{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:45:46Z","timestamp":1791387946396,"version":"4.3.3"},"reference-count":67,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T00:00:00Z","timestamp":1707091200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005317","name":"Kermanshah University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005317","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Increasing illicit drug use is one of the main problems in most countries or societies. Monitoring heavy metals and trace elements in this vulnerable group seems to be necessary. Therefore, we assessed the urinary trace element and toxic metals\/metalloids concentrations (Zinc (Zn), Iron (Fe), Copper (Cu), Chromium (Cr), Lead (Pb), Cadmium (Cd), Arsenic (As), Nickel (Ni), and Mercury (Hg)) in opium, tramadol, and cannabis users compared to healthy subjects. In this cross-sectional study, patients with substance use disorder (SUD) (n = 74) were divided into four groups: cannabis, tramadol, opium, and mixed (simultaneous use of more than one of the three studied substances), along with a healthy group (n = 60). Urine samples were prepared by dispersive liquid-liquid microextraction method so that heavy metals\/metalloids could be measured by ICP-MS. The mean urinary concentration of Cu (48.15 vs. 25.45; 89.2%, p&lt;0.001), Hg (1.3 vs. 0.10; 1200%, <jats:italic>p<\/jats:italic> &lt; 0.001), and Zn (301.95 vs. 210; 43.8%, <jats:italic>p<\/jats:italic> &lt; 0.001) was markedly lower among patients with SUD. The mean urinary concentration of other elements including As (1.9 vs. 4.1; 115.8%), Cd (0.1 vs. 1.10; 1000%), Cr (6.80 vs. 11.65; 71.3%), Ni (2.95 vs. 4.95; 67.8%), and Pb (1.5 vs. 7.9; 426.6%) were significantly higher among patients with SUD compared to healthy subjects. When sub-groups were compared, no significant differences were observed between their trace element levels (Kruskal-Wallis test, <jats:italic>p<\/jats:italic> &gt; 0.05). This can be an indication that regardless of the type of drug, the levels of trace elements are changed with respect to healthy individuals. Our results showed that illicit drug use causes changes in urinary trace element\/heavy metal\/metalloid levels and highlights the need for monitoring heavy metals and trace elements in individuals with substance use disorder. Assessment of different elements in biological samples of drug dependents may be useful for implementing new prevention and treatment protocols. In case of changes in their levels, complementary recommendations, attention to diet, and periodic assessment of toxic metal levels within treatment programs will be needed.<\/jats:p>","DOI":"10.1371\/journal.pone.0294740","type":"journal-article","created":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T18:27:00Z","timestamp":1707157620000},"page":"e0294740","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Trace element levels: How Substance Use Disorder (SUD) contributes to the alteration of urinary essential and toxic element levels"],"prefix":"10.1371","volume":"19","author":[{"given":"Borhan","family":"Mansouri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nammamali","family":"Azadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arezo 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non-cancerous tissues with the risk of gastrointestinal cancers in Eastern Iran","volume":"28","author":"F Nozadi","year":"2021","journal-title":"Environmental Science and Pollution Research"},{"issue":"1","key":"pone.0294740.ref012","first-page":"3018","article-title":"Health issues and heavy metals","volume":"3","author":"P Bhargava","year":"2017","journal-title":"Austin J Environ Toxicol"},{"issue":"35","key":"pone.0294740.ref013","doi-asserted-by":"crossref","first-page":"35787","DOI":"10.1007\/s11356-019-06632-7","article-title":"Thyroid dysfunction: how concentration of toxic and essential elements contribute to risk of hypothyroidism, hyperthyroidism, and thyroid cancer","volume":"26","author":"M Rezaei","year":"2019","journal-title":"Environmental Science and Pollution Research"},{"issue":"3","key":"pone.0294740.ref014","doi-asserted-by":"crossref","first-page":"101865","DOI":"10.1016\/j.jksus.2022.101865","article-title":"Impact of heavy metals on the environment and human 