{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T18:48:42Z","timestamp":1769194122740,"version":"3.49.0"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T00:00:00Z","timestamp":1699488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Horserace Betting and Levy Board","award":["Prj793"],"award-info":[{"award-number":["Prj793"]}]},{"name":"Horserace Betting and Levy Board","award":["Prj793"],"award-info":[{"award-number":["Prj793"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Equine asthma, previously known as Recurrent Airway Obstruction (RAO) or Inflammatory Airway Disease (IAD), is an often-debilitating condition that may severely affect both performance and quality of life. Research is hindered by the low sample numbers of subjects recruited to studies, a consequence in part of the invasive nature of the sampling methods of bronchial brushing and biopsy. We present an alternative method of sampling equine airway epithelial cells, the \u2018nasal brush method\u2019 (NBM). Obtained by light brushing of the ventral meatus whilst the horse is under standing sedation, these cells express the same markers of differentiation as their deeper counterparts. Grown as 3-D spheroids or as air-liquid interface cultures, nasal epithelial cells are responsive to the inflammatory cytokine interleukin-13. This may be attenuated by modulation of the Notch signalling pathway using the gamma-secretase inhibitor Semagecestat; a previously unreported finding that cements the link between equine and human asthma research and strengthens the case for a One Health approach in researching asthma pathophysiology and therapeutic intervention.<\/jats:p>","DOI":"10.1371\/journal.pone.0293956","type":"journal-article","created":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T18:24:28Z","timestamp":1699554268000},"page":"e0293956","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Investigation of nasal epithelial cells as a surrogate for bronchial epithelial cells in the research of equine asthma"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1521-3725","authenticated-orcid":true,"given":"Diane Frances","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David James","family":"Everest","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Cooley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8073-8069","authenticated-orcid":true,"given":"Mark Andrew","family":"Chambers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,11,9]]},"reference":[{"issue":"5","key":"pone.0293956.ref001","doi-asserted-by":"crossref","first-page":"e19678","DOI":"10.1371\/journal.pone.0019678","article-title":"Muc5b is the major polymeric mucin in mucus from thoroughbred horses with and without airway mucus accumulation","volume":"6","author":"K Rousseau","year":"2011","journal-title":"PLoS One"},{"issue":"2","key":"pone.0293956.ref002","doi-asserted-by":"crossref","first-page":"113","DOI":"10.2746\/042516406776563305","article-title":"Factors associated with failure of thoroughbred horses to train and race","volume":"38","author":"S Wilsher","year":"2006","journal-title":"Equine Vet J"},{"issue":"4","key":"pone.0293956.ref003","doi-asserted-by":"crossref","first-page":"300","DOI":"10.2746\/042516406777749191","article-title":"Effect of tracheal mucus and tracheal cytology on racing performance in Thoroughbred racehorses","volume":"38","author":"SJ Holcombe","year":"2006","journal-title":"Equine Vet J"},{"issue":"3","key":"pone.0293956.ref004","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.tvjl.2008.08.015","article-title":"Association of increased tracheal mucus accumulation with poor willingness to perform in show-jumpers and dressage horses","volume":"182","author":"A Widmer","year":"2009","journal-title":"Vet J"},{"key":"pone.0293956.ref005","unstructured":"Murray I. 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