{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T03:24:22Z","timestamp":1765423462628,"version":"3.40.5"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:00:00Z","timestamp":1700092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CHU de Besan\u00e7on","award":["API\/2018\/99"],"award-info":[{"award-number":["API\/2018\/99"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Antimicrobial resistance is a global health issue and extended-spectrum \u03b2-lactamase producing <jats:italic>Escherichia coli<\/jats:italic> (ESBL-Ec) and methicillin-resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) are of particular concern. Whole genome sequencing analysis of isolates from the community is essential to understand the circulation of those multidrug-resistant bacteria. Our main objective was to determine the population structure of clinical ESBL-Ec and MRSA isolated in the community setting of a French region. For this purpose, isolates were collected from 23 sites belonging to 6 private medical biology laboratories in the Bourgogne-Franche-Comt\u00e9 region. One hundred ninety ESBL-Ec and 67 MRSA were sequenced using the Illumina technology. Genomic analyses were performed to determine the bacterial typing, presence of antibiotic resistance genes, metal resistance genes as well as virulence genes. Analysis showed that ST131 was the major ESBL-Ec clone circulating in the region, representing 42.1% of the ESBL-Ec isolates. The <jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub> genes represented 98% of <jats:italic>bla<\/jats:italic><jats:sub>ESBL<\/jats:sub> with the majority being <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub> (53.9%). MRSA population consisted of mainly of CC8 (50.7%) and CC5 (38.8%) clonal complexes. Interestingly, we found a prevalence of 40% of the zinc resistance gene <jats:italic>czrC<\/jats:italic> in our MRSA population. We observed no differences in our ESBL-Ec or MRSA populations between urban and rural areas in our French region, suggesting no impact of population density or rural environment.<\/jats:p>","DOI":"10.1371\/journal.pone.0294433","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T18:23:06Z","timestamp":1700158986000},"page":"e0294433","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Population structure of community-acquired extended-spectrum beta-lactamase producing Escherichia coli and methicillin resistant Staphylococcus aureus in a French region showed no difference between urban and rural areas"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7036-8922","authenticated-orcid":true,"given":"Adrien","family":"Biguenet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier","family":"Bertrand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilou","family":"Bourgeon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dossi Carine","family":"Gnide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houssein","family":"Gbaguidi-Haore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9line","family":"Slekovec","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,11,16]]},"reference":[{"key":"pone.0294433.ref001","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s13756-018-0336-y","article-title":"Estimating the burden of antimicrobial resistance: a systematic literature review","volume":"7","author":"NR Naylor","year":"2018","journal-title":"Antimicrob Resist Infect Control"},{"issue":"1","key":"pone.0294433.ref002","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/S1473-3099(18)30605-4","article-title":"Attributable deaths and disability-adjusted life-years caused by infections with antibiotic-resistant bacteria in the EU and the European Economic Area in 2015: a population-level modelling analysis","volume":"19","author":"A Cassini","year":"2019","journal-title":"Lancet Infect Dis"},{"issue":"4","key":"pone.0294433.ref003","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1136\/jcp.14.4.385","article-title":"Methicillin-resistant staphylococci","volume":"14","author":"M. 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