{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:38:03Z","timestamp":1777336683587,"version":"3.51.4"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:00:00Z","timestamp":1700006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005999","name":"Bangladesh Council of Scientific and Industrial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005999","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The raising concern of drug resistance, having substantial impacts on public health, has instigated the search of new natural compounds with substantial medicinal activity. In order to find out a natural solution, the current study has utilized prodigiosin, a linear tripyrrole red pigment, as an active ingredient to control bacterial proliferation and prevent cellular oxidation caused by ROS (Reactive Oxygen Species). A prodigiosin-producing bacterium BRL41 was isolated from the ancient Barhind soil of BCSIR Rajshahi Laboratories, Bangladesh, and its morphological and biochemical characteristics were investigated. Whole genome sequencing data of the isolate revealed its identity as <jats:italic>Serratia<\/jats:italic> sp. and conferred the presence of prodigiosin gene cluster in the bacterial genome. \u201cProdigiosin NRPS\u201d, among the 10 analyzed gene clusters, showed 100% similarity with query sequences where pigC, pigH, pigI, and pigJ were identified as fundamental genes for prodigiosin biosynthesis. Some other prominent clusters for synthesis of ririwpeptides, yersinopine, trichrysobactin were also found in the chromosome of BRL41, whilst the rest displayed less similarity with query sequences. Except some first-generation beta-lactam resistance genes, no virulence and resistance genes were found in the genome of BRL41. Structural illumination of the extracted red pigment by spectrophotometric scanning, Thin-Layer Chromatography (TLC), Fourier Transform Infrared Spectroscopy (FTIR), and change of color at different pH solutions verified the identity of the isolated compound as prodigiosin. <jats:italic>Serratia<\/jats:italic> sp. BRL41 attained its maximum productivity 564.74 units\/cell at temperature 30\u02daC and pH 7.5 in two-fold diluted nutrient broth medium. The compound exhibited promising antibacterial activity against Gram-positive and Gram-negative bacteria with MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values ranged from 3.9 to15.62 \u03bcg\/mL and 7.81 to 31.25 \u03bcg\/mL respectively. At concentration 500 \u03bcg\/mL, except in <jats:italic>Salmonella enterica<\/jats:italic> ATCC-10708, prodigiosin significantly diminished biofilm formed by <jats:italic>Listeria monocytogens<\/jats:italic> ATCC-3193, <jats:italic>Pseudomonas aeruginosa<\/jats:italic> ATCC-9027, <jats:italic>Escherichia coli (<\/jats:italic>environmental isolate<jats:italic>)<\/jats:italic>, <jats:italic>Staphylococcus aureus (<\/jats:italic>environmental isolate). Cellular glutathione level (GSH) was elevated upon application of 250 and 500 \u03bcg\/mL pigment where 125 \u03bcg\/mL failed to show any free radical scavenging activity. Additionally, release of cellular components in growth media of both Gram-positive and Gram-negative bacteria were facilitated by the extract that might be associated with cell membrane destabilization. Therefore, the overall findings of antimicrobial, antibiofilm and antioxidant activities suggest that in time to come prodigiosin might be a potential natural source to treat various diseases and infections.<\/jats:p>","DOI":"10.1371\/journal.pone.0294054","type":"journal-article","created":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T18:39:52Z","timestamp":1700073592000},"page":"e0294054","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["In silico exploration of Serratia sp. BRL41 genome for detecting prodigiosin Biosynthetic Gene Cluster (BGC) and in vitro antimicrobial activity assessment of secreted prodigiosin"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7581-7387","authenticated-orcid":true,"given":"Farhana","family":"Boby","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Nurul Huda","family":"Bhuiyan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barun Kanti","family":"Saha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subarna