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Organization"},{"key":"pone.0294740.ref021","author":"H Salehi","year":"2009","journal-title":"Comparison of serum lead level in oral opium addicts with healthy control group"},{"key":"pone.0294740.ref022","first-page":"1","article-title":"Comparison of urine trace element levels in tramadol addiction alone and its co-abuse with cigarette and opium in Western Iran","author":"V Farnia","year":"2022","journal-title":"Environmental Science and Pollution Research"},{"issue":"2","key":"pone.0294740.ref023","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1159\/000338559","article-title":"Opium could be considered an independent risk factor for lung cancer: a case-control study","volume":"85","author":"MR Masjedi","year":"2013","journal-title":"Respiration"},{"issue":"2","key":"pone.0294740.ref024","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/s11419-020-00569-0","article-title":"A review on tramadol toxicity: mechanism of action, clinical presentation, and treatment","volume":"39","author":"S Nakhaee","year":"2021","journal-title":"Forensic Toxicology"},{"issue":"12","key":"pone.0294740.ref025","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1111\/add.15059","article-title":"Tramadol use and public health consequences in Iran: a systematic review and meta\u2010analysis","volume":"115","author":"Y Rostam\u2010Abadi","year":"2020","journal-title":"Addiction"},{"key":"pone.0294740.ref026","doi-asserted-by":"crossref","DOI":"10.1186\/2008-2231-20-8","volume-title":"Chronic lead poisoning in Iran; a silent disease","author":"O Mehrpour","year":"2012"},{"issue":"1","key":"pone.0294740.ref027","first-page":"25","article-title":"Effect of tramadol addiction alone and its co-abuse with cannabis on urinary excretion of Copper, Zinc, and Calcium among Egyptian addicts. Catrina","volume":"17","author":"I El-Safty","year":"2018","journal-title":"The International Journal of Environmental Sciences"},{"issue":"2","key":"pone.0294740.ref028","doi-asserted-by":"crossref","first-page":"52","DOI":"10.32441\/jaset.03.02.06","article-title":"Investigation on Glucose and levels of Zn and Cu in Sera of Iraqi Males addicted on Methamphetamine or Tramadol","volume":"3","author":"R Sabah","year":"2020","journal-title":"Journal of Advanced Sciences and Engineering Technologies"},{"key":"pone.0294740.ref029","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.jes.2019.04.028","article-title":"Pesticides and trace elements in cannabis: Analytical and environmental challenges and opportunities","volume":"85","author":"CB Craven","year":"2019","journal-title":"Journal of Environmental Sciences"},{"key":"pone.0294740.ref030","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1007\/978-3-319-54564-6_22","volume-title":"Cannabis sativa L-Botany and 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Yang","year":"2018","journal-title":"Toxicology and applied pharmacology"},{"key":"pone.0294740.ref034","doi-asserted-by":"crossref","first-page":"708069","DOI":"10.3389\/fnut.2021.708069","article-title":"Excretion of heavy metals and glyphosate in urine and hair before and after long-term fasting in humans","volume":"8","author":"F Grundler","year":"2021","journal-title":"Frontiers in Nutrition"},{"issue":"3","key":"pone.0294740.ref035","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1080\/03067319.2016.1150464","article-title":"Application of microwave-assisted dispersive liquid\u2013liquid microextraction and graphite furnace atomic absorption spectrometry for ultra-trace determination of lead and cadmium in cereals and agricultural products","volume":"96","author":"M Ataee","year":"2016","journal-title":"International journal of environmental analytical chemistry"},{"issue":"9","key":"pone.0294740.ref036","doi-asserted-by":"crossref","first-page":"24553","DOI":"10.1007\/s11356-022-23942-5","article-title":"Evaluation of blood lead levels in opium addicts and healthy control group using novel deep eutectic solvent based dispersive liquid\u2013liquid microextraction followed by GFAAS","volume":"30","author":"TA Jouybari","year":"2023","journal-title":"Environmental Science and Pollution Research"},{"issue":"6","key":"pone.0294740.ref037","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1093\/nutrit\/nuaa095","article-title":"Nutritional status and eating habits