{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:07:53Z","timestamp":1787958473832,"version":"build-2784847793"},"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Candida auris<\/jats:italic> is a multidrug-resistant pathogen, that is a well-known cause of nosocomial infections. This pathogen is being identified using advanced diagnostic approaches and epidemiological typing procedures. In underdeveloped nations, several researchers developed and validated a low-cost approach for reliably identifying <jats:italic>Candida auris<\/jats:italic>. The goal of this study was to assess the burden of <jats:italic>Candida auris<\/jats:italic> in different teaching hospitals of Lahore and to limit its spread to minimize hospital-related illnesses. <jats:italic>Candida<\/jats:italic> isolates were obtained from various tertiary care institutions in Lahore in the form of culture on various culture plates. Sabouraud agar culture plates were used to culture the <jats:italic>Candida<\/jats:italic> spp. Fluconazole-resistant <jats:italic>Candida<\/jats:italic> species were chosen for further identification using VITEK 2 Compact ID and molecular identification using species-specific PCR assay. The current study obtained 636 <jats:italic>Candida<\/jats:italic> samples from several tertiary care institutions in Lahore. Fluconazole resistance was found in 248 (38.9%) of 636 <jats:italic>Candida<\/jats:italic> samples. No isolate was identified as <jats:italic>Candida auris<\/jats:italic> by VITEK 2 Compact ID and real-time PCR-based molecular identification. Thus with limited resources, these two methods may serve as useful screens for <jats:italic>Candida auris<\/jats:italic>. However, it should be screened all over the country to limit its spread to break the chain of nosocomial infections.<\/jats:p>","DOI":"10.1371\/journal.pone.0293390","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T17:27:43Z","timestamp":1698168463000},"page":"e0293390","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Screening of C. auris among Candida isolates from various tertiary care institutions in Lahore by VITEK 2 and real time PCR based molecular technique"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5291-9228","authenticated-orcid":true,"given":"Zill-e-","family":"Huma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sidrah","family":"Saleem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Imran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kokab","family":"Jabeen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faiqa","family":"Arshad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Amar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,10,24]]},"reference":[{"issue":"1","key":"pone.0293390.ref001","doi-asserted-by":"crossref","first-page":"e00029","DOI":"10.1128\/CMR.00029-17","article-title":"Candida 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