{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T10:25:00Z","timestamp":1779272700023,"version":"3.51.4"},"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agence Nationale pour la Recherche","award":["ANR19-CE35-0013-01 SurVi"],"award-info":[{"award-number":["ANR19-CE35-0013-01 SurVi"]}]},{"name":"Medical Research Fundation","award":["EQU202003010328"],"award-info":[{"award-number":["EQU202003010328"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Vibrio cholerae<\/jats:italic>, the causative agent of cholera epidemics, is a rod-shaped bacterium with a highly polarized cellular organization. It can survive harmful growth conditions by entering a non-proliferating spheroplast state, which involves loss of the cell envelope and polarity. How polarized rod organization cells are formed when the spheroplasts exit the non-proliferating state remains largely uncharacterized. To address this question, we investigated how L-arabinose-induced <jats:italic>V<\/jats:italic>. <jats:italic>cholerae<\/jats:italic> spheroplasts return to growth. We found that <jats:italic>de novo<\/jats:italic> morphogenesis started with the elimination of an excess of periplasm, which was immediately followed by cell elongation and the formation of cell branches with a diameter similar to that of normal <jats:italic>V<\/jats:italic>. <jats:italic>cholerae<\/jats:italic> cells. Periplasm elimination was driven by bifunctional peptidoglycan synthases involved in cell-wall maintenance, the aPBPs. Elongation and branching relied on the MreB-associated monofunctional peptidoglycan synthase PBP2. The cell division monofunctional peptidoglycan synthase FtsI was not involved in any of these processes. However, the FtsK cell division protein specifically targeted the sites of vesicle extrusion. Genetic material was amplified by synchronous waves of DNA replication as periplasmic elimination began. The HubP polarity factor targeted the tip of the branches as they began to form. However, HubP-mediated polarization was not involved in the efficiency of the recovery process. Finally, our results suggest that the positioning of HubP and the activities of the replication terminus organizer of the two <jats:italic>V<\/jats:italic>. <jats:italic>cholerae<\/jats:italic> chromosomes, MatP, are independent of cell division. Taken together, these results confirm the interest of L-arabinose-induced <jats:italic>V<\/jats:italic>. <jats:italic>cholerae<\/jats:italic> spheroplasts to study how cell shape is generated and shed light on the <jats:italic>de novo<\/jats:italic> establishment of the intracellular organization and cell polarization in <jats:italic>V<\/jats:italic>. <jats:italic>cholerae<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pone.0293276","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T17:42:12Z","timestamp":1698342132000},"page":"e0293276","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Recovery of Vibrio cholerae polarized cellular organization after exit from a non-proliferating spheroplast state"],"prefix":"10.1371","volume":"18","author":[{"given":"Anthony","family":"Goudin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Luc","family":"Ferat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Possoz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9170-9896","authenticated-orcid":true,"given":"Fran\u00e7ois-Xavier","family":"Barre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9276-7262","authenticated-orcid":true,"given":"Elisa","family":"Galli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,10,26]]},"reference":[{"key":"pone.0293276.ref001","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1128\/MMBR.00001-06","article-title":"The Selective Value of Bacterial Shape","volume":"70","author":"KD Young","year":"2006","journal-title":"Microbiology and Molecular Biology Reviews"},{"key":"pone.0293276.ref002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/mr.57.1.1-33.1993","article-title":"Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis","volume":"57","author":"J. 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