Sandhani","family":"Dey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6969-6788","authenticated-orcid":true,"given":"Anik Kumar","family":"Saha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md Jahidul","family":"Islam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahci Al","family":"Bashera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shyama Prosad","family":"Moulick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhana","family":"Jahan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Asad Uz","family":"Zaman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8722-7595","authenticated-orcid":true,"given":"Sanjana Fatema","family":"Chowdhury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Showti Raheel","family":"Naser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Salim","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Murshed Hasan","family":"Sarkar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,11,15]]},"reference":[{"key":"pone.0294054.ref001","first-page":"96","article-title":"Evaluation of Prodigiosin pigment for antimicrobial and insecticidal activities on selected bacterial pathogens & household pests","author":"B Sree","year":"2019"},{"issue":"1","key":"pone.0294054.ref002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/cid\/ciu218","article-title":"Outbreak of Serratia marcescens bloodstream infections in patients receiving parenteral nutrition prepared by a compounding pharmacy","volume":"59","author":"N Gupta","year":"2014","journal-title":"Clin Infect Dis Off Publ Infect Dis Soc Am"},{"issue":"4","key":"pone.0294054.ref003","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1128\/CMR.00017-11","article-title":"Serratia infections: from military experiments to current practice","volume":"24","author":"SD Mahlen","year":"2011","journal-title":"Clin Microbiol Rev"},{"key":"pone.0294054.ref004","article-title":"Improved Prodigiosin Production by Relieving CpxR Temperature-Sensitive Inhibition","volume":"8","author":"Y Sun","year":"2020","journal-title":"Front Bioeng Biotechnol"},{"issue":"7","key":"pone.0294054.ref005","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1007\/s13197-018-3188-9","article-title":"Antioxidant and antimicrobial activity of bioactive prodigiosin produces from Serratia marcescens using agricultural waste as a substrate","volume":"55","author":"KV Arivizhivendhan","year":"2018","journal-title":"J Food Sci Technol"},{"issue":"4","key":"pone.0294054.ref006","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1111\/j.1365-2958.2005.04602.x","article-title":"Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces","volume":"56","author":"NR Williamson","year":"2005","journal-title":"Mol Microbiol"},{"issue":"Pt 11","key":"pone.0294054.ref007","doi-asserted-by":"crossref","first-page":"3547","DOI":"10.1099\/mic.0.27222-0","article-title":"The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation","volume":"150","author":"AKP Harris","year":"2004","journal-title":"Microbiol Read Engl"},{"issue":"4","key":"pone.0294054.ref008","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1007\/s00253-018-09611-z","article-title":"Recent advancements in high-level synthesis of the promising clinical drug, prodigiosin","volume":"103","author":"CH Yip","year":"2019","journal-title":"Appl Microbiol Biotechnol"},{"issue":"9","key":"pone.0294054.ref009","doi-asserted-by":"crossref","first-page":"5393","DOI":"10.1007\/s13197-015-1740-4","article-title":"Prodigiosin and its potential applications","volume":"52","author":"N Darshan","year":"2015","journal-title":"J Food Sci Technol"},{"issue":"10","key":"pone.0294054.ref010","doi-asserted-by":"crossref","first-page":"001","DOI":"10.7324\/JAPS.2014.401001","article-title":"Prodigiosin\u2014an antibacterial red pigment produced by Serratia marcescens IBRL USM 84 associated with a marine sponge Xestospongia testudinaria","volume":"4","author":"D Ibrahim","year":"2014","journal-title":"J Appl Pharm Sci"},{"issue":"1","key":"pone.0294054.ref011","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13036-021-00262-9","article-title":"Biotechnological Activities and Applications of Bacterial Pigments Violacein and Prodigiosin","volume":"15","author":"SY Choi","year":"2021","journal-title":"J Biol Eng"},{"key":"pone.0294054.ref012","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.3389\/fmicb.2017.01483","article-title":"Genomic, Physiologic, and