{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T21:52:58Z","timestamp":1793137978520,"version":"4.5.0"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000272","name":"NIHR","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000272","name":"NIHR","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Institute for Health Research Capability Fund from Norfolk and Norwich University Hospital"},{"name":"Norfolk Heart Trust"},{"name":"B Braun Ltd."}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Aim<\/jats:title>\n<jats:p>Inflammation plays a central role in the pathogenesis of atherosclerosis and in the sequelae of percutaneous coronary intervention (PCI). Previous work demonstrated that intermediate monocytes (CD14++CD16+) are associated with adverse cardiovascular events, yet monocyte subset response following elective PCI has not been described. This article explores the changes in monocyte subset and humoral response after elective PCI.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>This prospective study included 30 patients without inflammatory diseases being referred for elective PCI. We included patients treated with drug coated balloons or 2<jats:sup>nd<\/jats:sup> generation drug eluting stents. Patients underwent blood tests at baseline (prior to PCI), four hours, two weeks and two months later. Analyses were performed in terms of monocyte subsets (classical CD14++CD16-, intermediate CD14++CD16+ and non-classical CD14+CD16++), gene expression of CD14+ leucocytes and humoral biomarkers.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Intermediate monocytes decreased significantly four hours after PCI, were recovered at two weeks, and increased significantly at two months post elective, uncomplicated PCI. They remain significantly elevated in the DES group but not in the DCB group. Gene expression analysis of CD14+ leucocytes showed IL18 had decreased expression at two weeks, CXCR4 and IL1\u03b2 decreased at two months, while pentraxin 3 increased at two weeks and two months. In terms of humoral biomarkers, hsTnI remains elevated up to two weeks post PCI while IL6 and TNF\u03b1 remain elevated till two months post PCI.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Intermediate monocytes increase significantly two months following elective, uncomplicated PCI. They remain significantly elevated in the DES group but not in the DCB group suggesting that the PCI strategy could be one of the ways to modulate the inflammatory response post PCI.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0294746","type":"journal-article","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T18:34:42Z","timestamp":1702578882000},"page":"e0294746","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Circulating intermediate monocytes CD14++CD16+ are increased after elective percutaneous coronary intervention"],"prefix":"10.1371","volume":"18","author":[{"given":"Ioannis","family":"Merinopoulos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U","family":"Bhalraam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terri","family":"Holmes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasiliki","family":"Tsampasian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasha","family":"Corballis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tharusha","family":"Gunawardena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Sawh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7508-4785","authenticated-orcid":true,"given":"Clint","family":"Maart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor","family":"Wistow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alisdair","family":"Ryding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon C.","family":"Eccleshall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4005-7752","authenticated-orcid":true,"given":"Vassilios S.","family":"Vassiliou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"key":"pone.0294746.ref001","doi-asserted-by":"crossref","first-page":"3481","DOI":"10.1161\/CIRCULATIONAHA.105.537878","article-title":"27. Pathophysiology of coronary artery disease","volume":"111","author":"P Libby","year":"2005","journal-title":"Circulation"},{"key":"pone.0294746.ref002","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1161\/01.ATV.0000037100.44766.5B","article-title":"Inflammation and restenosis in the stent era","volume":"22","author":"FGP Welt","year":"2002","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"pone.0294746.ref003","article-title":"The role of inflammation in percutaneous coronary intervention, from balloon angioplasty to drug eluting stents","author":"I Merinopoulos","year":"2022","journal-title":"Minerva Cardiol Angiol"},{"key":"pone.0294746.ref004","first-page":"10","article-title":"Inflammation during percutaneous coronary intervention\u2014prognostic value, mechanisms and therapeutic targets","author":"B Tucker","year":"2021","journal-title":"Cells"},{"key":"pone.0294746.ref005","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1016\/j.jacc.2018.12.083","article-title":"Targeting the Immune System in Atherosclerosis: JACC State-of-the-Art Review","volume":"73","author":"TX Zhao","year":"2019","journal-title":"J Am Coll Cardiol"},{"key":"pone.0294746.ref006","doi-asserted-by":"crossref","first-page":"1838","DOI":"10.1056\/NEJMoa2021372","article-title":"Colchicine in Patients with Chronic Coronary Disease","volume":"383","author":"SM Nidorf","year":"2020","journal-title":"N Engl J Med"},{"key":"pone.0294746.ref007","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1016\/j.jacc.2013.07.043","article-title":"Monocytes in coronary artery disease and atherosclerosis: Where are we now?","volume":"62","author":"A Ghattas","year":"2013","journal-title":"J Am Coll Cardiol"},{"key":"pone.0294746.ref008","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.jacc.2003.08.026","article-title":"Circulating Monocytes and In-Stent Neointima after Coronary Stent Implantation","volume":"43","author":"D Fukuda","year":"2004","journal-title":"J Am Coll Cardiol"},{"key":"pone.0294746.ref009","doi-asserted-by":"crossref","first-page":"e16","DOI":"10.1182\/blood-2010-12-326355","article-title":"Gene expression profiling reveals the defining features of the classical, intermediate,and nonclassical human monocyte subsets","volume":"118","author":"KL Wong","year":"2011","journal-title":"Blood"},{"key":"pone.0294746.ref010","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1016\/j.jacc.2012.07.019","article-title":"CD14++CD16+ monocytes independently predict cardiovascular events: A cohort study of 951 patients referred for elective coronary angiography","volume":"60","author":"KS Rogacev","year":"2012","journal-title":"J Am Coll Cardiol"},{"key":"pone.0294746.ref011","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1016\/j.jacc.2010.06.045","article-title":"The evolving role of inflammatory biomarkers in risk assessment after stent implantation","volume":"56","author":"G Niccoli","year":"2010","journal-title":"J Am Coll Cardiol"},{"key":"pone.0294746.ref012","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1016\/S0002-9149(00)01159-0","article-title":"Comparison of activation process of platelets and neutrophils after coronary stent implantation versus balloon angioplasty for stable angina pectoris","volume":"86","author":"T Inoue","year":"2000","journal-title":"Am J Cardiol"},{"key":"pone.0294746.ref013","doi-asserted-by":"crossref","first-page":"45","DOI":"10.2174\/1573403X14666171226144120","article-title":"Percutaneous Coronary Intervention in the Elderly: Are Drug-coated Balloons the Future?","volume":"14","author":"I Merinopoulos","year":"2018","journal-title":"Curr Cardiol Rev"},{"key":"pone.0294746.ref014","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1016\/j.jcin.2020.02.043","article-title":"Drug-Coated Balloons for Coronary Artery Disease: Third Report of the International DCB Consensus Group","volume":"13","author":"R V. 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