{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:47:33Z","timestamp":1787510853790,"version":"build-2736575974"},"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Cotton crop yields are largely affected by infestations of\n                    <jats:italic>Anthonomus grandis<\/jats:italic>\n                    , which is its main pest. Although\n                    <jats:italic>Bacillus thuringiensis<\/jats:italic>\n                    (Bt) derived proteins can limit insect pest infestations, the diverse use of control methods becomes a viable alternative in order to prolong the use of technology in the field. One of the alternative methods to Bt technology has been the utilization of certain\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    species highly efficient in controlling coleopteran insects have been used to produce highly toxic insecticidal proteins. This study aimed to evaluate the toxicity of IPD072Aa and PIP-47Aa proteins, isolated from\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    spp., in interaction with Cry1Ia10, Cry3Aa, and Cry8B proteins isolated from\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>thuringiensis<\/jats:italic>\n                    , to control\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>grandis<\/jats:italic>\n                    in cotton crops. The genes IPD072Aa and PIP-47Aa were synthesized and cloned into a pET-SUMO expression vector. Moreover, Cry1Ia10, Cry3Aa, and Cry8B proteins were obtained by inducing recombinant\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>coli<\/jats:italic>\n                    clones, which were previously acquired by our research group from the Laboratory of Bacteria Genetics and Applied Biotechnology (LGBBA). These proteins were visualized in SDS-PAGE, quantified, and incorporated into an artificial diet to estimate their lethal concentrations (LC) through individual or combined bioassays. The results of individual toxicity revealed that IPD072Aa, PIP-47Aa, Cry1Ia10, Cry3Aa, and Cry8B were efficient in controlling\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>grandis<\/jats:italic>\n                    , with the latter being the most toxic. Regarding interaction assays, a high synergistic interaction was observed between Cry1Ia10 and Cry3Aa. All interactions involving Cry3Aa and PIP-47Aa, when combined with other proteins, showed a clear synergistic effect. Our findings highlighted that the tested proteins in combination, for the most part, increase toxicity against\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>grandis<\/jats:italic>\n                    neonate larvae, suggesting possible constructions for pyramiding cotton plants to the manage and the control boll weevils.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0294654","type":"journal-article","created":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T14:05:45Z","timestamp":1701353145000},"page":"e0294654","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Interaction of insecticidal proteins from Pseudomonas spp. and Bacillus thuringiensis for boll weevil management"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8354-8463","authenticated-orcid":true,"given":"Jardel Diego","family":"Barbosa Rodrigues","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4253-0320","authenticated-orcid":true,"given":"Raquel Oliveira","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackson Ant\u00f4nio Marcondes","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janete Apparecida","family":"Desid\u00e9rio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,11,30]]},"reference":[{"key":"pone.0294654.ref001","unstructured":"ICAC\u2014International Cotton Advisory Committee. 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