{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:02:17Z","timestamp":1787022137595,"version":"build-2736575974"},"reference-count":56,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["20K194130"],"award-info":[{"award-number":["20K194130"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>This systematic review and meta-analysis examined the efficacy of neuromuscular electrical stimulation (NMES) on lower limb muscle strength and health-related quality of life (HR-QOL) after thoracic and abdominal surgery. We searched the Cochrane Central Register of Controlled Trials, MEDLINE via PubMed, Excerpta Medica Database via Elsevier, Physiotherapy Evidence Database, Cumulative Index to Nursing and Allied Health Literature, World Health Organization International Clinical Trials Registry Platform via their dedicated search portal, and ClinicalTrials.gov on November 2021 and updated in April 2023 to identify randomized controlled trials that examined the effects of NMES after thoracic and abdominal surgery. The primary outcomes were lower limb muscle strength, HR-QOL, and adverse events. We used the Cochrane Risk of Bias Tool and the Grading of Recommendations, Assessment, Development, and Evaluation approach to assess the certainty of evidence. A total of 18 randomized control trials involving 915 participants, including 10 on cardiovascular surgery, two on pulmonary surgery, five on digestive system surgery, and one on other surgery, were included. NMES slightly increased lower limb muscle strength and adverse events in cardiovascular surgery. Adverse events (hypotension, pain, and muscle discomfort) occurred in seven patients. HR-QOL was measured in two studies on cardiovascular surgery, but these were not pooled due to concept heterogeneity. Overall, NMES slightly increases lower limb muscle strength after cardiovascular surgery without serious adverse events. However, higher-quality randomized control trials in thoracic and abdominal surgeries are needed.<\/jats:p>","DOI":"10.1371\/journal.pone.0294965","type":"journal-article","created":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T13:29:53Z","timestamp":1701350993000},"page":"e0294965","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Efficacy of neuromuscular electrical stimulation for thoracic and abdominal surgery: A systematic review and meta-analysis"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7838-9472","authenticated-orcid":true,"given":"Yuki","family":"Nakashima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Iwaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Kataoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Ariie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsuke","family":"Taito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0088-8447","authenticated-orcid":true,"given":"Yuichi","family":"Nishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Mio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukio","family":"Mikami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,11,30]]},"reference":[{"key":"pone.0294965.ref001","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/j.jamcollsurg.2004.05.280","article-title":"Functional independence after major abdominal surgery in the elderly","volume":"199","author":"VA Lawrence","year":"2004","journal-title":"J Am Coll Surg"},{"key":"pone.0294965.ref002","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1093\/icvts\/ivs403","article-title":"Does the mode of exercise influence recovery of functional capacity in the early postoperative period after coronary artery bypass graft surgery? 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Introduction\u2014GRADE evidence profiles and summary of findings tables","volume":"64","author":"G Guyatt","year":"2011","journal-title":"J Clin Epidemiol"},{"key":"pone.0294965.ref029","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.jclinepi.2019.10.014","article-title":"GRADE guidelines 26: informative statements to communicate the findings of systematic reviews of interventions","volume":"119","author":"N Santesso","year":"2020","journal-title":"J Clin Epidemiol"},{"key":"pone.0294965.ref030","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s13054-016-1199-3","article-title":"Muscle mass, strength and functional outcomes in critically ill patients after cardiothoracic surgery: does neuromuscular electrical stimulation help? 