALTH ORGANIZATION","year":"2005","journal-title":"World Health Organization."},{"key":"pone.0293124.ref042","author":"World Health Organization","year":"2016","journal-title":"Monitoring and managing insecticide resistance in Aedes mosquito populations Interim guidance for entomologists"},{"key":"pone.0293124.ref043","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/0165-6147(83)90490-X","article-title":"Analysis of combined drug effects: a new look at a very old problem","volume":"4","author":"T-C Chou","year":"1983","journal-title":"Trends Pharmacol Sci"},{"key":"pone.0293124.ref044","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0304-3959(02)00041-6","article-title":"The interaction index: a measure of drug synergism","volume":"98","author":"RJ Tallarida","year":"2002","journal-title":"Pain"},{"key":"pone.0293124.ref045","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1093\/jmedent\/43.6.1190","article-title":"Laboratory evaluation of pyriproxyfen and spinosad, alone and in combination, against Aedes aegypti larvae","volume":"43","author":"F Darriet","year":"2006","journal-title":"J Med Entomol"},{"key":"pone.0293124.ref046","first-page":"18","article-title":"Obtainment and Study of the Toxicity of Perillyl Alcohol Nanoemulsion on Zebrafish (Danio rerio)","volume":"4","author":"Duarte JLD Sousa GC de","year":"2016","journal-title":"J Nanomed Res"},{"key":"pone.0293124.ref047","doi-asserted-by":"crossref","DOI":"10.1787\/9789264071049-en","volume-title":"Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure.","author":"OECD","year":"2022"},{"key":"pone.0293124.ref048","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1007\/s10787-017-0438-9","article-title":"Anti-inflammatory activity of nanoemulsions of essential oil from Rosmarinus officinalis L.: in vitro and in zebrafish studies","volume":"26","author":"RS Borges","year":"2018","journal-title":"Inflammopharmacology"},{"key":"pone.0293124.ref049","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1177\/0960327119878257","article-title":"Reproductive toxicity of the hydroethanolic extract of the flowers of Acmella oleracea and spilanthol in zebrafish: In vivo and in silico evaluation","volume":"39","author":"GC de 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aegypti, the common vector of Dengue virus","volume":"34","author":"AA Almadiy","year":"2022","journal-title":"Journal of Essential Oil Research"},{"key":"pone.0293124.ref059","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-019-3870-4","article-title":"Yeast-encapsulated essential oils: A new perspective as an environmentally friendly larvicide.","volume":"13","author":"MJ Workman","year":"2020","journal-title":"Parasit Vectors"},{"key":"pone.0293124.ref060","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1186\/s13071-021-04733-2","article-title":"High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil","volume":"14","author":"B Gomes","year":"2021","journal-title":"Parasit Vectors"},{"key":"pone.0293124.ref061","first-page":"1608","article-title":"Synthesis and Technology of Nanoemulsion-Based Pesticide Formulation","volume":"10","author":"IF Mustafa","year":"2020","journal-title":"terials 2020, Vol 10, Page 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