{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T18:48:13Z","timestamp":1772218093288,"version":"3.50.1"},"reference-count":83,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T00:00:00Z","timestamp":1717718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01 DC014428"],"award-info":[{"award-number":["R01 DC014428"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01 DC014428"],"award-info":[{"award-number":["R01 DC014428"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["ECR R21 1R21DC017799"],"award-info":[{"award-number":["ECR R21 1R21DC017799"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016221","name":"Rowan University","doi-asserted-by":"publisher","award":["seed fund"],"award-info":[{"award-number":["seed fund"]}],"id":[{"id":"10.13039\/100016221","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016221","name":"Rowan University","doi-asserted-by":"publisher","award":["Summer Undergraduate Research Experience"],"award-info":[{"award-number":["Summer Undergraduate Research Experience"]}],"id":[{"id":"10.13039\/100016221","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016221","name":"Rowan University","doi-asserted-by":"publisher","award":["Summer Medical Research Fellowship"],"award-info":[{"award-number":["Summer Medical Research Fellowship"]}],"id":[{"id":"10.13039\/100016221","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    The Hedgehog (HH) pathway regulates embryonic development of anterior tongue taste fungiform papilla (FP) and the posterior circumvallate (CVP) and foliate (FOP) taste papillae. HH signaling also mediates taste organ maintenance and regeneration in adults. However, there are knowledge gaps in HH pathway component expression during postnatal taste organ differentiation and maturation. Importantly, the HH transcriptional effectors GLI1, GLI2 and GLI3 have not been investigated in early postnatal stages; the HH receptors PTCH1, GAS1, CDON and HHIP, required to either drive HH pathway activation or antagonism, also remain unexplored. Using\n                    <jats:italic>lacZ<\/jats:italic>\n                    reporter mouse models, we mapped expression of the HH ligand SHH, HH receptors, and GLI transcription factors in FP, CVP and FOP in early and late postnatal and adult stages. In adults we also studied the soft palate, and the geniculate and trigeminal ganglia, which extend afferent fibers to the anterior tongue.\n                    <jats:italic>Shh<\/jats:italic>\n                    and\n                    <jats:italic>Gas1<\/jats:italic>\n                    are the only components that were consistently expressed within taste buds of all three papillae and the soft palate. In the first postnatal week, we observed broad expression of HH signaling components in FP and adjacent, non-taste filiform (FILIF) papillae in epithelium or stroma and tongue muscles. Notably, we observed elimination of\n                    <jats:italic>Gli1<\/jats:italic>\n                    in FILIF and\n                    <jats:italic>Gas1<\/jats:italic>\n                    in muscles, and downregulation of\n                    <jats:italic>Ptch1<\/jats:italic>\n                    in lingual epithelium and of\n                    <jats:italic>Cdon<\/jats:italic>\n                    ,\n                    <jats:italic>Gas1<\/jats:italic>\n                    and\n                    <jats:italic>Hhip<\/jats:italic>\n                    in stroma from late postnatal stages. Further, HH receptor expression patterns in CVP and FOP epithelium differed from anterior FP. Among all the components, only known positive regulators of HH signaling, SHH,\n                    <jats:italic>Ptch1<\/jats:italic>\n                    ,\n                    <jats:italic>Gli1<\/jats:italic>\n                    and\n                    <jats:italic>Gli2<\/jats:italic>\n                    , were expressed in the ganglia. Our studies emphasize differential regulation of HH signaling in distinct postnatal developmental periods and in anterior versus posterior taste organs, and lay the foundation for functional studies to understand the roles of numerous HH signaling components in postnatal tongue development.