Symbiotic Characterization of Serratia marcescens Strains Isolated from the Mosquito Anopheles stephensi","volume":"8","author":"S Chen","year":"2017","journal-title":"Front Microbiol"},{"issue":"5","key":"pone.0294054.ref013","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/j.drudis.2020.03.017","article-title":"The production and bioactivity of prodigiosin: quo vadis?","volume":"25","author":"SR Lin","year":"2020","journal-title":"Drug Discov Today"},{"key":"pone.0294054.ref014","doi-asserted-by":"crossref","first-page":"107614","DOI":"10.1016\/j.jmgm.2020.107614","article-title":"Molecular dynamics of the membrane interaction and localisation of prodigiosin","volume":"98","author":"A Ravindran","year":"2020","journal-title":"J Mol Graph Model"},{"issue":"6","key":"pone.0294054.ref015","doi-asserted-by":"crossref","first-page":"e0253445","DOI":"10.1371\/journal.pone.0253445","article-title":"Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition","volume":"16","author":"CH Yip","year":"2021","journal-title":"PloS One"},{"issue":"5","key":"pone.0294054.ref016","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1021\/acsabm.1c01187","article-title":"Prodigiosin-Loaded Poly(lactic acid) to Combat the Biofilm-Associated Infections","volume":"5","author":"C Mudenur","year":"2022","journal-title":"ACS Appl Bio Mater"},{"key":"pone.0294054.ref017","doi-asserted-by":"crossref","first-page":"972","DOI":"10.3389\/fmicb.2016.00972","article-title":"Serratia Secondary Metabolite Prodigiosin Inhibits Pseudomonas aeruginosa Biofilm Development by Producing Reactive Oxygen Species that Damage Biological Molecules","volume":"7","author":"\u00d6 Kimyon","year":"2016","journal-title":"Front Microbiol"},{"key":"pone.0294054.ref018","doi-asserted-by":"crossref","first-page":"105368","DOI":"10.1016\/j.tiv.2022.105368","article-title":"Characterization of prodigiosin pigment by Serratia marcescens and the evaluation of its bioactivities","volume":"82","author":"M Tunca Koyun","year":"2022","journal-title":"Toxicol Vitro Int J Publ Assoc BIBRA"},{"issue":"21","key":"pone.0294054.ref019","doi-asserted-by":"crossref","first-page":"5458","DOI":"10.3390\/ijms20215458","article-title":"Targeted Delivery Prodigiosin to Choriocarcinoma by Peptide-Guided Dendrigraft Poly-l-lysines Nanoparticles","volume":"20","author":"K Zhao","year":"2019","journal-title":"Int J Mol Sci"},{"issue":"11","key":"pone.0294054.ref020","doi-asserted-by":"crossref","first-page":"3846","DOI":"10.1128\/AEM.02772-10","article-title":"Generation of multimillion-sequence 16S rRNA gene libraries from complex microbial communities by assembling paired-end illumina reads","volume":"77","author":"AK Bartram","year":"2011","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"pone.0294054.ref021","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1038\/s41467-019-10210-3","article-title":"TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy","volume":"10","author":"JP Meier-Kolthoff","year":"2019","journal-title":"Nat Commun"},{"issue":"1","key":"pone.0294054.ref022","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1093\/bioinformatics\/btl529","article-title":"Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation","volume":"23","author":"I Letunic","year":"2007","journal-title":"Bioinforma Oxf Engl"},{"issue":"1","key":"pone.0294054.ref023","doi-asserted-by":"crossref","first-page":"5114","DOI":"10.1038\/s41467-018-07641-9","article-title":"High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries","volume":"9","author":"C Jain","year":"2018","journal-title":"Nat Commun"},{"issue":"W1","key":"pone.0294054.ref024","doi-asserted-by":"crossref","first-page":"W29","DOI":"10.1093\/nar\/gkab335","article-title":"antiSMASH 6.0: improving cluster detection and comparison capabilities","volume":"49","author":"K Blin","year":"2021","journal-title":"Nucleic Acids Res"},{"issue":"7","key":"pone.0294054.ref025","doi-asserted-by":"crossref","first-page":"3348","DOI":"10.1128\/AAC.00419-13","article-title":"The comprehensive antibiotic resistance database","volume":"57","author":"AG McArthur","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"pone.0294054.ref026","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/S0891-5520(20)30528-6","article-title":"Antimicrobial