{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:15:46Z","timestamp":1781100946252,"version":"3.54.1"},"reference-count":24,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>After issuing the \u201cGlobal action plan on antimicrobial resistance\u201d in 2015, the World Health Organization (WHO) established a priority pathogens list for supporting research and development of novel antimicrobials. We conducted a comprehensive analysis of the WHO priority organisms in a Japanese tertiary hospital to apprehend the local AMR epidemiology.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Data were obtained from electrical medical records in Osaka University Hospital between January 2010 and March 2021. The critical, high, and medium \u201cpriority pathogens list\u201d categories of the WHO were used to compare results between the early (2010\u20132015) and late (2016\u20132021) phases.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Out of 52,130 culture-positive specimens, a total of 9,872 (18.9%) contained WHO priority isolates. In comparison to early phases, late phases were likely to have higher rates of carbapenem resistance in <jats:italic>Pseudomonas aeruginosa<\/jats:italic> (15.7% vs 25.0%, P&lt;0.001), 3<jats:sup>rd<\/jats:sup> generation cephalosporin resistance in <jats:italic>Escherichia coli<\/jats:italic> (11.5% vs 17.8%, P&lt;0.001) as well as <jats:italic>Klebsiella pneumoniae<\/jats:italic> (1.6% vs 4.4%, P&lt;0.001), and ampicillin resistance in <jats:italic>Haemophilus influenzae<\/jats:italic> (2.4% vs 3.9%, P&lt;0.001). After 2015, however, the proportion of methicillin-resistant and vancomycin-intermediate <jats:italic>Staphylococcus aureus<\/jats:italic> was low. In this study, in-hospital mortality was comparable among patients with resistance to the three WHO priority pathogen types: critical (5.9%), high (3.9%), and medium (3.8%), and no significant change was observed between two phases in each category. However, significant interactions for in-hospital mortality were observed in subgroup analyses between \u201ccritical priority\u201d AMR and the presence of comorbid conditions, such as chronic kidney disease or diabetes mellitus.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>To implement better antimicrobial stewardship policies and practices, local priority pathogens and \u201chigh-risk\u201d patients for in-hospital death need to be acknowledged and evaluated periodically.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0294229","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T18:26:46Z","timestamp":1704997606000},"page":"e0294229","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Antimicrobial resistance patterns of WHO priority pathogens isolated in hospitalized patients in Japan: A tertiary center observational study"],"prefix":"10.1371","volume":"19","author":[{"given":"Tania Tabassum","family":"Nisa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1534-2033","authenticated-orcid":true,"given":"Daisaku","family":"Nakatani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumie","family":"Kaneko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Takeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Nakata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,1,11]]},"reference":[{"key":"pone.0294229.ref001","doi-asserted-by":"crossref","first-page":"134","DOI":"10.3389\/fmicb.2010.00134","article-title":"A brief history of the antibiotic era: lessons learned and challenges for the future","volume":"1","author":"RI Aminov","year":"2010","journal-title":"Front Microbiol"},{"key":"pone.0294229.ref002","article-title":"Antimicrobial resistance 2021, November 17","author":"World Health Organization"},{"issue":"145","key":"pone.0294229.ref003","article-title":"The Antimicrobial Resistance Crisis: Causes, Consequences, and Management.","volume":"2","author":"CA Michael","year":"2014","journal-title":"Frontiers in Public Health"},{"key":"pone.0294229.ref004","author":"Review on Antimicrobial Resistance","year":"2016","journal-title":"Tackling Drug-Resistant Infections Globally: final report and recommendations"},{"key":"pone.0294229.ref005","article-title":"New report calls for urgent action to avert antimicrobial resistance crisis","author":"World Health Organization","year":"2019"},{"key":"pone.0294229.ref006","article-title":"Antimicrobial Resistance: Tackling a Crisis for the Future Health and Wealth