{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:32:53Z","timestamp":1791289973409,"version":"4.3.0"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The emergence of antimicrobial resistance in commensal bacteria poses a serious public health burden worldwide. Commensals can disseminate the resistance genes to pathogenic bacteria causing life-threatening infections. This cross-sectional study was designed to investigate the antimicrobial resistance pattern and molecular mechanism(s) of ciprofloxacin resistance in commensal <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> from three major one health components (humans, animals and the environment) in Bangladesh. Samples were randomly collected from broiler chickens, broiler farm environments and hospitalized human patients from the same geographical area. Isolation and identification of <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> were performed following standard bacteriological techniques. Antimicrobial susceptibility testing (AST) was performed by disk diffusion and broth microdilution methods. Mutation at the quinolone-resistance determining region (QRDR) was analyzed by sequencing. Of 450 samples, a total of 287 (63.8%; 95% CI 59.2\u201368.1%) <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> strains was isolated, where 240 (83.6%; 95% CI 78.9\u201387.5%) strains were phenotypically resistant to ciprofloxacin. The prevalence of ciprofloxacin-resistant <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> in broiler chicken, broiler farm environments and hospitalized human patients are 77.6%, 88.8% and 89% respectively. In AST against nine antimicrobials, all the isolates were found to be multidrug-resistant (MDR). The minimum inhibitory concentration (MIC) of ciprofloxacin was ranged from 4 to &gt;128mg\/L. Point mutations were detected in several sites of QRDR, specifically at 83 and 87 amino acid positions in <jats:italic>gyrA<\/jats:italic> gene, and 56, 57, 78, 80 and 84 amino acid positions in <jats:italic>parC<\/jats:italic> gene. Mutations resulted in amino acid substitutions. Phylogenetic analysis of <jats:italic>gyrA<\/jats:italic> and <jats:italic>parC<\/jats:italic> gene sequences showed a close relationship between the strains isolated from different sources. This study demonstrates a high prevalence of ciprofloxacin resistance in commensal <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> in humans, animals and environment interface and their genealogically similarity poses an alarming public health consequence.<\/jats:p>","DOI":"10.1371\/journal.pone.0294043","type":"journal-article","created":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T18:31:46Z","timestamp":1700505106000},"page":"e0294043","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["High prevalence of ciprofloxacin resistance in Escherichia coli isolated from chickens, humans and the environment: An emerging one health 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