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0294835","type":"journal-article","created":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T14:04:58Z","timestamp":1717769098000},"page":"e0294835","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Distinct expression patterns of Hedgehog signaling components in mouse gustatory system during postnatal tongue development and adult homeostasis"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5675-1389","authenticated-orcid":true,"given":"Archana","family":"Kumari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole E.","family":"Franks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Libo","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabrielle","family":"Audu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Liskowicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte M.","family":"Mistretta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin L.","family":"Allen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,6,7]]},"reference":[{"issue":"2","key":"pone.0294835.ref001","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1002\/ar.1092180217","article-title":"Postnatal development of the vallate papilla and taste buds in rats","volume":"218","author":"MA Hosley","year":"1987","journal-title":"Anat Rec"},{"issue":"3","key":"pone.0294835.ref002","first-page":"123","article-title":"Proliferation of taste buds in the foliate and vallate papillae of postnatal hamsters","volume":"52","author":"IJ Miller","year":"1988","journal-title":"Growth Dev Aging"},{"key":"pone.0294835.ref003","volume-title":"Smell and taste in health and disease","author":"Raven Press","year":"1991"},{"issue":"1","key":"pone.0294835.ref004","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0165-3806(83)90205-5","article-title":"Developmental changes in taste responses from glossopharyngeal nerve in sheep and comparisons with chorda tympani responses","volume":"313","author":"CM Mistretta","year":"1983","journal-title":"Brain Res"},{"issue":"2","key":"pone.0294835.ref005","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/(SICI)1096-9861(19990405)406:2<143::AID-CNE1>3.0.CO;2-X","article-title":"Expression of sonic hedgehog, patched, and Gli1 in developing taste papillae of the mouse","volume":"406","author":"JM Hall","year":"1999","journal-title":"J Comp Neurol"},{"issue":"1","key":"pone.0294835.ref006","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/(SICI)1096-9861(19990621)409:1<13::AID-CNE2>3.0.CO;2-O","article-title":"Alterations in size, number, and morphology of gustatory papillae and taste buds in BDNF null mutant mice demonstrate neural dependence of developing taste organs","volume":"409","author":"CM Mistretta","year":"1999","journal-title":"J Comp Neurol"},{"issue":"4","key":"pone.0294835.ref007","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1679\/aohc.69.199","article-title":"Development of fungiform papillae: patterned lingual gustatory organs","volume":"69","author":"CM Mistretta","year":"2006","journal-title":"Arch Histol Cytol"},{"key":"pone.0294835.ref008","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.64013","article-title":"Onset of taste bud cell renewal starts at birth and coincides with a shift in SHH function","volume":"10","author":"EJ Golden","year":"2021","journal-title":"Elife"},{"issue":"1","key":"pone.0294835.ref009","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.ydbio.2013.07.022","article-title":"Multiple Shh Signaling Centers Participate in Fungiform Papilla and Taste Bud Formation and Maintenance","volume":"382","author":"HX Liu","year":"2013","journal-title":"Dev Biol"},{"issue":"6","key":"pone.0294835.ref010","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.archoralbio.2008.01.005","article-title":"Quantitative study of taste bud distribution within the oral cavity of the postnatal mouse","volume":"53","author":"GH Zhang","year":"2008","journal-title":"Arch Oral Biol"},{"issue":"1","key":"pone.0294835.ref011","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/j.1748-1716.1970.tb04704.x","article-title":"Reformation of taste buds by crossed sensory nerves in the rat\u2019s tongue","volume":"79","author":"B. Oakley","year":"1970","journal-title":"Acta Physiol Scand"},{"issue":"2","key":"pone.0294835.ref012","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0165-3806(91)90007-6","article-title":"The rate and locus of development of rat vallate taste buds","volume":"58","author":"B Oakley","year":"1991","journal-title":"Brain Res Dev Brain Res"},{"key":"pone.0294835.ref013","first-page":"109","article-title":"The primate gustatory system","author":"TC Pritchard","year":"1991","journal-title":"Smell and taste in health and disease"},{"issue":"11","key":"pone.0294835.ref014","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1007\/s00702-010-0480-x","article-title":"Facilitation of the development of fungiform taste buds by early intraoral acesulfame-K stimulation to mice","volume":"117","author":"GH Zhang","year":"2010","journal-title":"J Neural Transm (Vienna)"},{"issue":"6","key":"pone.0294835.ref015","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1007\/s00429-006-0112-2","article-title":"Developmental change of alpha-gustducin expression in the mouse fungiform papilla","volume":"211","author":"GH Zhang","year":"2006","journal-title":"Anat Embryol (Berl)"},{"issue":"3","key":"pone.0294835.ref016","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/S0031-9384(99)00184-5","article-title":"Maturation