of Nations","author":"Review on Antimicrobial Resistance","year":"2014"},{"key":"pone.0294229.ref007","article-title":"Global action plan on antimicrobial resistance","author":"World Health Organization","year":"2015"},{"issue":"3","key":"pone.0294229.ref008","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","article-title":"Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis","volume":"18","author":"E Tacconelli","year":"2018","journal-title":"Lancet Infect Dis"},{"key":"pone.0294229.ref009","article-title":"Prioritization of pathogens to guide discovery, research and development of new antibiotics for drug resistant bacterial infections, including tuberculosis 2017","author":"World Health Organization"},{"key":"pone.0294229.ref010","article-title":"WHO publishes list of bacteria for which new antibiotics are urgently needed.","author":"World Health Organization","year":"2017"},{"key":"pone.0294229.ref011","article-title":"Japan: National action plan on antimicrobial resistance (AMR).","author":"World Health Organization","year":"2016"},{"key":"pone.0294229.ref012","article-title":"Nippon AMR One Health Report (NAOR) 2017 October 18, 2017","author":"The Ministry of Health LaW, The AMR One Health Surveillance Committee"},{"key":"pone.0294229.ref013","article-title":"Nippon AMR One Health Report (NAOR) 2018 November 29, 2018","author":"The Ministry of Health LaW, The AMR One Health Surveillance Committee"},{"key":"pone.0294229.ref014","article-title":"Nippon AMR One Health Report (NAOR) 2019 November 27, 2019","author":"The Ministry of Health Law, The AMR One Health Surveillance Committee"},{"key":"pone.0294229.ref015","article-title":"Nippon AMR One Health Report (NAOR) 2020 January 8, 2021","author":"The Ministry of Health Law, The AMR One Health Surveillance Committee"},{"key":"pone.0294229.ref016","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1471-2288-11-40","article-title":"Opt-out as an acceptable method of obtaining consent in medical research: a short report.","volume":"11","author":"A Vellinga","year":"2011","journal-title":"BMC Med Res Methodol"},{"key":"pone.0294229.ref017","unstructured":"Recommendations RoARTD-RIGfra. 2016, May19 https:\/\/amrreview.org\/sites\/default\/files\/160525_Final%20paper_with%20cover.pdf Accessed 2021 December 29."},{"issue":"1","key":"pone.0294229.ref018","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1186\/s12913-018-3604-x","article-title":"Japan nosocomial infections surveillance (JANIS): a model of sustainable national antimicrobial resistance surveillance based on hospital diagnostic microbiology laboratories","volume":"18","author":"A Tsutsui","year":"2018","journal-title":"BMC Health Serv Res"},{"key":"pone.0294229.ref019","article-title":"Pseudomonas aeruginosa in Healthcare Settings","author":"Prevention CfDCa"},{"issue":"4","key":"pone.0294229.ref020","first-page":"303","article-title":"Mechanisms responsible for the emergence of carbapenem resistance in Pseudomonas aeruginosa.","volume":"16","author":"G Meletis","year":"2012","journal-title":"Hippokratia"},{"issue":"10","key":"pone.0294229.ref021","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/jac\/dkw216","article-title":"Colonization with third-generation cephalosporin-resistant Enterobacteriaceae on hospital admission: prevalence and risk factors","volume":"71","author":"A Hamprecht","year":"2016","journal-title":"Journal of Antimicrobial Chemotherapy"},{"issue":"Suppl S1","key":"pone.0294229.ref022","first-page":"2","article-title":"Overall immune profile and effect of chronic kidney disease on vaccination schedule","volume":"26","year":"2016","journal-title":"Indian Journal of Nephrology"},{"issue":"1","key":"pone.0294229.ref023","doi-asserted-by":"crossref","first-page":"13372","DOI":"10.1038\/s41598-018-31612-1","article-title":"Association of Kidney Function with Infections by Multidrug-Resistant Organisms: An Electronic Medical Record Analysis","volume":"8","author":"XH Su G","year":"2018","journal-title":"Scientific reports"},{"issue":"5","key":"pone.0294229.ref024","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1007\/s00203-020-01818-x","article-title":"Diabetes-associated infections: development of antimicrobial resistance and possible treatment strategies","volume":"202","author":"MSH Akash","year":"2020","journal-title":"Arch Microbiol"}],"container-title":["PLOS 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