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Effects a Rehabilitation Program in Patients With Left Ventricular Dysfunction After Coronary Artery Bypass Grafting. 2016 [Cited 16 June 2023]. In: ClinicalTrials.gov [Internet]. Available from: https:\/\/clinicaltrials.gov\/ct2\/show\/NCT02763774.ClinicalTrials.gov."},{"key":"pone.0294965.ref034","doi-asserted-by":"crossref","first-page":"e13012","DOI":"10.1097\/MD.0000000000013012","article-title":"Ambulation capacity and functional outcome in patients undergoing neuromuscular electrical stimulation after cardiac valve surgery A randomised clinical trial","volume":"97","author":"TC Fontes Cerqueira","year":"2018","journal-title":"Medicine (Baltimore)"},{"key":"pone.0294965.ref035","doi-asserted-by":"crossref","first-page":"e22769","DOI":"10.1097\/MD.0000000000022769","article-title":"Neuromuscular electrical stimulation in early rehabilitation of patients with postoperative complications after cardiovascular surgery: A randomized controlled trial","volume":"99","author":"AN Sumin","year":"2020","journal-title":"Medicine (Baltimore)"},{"key":"pone.0294965.ref036","unstructured":"Kentaro Kiryu. The effectiveness of electrical muscle stimulation in patients after open heart surgery. 2020 [Cited 16 June 2023]. In: UMIN-CTR Clinical Trial [Internet]. Available from: https:\/\/center6.umin.ac.jp\/cgi-open-bin\/ctr_e\/ctr_view.cgi?recptno=R000045532. UMIN-CTR Clinical Trial. 2020."},{"key":"pone.0294965.ref037","doi-asserted-by":"crossref","first-page":"101659","DOI":"10.1016\/j.rehab.2022.101659","article-title":"Neuromuscular electrical stimulation after cardiovascular surgery mitigates muscle weakness in older individuals with diabetes","volume":"66","author":"K Takino","year":"2023","journal-title":"Ann Phys Rehabil Med"},{"key":"pone.0294965.ref038","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1177\/02692155211070945","article-title":"Effect of neuromuscular electrical stimulation on functional exercise capacity in patients undergoing cardiac surgery: A randomized clinical trial","volume":"36","author":"TCF Cerqueira","year":"2022","journal-title":"Clin Rehabil"},{"key":"pone.0294965.ref039","doi-asserted-by":"crossref","first-page":"32","DOI":"10.21653\/tjpr.951439","article-title":"Short Term Effects of Cardiopulmonary Rehabilitation and Neuromuscular Electrical Stimulation on Functional Capacity, Myocardial Tissue Doppler after Coronary Artery Bypass Graft Surgery","volume":"33","author":"B \u00d6z\u00fcberk","year":"2022","journal-title":"T\u00fcrk Fizyoterapi ve Rehabilitasyon Dergisi"},{"key":"pone.0294965.ref040","doi-asserted-by":"crossref","first-page":"S359","DOI":"10.1016\/j.healun.2021.01.1012","article-title":"Nutritional Supplementation and Neuromuscular Electrical Stimulation in Lung Transplant Patients","volume":"40","author":"I Timofte","year":"2021","journal-title":"The Journal of Heart and Lung Transplantation"},{"key":"pone.0294965.ref041","unstructured":"Zaragoza-Garc\u00eda. Electrostimulation Program for Lung Transplant Recipients in the ICU. 2022 [cited 2023 May 23]. In: ClinicalTrials.gov [Internet]. Available from: https:\/\/ClinicalTrials.gov\/show\/NCT05410522"},{"key":"pone.0294965.ref042","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1038\/s41366-021-00812-7","article-title":"Whole-body electrical stimulation as a strategy to improve functional capacity and preserver lean mass after bariatric surgery: a randomized triple-blind controlled trial","volume":"45","author":"LD Andr\u00e9","year":"2021","journal-title":"Int J Obes (Lond)"},{"key":"pone.0294965.ref043","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1177\/0269215518821718","article-title":"Effects of quadriceps muscle neuromuscular electrical stimulation in living donor liver transplant recipients: phase-II single-blinded randomized controlled trial","volume":"33","author":"M Hanada","year":"2019","journal-title":"Clin Rehabil"},{"key":"pone.0294965.ref044","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1186\/s13063-021-05573-2","article-title":"BiCyCLE NMES\u2014neuromuscular 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