{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:42:15Z","timestamp":1787060535784,"version":"build-2736575974"},"reference-count":30,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T00:00:00Z","timestamp":1699228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Medical Parasitology, PGIMER, Chandigarh"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Leishmaniasis is a vector-borne parasitic infection caused by the infective bite of female Phlebotomine sandflies. Treatment of leishmaniasis by conventional synthetic compounds is met by challenges pertaining to adverse effects which call for the discovery of newer anti-leishmanial molecules. This study was performed to evaluate the effect and modes of action of a sesquiterpene alcoholic molecule Farnesol on Leishmania major, the causative agent of Zoonotic CL. The cytotoxic effect of Farnesol against\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>major<\/jats:italic>\n                    promastigotes, amastigotes and macrophages was assessed by MTT test and counting. The IC\n                    <jats:sub>50<\/jats:sub>\n                    on promastigotes by Farnesol on\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>major<\/jats:italic>\n                    was also evaluated by flow cytometry. In the findings, promastigotes were reduced at 167\u03bcM. The mean numbers of\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>major<\/jats:italic>\n                    amastigotes in macrophages were significantly decreased on exposure to Farnesol at 172\u03bcM. In addition, Farnesol induced significant apoptosis dose-dependent on\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>major<\/jats:italic>\n                    promastigotes.\n                    <jats:italic>In silico<\/jats:italic>\n                    protein-ligand_binding analyses indicated the effect of Farnesol in perturbation of the ergosterol synthesis pathway of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    with attributes suggesting inhibition of Lanosterol-\u03b1-demethylase, the terminal enzyme of ergosterol synthesis machinery. Findings from flow cytometry reveal the role of Farnesol in apoptosis-induced killing in promastigotes. Farnesol was effective at very lower concentrations when compared to Paromomycin. Further studies are crucial to evaluate the therapeutic potential of Farnesol alone or in combination with other conventional drugs in animal models.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0293290","type":"journal-article","created":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T13:30:17Z","timestamp":1699277417000},"page":"e0293290","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Antiparasitic effect of Farnesol against Leishmania major: A rationale from in vitro and in silico investigations"],"prefix":"10.1371","volume":"18","author":[{"given":"Harshita","family":"Sharma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9833-3265","authenticated-orcid":true,"given":"Rakesh","family":"Sehgal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nishant","family":"Shekhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaurav","family":"Shoeran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Upninder","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bikash","family":"Medhi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,11,6]]},"reference":[{"issue":"1","key":"pone.0293290.ref001","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1039\/C5CS00674K","article-title":"New approaches from nanomedicine for treating leishmaniasis","volume":"45","author":"V. 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