{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T17:36:37Z","timestamp":1793381797428,"version":"4.5.0"},"reference-count":25,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T00:00:00Z","timestamp":1699228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007859","name":"Graduate School, Chiang Mai University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007859","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Regions with a high prevalence of \u03b1-thalassemia (\u03b1-thal) require simple, rapid, and accurate tests for carrier screening and prenatal diagnosis. Diagnosis of multiple deletions in a single tube is necessary to clearly identify individuals with \u03b1\n                    <jats:sup>0<\/jats:sup>\n                    -thalassemia in the routine setting, especially in at-risk couples. Therefore, we aimed to develop a single-tube multiplex real-time PCR with EvaGreen and high-resolution melting (HRM) analysis for the identification of \u03b1\n                    <jats:sup>0<\/jats:sup>\n                    -thalassemia Southeast Asian (SEA), Thai and Chiang Rai (CR) type deletions. The results of the HRM analysis indicated that the amplified fragments from \u03b1\n                    <jats:sup>0<\/jats:sup>\n                    -thal--\n                    <jats:sup>CR<\/jats:sup>\n                    ,--\n                    <jats:sup>THAI<\/jats:sup>\n                    ,--\n                    <jats:sup>SEA<\/jats:sup>\n                    , and the wild-type \u03b1-globin gene had specific peak heights at mean melting temperature (T\n                    <jats:sub>m<\/jats:sub>\n                    ) values of 85.40\u00b0C, 86.50\u00b0C, 87.65\u00b0C, and 91.04\u00b0C, respectively. The frequencies of \u03b1\n                    <jats:sup>0<\/jats:sup>\n                    -thal--\n                    <jats:sup>SEA<\/jats:sup>\n                    ,--\n                    <jats:sup>THAI<\/jats:sup>\n                    ,--\n                    <jats:sup>CR<\/jats:sup>\n                    obtained from routine testing in 2,135 samples were 17.89%, 0.19% and 0.19%, respectively. This method would be useful for preventing Hb Bart\u2019s hydrops fetalis. Detection of multiple deletions in a single run is cost-effective, highly accurate and timesaving. This technique could enable wider \u03b1-thalassemia diagnosis in high prevalence areas and served as an example for thalassemia routine setting.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0293838","type":"journal-article","created":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T13:25:54Z","timestamp":1699277154000},"page":"e0293838","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Single-tube multiplex real-time PCR with EvaGreen and high-resolution melting analysis for diagnosis of \u03b10-thalassemia--SEA,--THAI, and--CR type deletions"],"prefix":"10.1371","volume":"18","author":[{"given":"Chedtapak","family":"Ruengdit","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoo","family":"Punyamung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nutjeera","family":"Intasai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0280-5569","authenticated-orcid":true,"given":"Sakorn","family":"Pornprasert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,11,6]]},"reference":[{"issue":"5","key":"pone.0293838.ref001","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1182\/blood.V73.5.1081.1081","article-title":"A review of the molecular genetics of the human alpha-globin gene cluster","volume":"73","author":"DR 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