of taste buds on the soft palate of the postnatal rat","volume":"68","author":"S Harada","year":"2000","journal-title":"Physiol Behav"},{"issue":"2","key":"pone.0294835.ref017","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ydbio.2015.07.006","article-title":"Postnatal reduction of BDNF regulates the developmental remodeling of taste bud innervation","volume":"405","author":"T Huang","year":"2015","journal-title":"Dev 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Signaling in Adult Organ Homeostasis and Repair","volume":"141","author":"R Petrova","year":"2014","journal-title":"Development"},{"issue":"11","key":"pone.0294835.ref025","doi-asserted-by":"crossref","first-page":"e1006442","DOI":"10.1371\/journal.pgen.1006442","article-title":"Maintenance of Taste Organs is Strictly Dependent on Epithelial Hedgehog\/GLI Signaling","volume":"12","author":"AN Ermilov","year":"2016","journal-title":"PLoS Genet"},{"issue":"48","key":"pone.0294835.ref026","doi-asserted-by":"crossref","first-page":"E10369","DOI":"10.1073\/pnas.1712881114","article-title":"Recovery of Taste Organs and Sensory Function after Severe Loss from Hedgehog\/Smoothened Inhibition with Cancer Drug Sonidegib","volume":"114","author":"A Kumari","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"pone.0294835.ref027","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1002\/dvdy.456","article-title":"Unique lingual expression of the Hedgehog pathway antagonist Hedgehog-interacting protein in filiform papillae during homeostasis and ectopic expression in fungiform papillae during Hedgehog signaling inhibition","volume":"251","author":"A Kumari","year":"2022","journal-title":"Dev Dyn"},{"issue":"1","key":"pone.0294835.ref028","doi-asserted-by":"crossref","first-page":"16150","DOI":"10.1038\/s41598-018-34399-3","article-title":"Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib","volume":"8","author":"A Kumari","year":"2018","journal-title":"Sci Rep"},{"issue":"2","key":"pone.0294835.ref029","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.ydbio.2004.07.042","article-title":"Sonic hedgehog exerts distinct, stage-specific effects on tongue and taste papilla development","volume":"276","author":"HX Liu","year":"2004","journal-title":"Dev Biol"},{"issue":"1","key":"pone.0294835.ref030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0012-1606(02)00014-3","article-title":"Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium","volume":"254","author":"CM Mistretta","year":"2003","journal-title":"Dev Biol"},{"issue":"7","key":"pone.0294835.ref031","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1038\/nrm3598","article-title":"The Mechanisms of Hedgehog Signalling and Its Roles in Development and Disease","volume":"14","author":"J Briscoe","year":"2013","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"6605","key":"pone.0294835.ref032","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1038\/384129a0","article-title":"The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog","volume":"384","author":"DM Stone","year":"1996","journal-title":"Nature"},{"issue":"6900","key":"pone.0294835.ref033","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1038\/nature00989","article-title":"Patched acts catalytically to suppress the activity of Smoothened","volume":"418","author":"J Taipale","year":"2002","journal-title":"Nature"},{"issue":"6","key":"pone.0294835.ref034","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.devcel.2011.04.018","article-title":"Overlapping roles and collective requirement for the coreceptors GAS1, CDO, and BOC in SHH pathway function","volume":"20","author":"BL Allen","year":"2011","journal-title":"Dev Cell"},{"issue":"16","key":"pone.0294835.ref035","doi-asserted-by":"crossref","first-page":"3423","DOI":"10.1242\/dev.095083","article-title":"Essential role for ligand-dependent feedback antagonism of vertebrate hedgehog signaling by PTCH1, PTCH2 and HHIP1 during neural patterning","volume":"140","author":"AM Holtz","year":"2013","journal-title":"Development"},{"issue":"1","key":"pone.0294835.ref036","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1242\/dev.01566","article-title":"Growth and pattern of the mammalian neural tube are governed by partially overlapping feedback activities of the hedgehog antagonists patched 1 and Hhip1","volume":"132","author":"J Jeong","year":"2005","journal-title":"Development"},{"key":"pone.0294835.ref037","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1146\/annurev-cellbio-092910-154048","article-title":"Gli proteins in development and disease","volume":"27","author":"CC Hui","year":"2011","journal-title":"Annu Rev Cell Dev Biol"},{"issue":"2","key":"pone.0294835.ref038","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.neuron.2012.05.018","article-title":"Sonic