{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T04:52:18Z","timestamp":1746852738373,"version":"3.37.3"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T00:00:00Z","timestamp":1699920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001922","name":"Efficacy and Mechanism Evaluation Programme","doi-asserted-by":"publisher","award":["project reference 13\/53\/03"],"award-info":[{"award-number":["project reference 13\/53\/03"]}],"id":[{"id":"10.13039\/501100001922","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"\nBackground<\/jats:title>\nAgeing is associated with changes in body composition including an overall reduction in muscle mass and a proportionate increase in fat mass. Sarcopenia is characterised by losses in both muscle mass and strength. Body composition and muscle strength are at least in part genetically determined, consequently polymorphisms in pathways important in muscle biology (e.g., the activin\/myostatin signalling pathway) are hypothesised to contribute to the development of sarcopenia.<\/jats:p>\n<\/jats:sec>\n\nMethods<\/jats:title>\nWe compared regional body composition measured by DXA with genotypes for two polymorphisms (rs10783486, minor allele frequency (MAF) = 0.26 and rs2854464, MAF = 0.26) in the activin 1B receptor (ACVR1B<\/jats:italic>) determined by PCR in a cross-sectional analysis of DNA from 110 older individuals with sarcopenia from the LACE trial.<\/jats:p>\n<\/jats:sec>\n\nResults<\/jats:title>\nNeither muscle mass nor strength showed any significant associations with either genotype in this cohort. Initial analysis of rs10783486 showed that males with the AA\/AG genotype were taller than GG males (174\u00b17cm vs 170\u00b15cm, p = 0.023) and had higher arm fat mass, (median higher by 15%, p = 0.008), and leg fat mass (median higher by 14%, p = 0.042). After correcting for height, arm fat mass remained significantly higher (median higher by 4% padj<\/jats:sub> = 0.024). No associations (adjusted or unadjusted) were seen in females. Similar analysis of the rs2854464 allele showed a similar pattern with the presence of the minor allele (GG\/AG) being associated with greater height (GG\/AG = 174\u00b17 cm vs AA = 170 \u00b15cm, p = 0.017) and greater arm fat mass (median higher by 16%, p = 0.023). Again, the difference in arm fat remained after correction for height. No similar associations were seen in females analysed alone.<\/jats:p>\n<\/jats:sec>\n\nConclusion<\/jats:title>\nThese data suggest that polymorphic variation in the ACVR1B<\/jats:italic> locus could be associated with body composition in older males. The activin\/myostatin pathway might offer a novel potential target to prevent fat accumulation in older individuals.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0294330","type":"journal-article","created":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T18:24:35Z","timestamp":1699986275000},"page":"e0294330","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Activin type I receptor polymorphisms and body composition in older individuals with sarcopenia\u2014Analyses from the LACE randomised controlled 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Voisin","year":"2016","journal-title":"PLoS One"},{"issue":"12","key":"pone.0294330.ref023","doi-asserted-by":"crossref","DOI":"10.3390\/genes11121459","article-title":"The Association of Multiple Gene Variants with Ageing Skeletal Muscle Phenotypes in Elderly Women","volume":"11","author":"P Khanal","year":"2020","journal-title":"Genes (Basel)"},{"issue":"8","key":"pone.0294330.ref024","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1249\/MSS.0b013e31828983f7","article-title":"Genetic predisposition scores associate with muscular strength, size, and trainability","volume":"45","author":"T Thomaes","year":"2013","journal-title":"Med Sci Sports Exerc"},{"issue":"9","key":"pone.0294330.ref025","doi-asserted-by":"crossref","first-page":"e0275179","DOI":"10.1371\/journal.pone.0275179","article-title":"Genetic polymorphisms of muscular fitness in young healthy men","volume":"17","author":"T Venckunas","year":"2022","journal-title":"PLoS 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Muscle"},{"issue":"1","key":"pone.0294330.ref032","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s13063-017-2390-9","article-title":"Leucine and ACE inhibitors as therapies for sarcopenia (LACE trial): study protocol for a randomised controlled trial","volume":"19","author":"MM Band","year":"2018","journal-title":"Trials"},{"issue":"1","key":"pone.0294330.ref033","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1093\/ije\/dyx206","article-title":"Collider scope: when selection bias can substantially influence observed associations","volume":"47","author":"MR Munafo","year":"2018","journal-title":"Int J Epidemiol"},{"issue":"1","key":"pone.0294330.ref034","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s00421-011-1975-3","article-title":"Maximal isokinetic and isometric muscle strength of major muscle groups related to age, body mass, height, and sex in 178 healthy subjects","volume":"112","author":"T Harbo","year":"2012","journal-title":"Eur J 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