of people who use drugs and\/or are undergoing treatment for recovery: a narrative review","volume":"79","author":"N Mahboub","year":"2021","journal-title":"Nutrition reviews"},{"key":"pone.0294740.ref038","article-title":"Diet and nutrient intake of people receiving opioid agonist treatment (OAT): implications for recovery","author":"SS Ii","year":"2016","journal-title":"Drugs and Alcohol Today"},{"issue":"4","key":"pone.0294740.ref039","first-page":"339","article-title":"Nutritional status of opiate-dependent persons before and during methadone maintenance therapy","volume":"61","author":"A Szpanowska-Wohn","year":"2004","journal-title":"Przeglad lekarski"},{"key":"pone.0294740.ref040","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1096\/fasebj.23.1_supplement.736.19","article-title":"Nutritional status of methadone\u2010treated pregnant women","volume":"23","author":"LE Tomedi","year":"2009","journal-title":"The FASEB Journal"},{"issue":"4","key":"pone.0294740.ref041","first-page":"332","article-title":"Intake of nutrients in daily nutritional ratios by opiate dependent persons during methadone maintenance therapy","volume":"61","author":"A Szpanowska-Wohn","year":"2004","journal-title":"Przeglad lekarski"},{"issue":"319","key":"pone.0294740.ref042","first-page":"2","article-title":"Comparison of Nutritional Status and Food Insecurity among People Who Inject Illicit Drugs, Non Drug Users and Those on Methadone Treatment in Selected Areas of Nairobi, Kenya","volume":"6","author":"V Wanjihia","year":"2018","journal-title":"J Alcohol Drug Depend"},{"issue":"2","key":"pone.0294740.ref043","doi-asserted-by":"crossref","first-page":"125","DOI":"10.5114\/wo.2012.28792","article-title":"The impact of opioid analgesics on the gastrointestinal tract function and the current management possibilities [Polish version: Wp\u0142yw opioidowych \u015brodk\u00f3w przeciwb\u00f3lowych na czynno\u015b\u0107 uk\u0142adu pokarmowego oraz aktualne mo\u017cliwo\u015bci post\u0119powania terapeutycznego p. 132]","volume":"16","author":"W. Leppert","year":"2012","journal-title":"Contemporary Oncology\/Wsp\u00f3\u0142czesna Onkologia"},{"issue":"1","key":"pone.0294740.ref044","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s12011-010-8634-0","article-title":"Antioxidant enzyme and element status in heroin addiction or heroin withdrawal in rats: effect of melatonin and vitamin E plus Se","volume":"139","author":"M Cemek","year":"2011","journal-title":"Biological trace element research"},{"issue":"10","key":"pone.0294740.ref045","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/BF01648242","article-title":"Trace elements in drug addicts","volume":"68","author":"MR Mart\u00ednez","year":"1990","journal-title":"Klinische Wochenschrift"},{"issue":"2","key":"pone.0294740.ref046","doi-asserted-by":"crossref","first-page":"145","DOI":"10.5055\/jom.2021.0607","article-title":"Zinc deficiency: A cause of opioid-induced physical dependence and addiction in post-operative total hip arthroplasty patients","volume":"17","author":"TJ Tantillo","year":"2021","journal-title":"Journal of Opioid Management"},{"issue":"10","key":"pone.0294740.ref047","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1002\/jnr.23609","article-title":"Enhanced analgesic effects of tramadol and common trace element coadministration in mice","volume":"93","author":"T Alexa","year":"2015","journal-title":"Journal of neuroscience research"},{"key":"pone.0294740.ref048","first-page":"1","article-title":"Multivariate statistical evaluation of heavy metals in the urine of opium individuals in comparison with healthy people in Western Iran","author":"N Azadi","year":"2021","journal-title":"Environmental Science and Pollution Research"},{"issue":"1","key":"pone.0294740.ref049","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s40199-018-0205-5","article-title":"Occurrence of bacterial and toxic metals contamination in illegal opioid-like drugs in Iran: a significant health challenge in drug abusers","volume":"26","author":"R Aghababaei","year":"2018","journal-title":"DARU Journal of Pharmaceutical Sciences"},{"issue":"6","key":"pone.0294740.ref050","doi-asserted-by":"crossref","first-page":"282","DOI":"10.17221\/3442-PSE","article-title":"Relations among alkaloids, cadmium and zinc contents in opium poppy (Papaver somniferum