susceptibility testing of bacteria that grow aerobically","volume":"7","author":"JH Jorgensen","year":"1993","journal-title":"Infect Dis Clin North Am"},{"issue":"4","key":"pone.0294054.ref027","doi-asserted-by":"crossref","first-page":"823","DOI":"10.4314\/ejhs.v32i4.20","article-title":"Toxicity Study and Antibacterial Effects of the Leaves Extracts of Boscia coriacea and Uvaria leptocladon","volume":"32","author":"ST Tseha","year":"2022","journal-title":"Ethiop J Health Sci"},{"issue":"8","key":"pone.0294054.ref028","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1080\/08927014.2013.820825","article-title":"The anti-biofilm potential of pomegranate (Punica granatum L.) extract against human bacterial and fungal pathogens","volume":"29","author":"D Bakkiyaraj","year":"2013","journal-title":"Biofouling"},{"key":"pone.0294054.ref029","first-page":"938324","article-title":"The Effect of an Aqueous Extract of Teucrium polium on Glutathione Homeostasis In Vitro: A Possible Mechanism of Its Hepatoprotectant Action","volume":"2010","author":"S Shtukmaster","year":"2010","journal-title":"Adv Pharmacol Sci"},{"key":"pone.0294054.ref030","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"MM Bradford","year":"1976","journal-title":"Anal Biochem"},{"issue":"2","key":"pone.0294054.ref031","doi-asserted-by":"crossref","first-page":"e0117121","DOI":"10.1128\/Spectrum.01171-21","article-title":"Identification and Verification of the Prodigiosin Biosynthetic Gene Cluster (BGC) in Pseudoalteromonas rubra S4059","volume":"9","author":"X Wang","year":"2021","journal-title":"Microbiol Spectr"},{"key":"pone.0294054.ref032","doi-asserted-by":"crossref","first-page":"705853","DOI":"10.3389\/fmicb.2021.705853","article-title":"Identification of Essential Genes Associated With Prodigiosin Production in Serratia marcescens FZSF02","volume":"12","author":"X Jia","year":"2021","journal-title":"Front Microbiol"},{"issue":"5","key":"pone.0294054.ref033","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1007\/s00284-019-01665-0","article-title":"Phylogenetic Studies on the Prodigiosin Biosynthetic Operon","volume":"76","author":"A Ravindran","year":"2019","journal-title":"Curr Microbiol"},{"issue":"Pt 11","key":"pone.0294054.ref034","doi-asserted-by":"crossref","first-page":"3547","DOI":"10.1099\/mic.0.27222-0","article-title":"The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation","volume":"150","author":"AKP Harris","year":"2004","journal-title":"Microbiol Read Engl"},{"issue":"2","key":"pone.0294054.ref035","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1263\/jbb.101.157","article-title":"Purification and characterization of prodigiosin produced by integrated bioreactor from Serratia sp. KH-95","volume":"101","author":"MJ Song","year":"2006","journal-title":"J Biosci Bioeng"},{"issue":"1","key":"pone.0294054.ref036","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s43141-020-00045-7","article-title":"Optimization of prodigiosin biosynthesis by Serratia marcescens using unconventional bioresources","volume":"18","author":"A Bhagwat","year":"2020","journal-title":"J Genet Eng Biotechnol"},{"issue":"4","key":"pone.0294054.ref037","first-page":"207","article-title":"Extraction of prodigiosin from Serratia marcescens and its application as an antibacterial spray","volume":"5","author":"K A Simrah Fathima","year":"2020","journal-title":"IP Int J Med Microbiol Trop Dis"},{"issue":"4","key":"pone.0294054.ref038","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1007\/s13399-020-00928-2","article-title":"A comprehensive review on recent trends in production, purification, and applications of prodigiosin","volume":"12","author":"T Paul","year":"2022","journal-title":"Biomass Convers Biorefinery"},{"issue":"14","key":"pone.0294054.ref039","doi-asserted-by":"crossref","first-page":"4967","DOI":"10.1128\/AEM.01986-10","article-title":"Exceptional production of both prodigiosin and cycloprodigiosin as major metabolic constituents by a novel marine bacterium, Zooshikella rubidus S1-1","volume":"77","author":"JS Lee","year":"2011","journal-title":"Appl Environ Microbiol"},{"issue":"18","key":"pone.0294054.ref040","first-page":"6030","article-title":"Extraction of the Anticancer and Antimicrobial Agent, Prodigiosin, from