hedgehog maintains cellular and neurochemical homeostasis in the adult nigrostriatal circuit","volume":"75","author":"LE Gonzalez-Reyes","year":"2012","journal-title":"Neuron"},{"issue":"6","key":"pone.0294835.ref039","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/S0896-6273(03)00561-0","article-title":"Sonic hedgehog is required for progenitor cell maintenance in telencephalic stem cell niches","volume":"39","author":"R Machold","year":"2003","journal-title":"Neuron"},{"issue":"5329","key":"pone.0294835.ref040","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1126\/science.277.5329.1109","article-title":"Altered neural cell fates and medulloblastoma in mouse patched mutants","volume":"277","author":"LV Goodrich","year":"1997","journal-title":"Science"},{"issue":"5","key":"pone.0294835.ref041","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S0960-9822(03)00088-5","article-title":"Microform holoprosencephaly in mice that lack the Ig superfamily member Cdon","volume":"13","author":"F Cole","year":"2003","journal-title":"Curr Biol"},{"issue":"3","key":"pone.0294835.ref042","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1101\/gad.1026303","article-title":"Feedback control of mammalian Hedgehog signaling by the Hedgehog-binding protein, Hip1, modulates Fgf signaling during branching morphogenesis of the lung","volume":"17","author":"PT Chuang","year":"2003","journal-title":"Genes Dev"},{"issue":"24","key":"pone.0294835.ref043","doi-asserted-by":"crossref","first-page":"5161","DOI":"10.1242\/dev.128.24.5161","article-title":"Gli1 can rescue the in vivo function of Gli2","volume":"128","author":"CB Bai","year":"2001","journal-title":"Development"},{"issue":"41","key":"pone.0294835.ref044","doi-asserted-by":"crossref","first-page":"13597","DOI":"10.1523\/JNEUROSCI.0830-10.2010","article-title":"Sonic hedgehog regulates discrete populations of astrocytes in the adult mouse forebrain","volume":"30","author":"AD Garcia","year":"2010","journal-title":"J Neurosci"},{"issue":"17","key":"pone.0294835.ref045","first-page":"3054","article-title":"Sonic hedgehog from both nerves and epithelium is a key trophic factor for taste bud maintenance","volume":"144","author":"D Castillo-Azofeifa","year":"2017","journal-title":"Development"},{"issue":"2","key":"pone.0294835.ref046","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s00441-021-03561-1","article-title":"Probing the multimodal fungiform papilla: complex peripheral nerve endings of chorda tympani taste and mechanosensitive fibers before and after Hedgehog pathway inhibition","volume":"387","author":"CR Donnelly","year":"2021","journal-title":"Cell Tissue Res"},{"issue":"2","key":"pone.0294835.ref047","first-page":"E200","article-title":"Neuronal delivery of Hedgehog directs spatial patterning of taste organ regeneration","volume":"115","author":"W-J Lu","year":"2018","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"pone.0294835.ref048","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1152\/jn.00822.2014","article-title":"Hedgehog Pathway Blockade with the Cancer Drug LDE225 Disrupts Taste Organs and Taste Sensation","volume":"113","author":"A Kumari","year":"2015","journal-title":"J Neurophysiol"},{"issue":"2","key":"pone.0294835.ref049","doi-asserted-by":"crossref","first-page":"e1007058","DOI":"10.1371\/journal.pgen.1007058","article-title":"Gli3 is a Negative Regulator of Tas1r3-Expressing Taste Cells","volume":"14","author":"Y Qin","year":"2018","journal-title":"PLoS Genet"},{"issue":"8","key":"pone.0294835.ref050","doi-asserted-by":"crossref","first-page":"4593","DOI":"10.1128\/jvi.69.8.4593-4599.1995","article-title":"Long-term expression in sensory neurons in tissue culture from herpes simplex virus type 1 (HSV-1) promoters in an HSV-1-derived vector","volume":"69","author":"RL Smith","year":"1995","journal-title":"J Virol"},{"issue":"2","key":"pone.0294835.ref051","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1002\/cne.21738","article-title":"Expression of the basal cell markers of taste buds in the anterior tongue and soft palate of the mouse embryo","volume":"509","author":"A Nakayama","year":"2008","journal-title":"J Comp Neurol"},{"issue":"5","key":"pone.0294835.ref052","doi-asserted-by":"crossref","DOI":"10.3390\/ijms24054833","article-title":"Anterior and Posterior Tongue Regions and Taste Papillae: Distinct Roles and Regulatory Mechanisms with an Emphasis on Hedgehog Signaling and Antagonism","volume":"24","author":"A Kumari","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"4","key":"pone.0294835.ref053","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1679\/aohc.69.209","article-title":"Cell lineage and differentiation in taste buds","volume":"69","author":"H Miura","year":"2006","journal-title":"Arch Histol 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