L.)","volume":"52","author":"J Lachman","year":"2006","journal-title":"Plant Soil and Environment"},{"key":"pone.0294740.ref051","first-page":"226","article-title":"Determination of cadmium in poppy seeds and in poppy seeds containing products. Book of abstract","author":"J Knapek","year":"2009","journal-title":"4th international symposium on recent advances in food analysis"},{"issue":"7","key":"pone.0294740.ref052","article-title":"Content of Cadmium in Poppy Seeds and Poppy Seeds Containing Products Marketed in Czech Republic","volume":"5","author":"J Knapek","year":"2011","journal-title":"Journal of Environmental Science and Engineering"},{"key":"pone.0294740.ref053","first-page":"496","article-title":"Content of heavy metals in poppy seeds (Papaver somniferum L.)","author":"S Ivan","year":"2011","journal-title":"Advances in Environmental Biology"},{"issue":"5","key":"pone.0294740.ref054","first-page":"553","article-title":"Presence of lead in opium","volume":"11","author":"M Aghaee-Afshar","year":"2008","journal-title":"Arch Iran Med"},{"issue":"5","key":"pone.0294740.ref055","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1093\/jaoac\/88.5.1469","article-title":"Quantitative evaluation of 28 mineral elements by inductively coupled plasma\/mass spectrometry and its application in source identification of Indian opium","volume":"88","author":"MR Mudiam","year":"2005","journal-title":"Journal of AOAC International"},{"issue":"8","key":"pone.0294740.ref056","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1177\/0960327115607947","article-title":"Comparison of serum and urine lead levels in opium addicts with healthy control group","volume":"35","author":"H Khatibi-Moghadam","year":"2016","journal-title":"Human & experimental toxicology"},{"issue":"10","key":"pone.0294740.ref057","first-page":"939","article-title":"Blood lead level in opium dependents and its association with anemia: A cross-sectional study from the capital of Iran","volume":"19","author":"BH Domeneh","year":"2014","journal-title":"Journal of research in medical sciences: the official journal of Isfahan University of Medical Sciences"},{"issue":"1","key":"pone.0294740.ref058","first-page":"72","article-title":"Abdominal pain due to lead-contaminated opium: a new source of inorganic lead poisoning","volume":"9","author":"M Mohsen Masoodi","year":"2006","journal-title":"Iran. Arch Iran Med"},{"key":"pone.0294740.ref059","first-page":"1","article-title":"Risk assessment of elevated blood lead concentrations in the adult population using a decision tree approach","author":"A Amirabadizadeh","year":"2020","journal-title":"Drug and Chemical Toxicology"},{"issue":"1","key":"pone.0294740.ref060","article-title":"Blood lead levels in asymptomatic opium addict patients; a case control study","volume":"5","author":"K Ghaemi","year":"2017","journal-title":"Emergency"},{"issue":"4","key":"pone.0294740.ref061","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1080\/17425255.2019.1588250","article-title":"The transition of lead and microbial contamination from adulterated opium to the human body","volume":"15","author":"S Nakhaee","year":"2019","journal-title":"Expert opinion on drug metabolism & toxicology"},{"key":"pone.0294740.ref062","doi-asserted-by":"crossref","first-page":"126495","DOI":"10.1016\/j.jtemb.2020.126495","article-title":"Morphine-element interactions\u2013The influence of selected chemical elements on neural pathways associated with addiction","volume":"60","author":"P Kupnicka","year":"2020","journal-title":"Journal of Trace Elements in Medicine and Biology"},{"issue":"3","key":"pone.0294740.ref063","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S0091-3057(02)00729-3","article-title":"The effects of developmental cadmium exposure on morphine sensitization and challenge with selective D1 and D2 antagonists","volume":"72","author":"KR Smith","year":"2002","journal-title":"Pharmacology Biochemistry and Behavior"},{"issue":"1","key":"pone.0294740.ref064","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1023\/A:1009286709935","article-title":"Effects of subchronic alternating cadmium exposure on dopamine turnover and plasma levels of prolactin","volume":"13","author":"A Lafuente","year":"2000","journal-title":"GH and ACTH. 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