Vibrio gazogenes PB1 and Its Identification by 1D and 2D NMR","volume":"27","author":"D Vijay","year":"2022","journal-title":"Mol Basel Switz"},{"issue":"2","key":"pone.0294054.ref041","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/s11274-014-1793-y","article-title":"Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398","volume":"31","author":"JC Lapenda","year":"2015","journal-title":"World J Microbiol Biotechnol"},{"issue":"4","key":"pone.0294054.ref042","doi-asserted-by":"crossref","first-page":"556","DOI":"10.3390\/microorganisms8040556","article-title":"Applications of Prodigiosin Extracted from Marine Red Pigmented Bacteria Zooshikella sp. and Actinomycete Streptomyces sp","volume":"8","author":"C Ramesh","year":"2020","journal-title":"Microorganisms"},{"key":"pone.0294054.ref043","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/978-1-4419-5979-9_20","article-title":"Synthesis of biosurfactants and their advantages to microorganisms and mankind","volume":"672","author":"SS Cameotra","year":"2010","journal-title":"Adv Exp Med Biol"},{"key":"pone.0294054.ref044","doi-asserted-by":"crossref","first-page":"27","DOI":"10.3389\/fmicb.2016.00027","article-title":"Prodigiosin Induces Autolysins in Actively Grown Bacillus subtilis Cells","volume":"7","author":"T Danev\u010di\u010d","year":"2016","journal-title":"Front Microbiol"},{"issue":"1","key":"pone.0294054.ref045","doi-asserted-by":"crossref","first-page":"29","DOI":"10.5352\/JLS.2015.25.1.29","article-title":"Anti-MRSA Properties of Prodigiosin from Serratia sp. PDGS 120915","volume":"25","author":"Keunho Ji","year":"2015","journal-title":"J Life Sci"},{"key":"pone.0294054.ref046","doi-asserted-by":"crossref","first-page":"6637438","DOI":"10.1155\/2021\/6637438","article-title":"Current Understanding on Adhesion and Biofilm Development in Actinobacteria","volume":"2021","author":"R El Othmany","year":"2021","journal-title":"Int J Microbiol"},{"issue":"22","key":"pone.0294054.ref047","doi-asserted-by":"crossref","first-page":"6137","DOI":"10.1021\/acs.langmuir.5b00815","article-title":"Role of Flagella in Adhesion of Escherichia coli to Abiotic Surfaces","volume":"31","author":"RS Friedlander","year":"2015","journal-title":"Langmuir ACS J Surf Colloids"},{"key":"pone.0294054.ref048","doi-asserted-by":"crossref","first-page":"178","DOI":"10.3389\/fcimb.2014.00178","article-title":"Staphylococcus aureus biofilms: recent developments in biofilm dispersal","volume":"4","author":"JL Lister","year":"2014","journal-title":"Front Cell Infect Microbiol"},{"issue":"6","key":"pone.0294054.ref049","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1134\/S0026261722010052","article-title":"Prodigiosin from an Endofungal Bacterium Serratia marcescens D1 Inhibits Biofilm Formation in Gram-Positive Bacteria","volume":"90","author":"DJ Hazarika","year":"2021","journal-title":"Microbiology"},{"issue":"5","key":"pone.0294054.ref050","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.3390\/antiox12051094","article-title":"How to Increase Cellular Glutathione","volume":"12","author":"D Giustarini","year":"2023","journal-title":"Antioxid Basel Switz"},{"issue":"5","key":"pone.0294054.ref051","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1080\/14786419.2016.1195380","article-title":"Antimicrobial activity of prodigiosin is attributable to plasma-membrane damage","volume":"31","author":"RK Suryawanshi","year":"2017","journal-title":"Nat Prod Res"},{"key":"pone.0294054.ref052","doi-asserted-by":"crossref","first-page":"7374316","DOI":"10.1155\/2016\/7374316","article-title":"Protein Quantification by Derivatization-Free High-Performance Liquid Chromatography of Aromatic Amino Acids","volume":"2016","author":"A Hesse","year":"2016","journal-title":"J Amino Acids"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0294054","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T18:40:25Z","timestamp":1700073625000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0294054"}},"subtitle":[],"editor":[{"given":"Marcos","family":"Pileggi","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,11,15]]},"references-count":52,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,11,15]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0294054","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,15]]}}}