{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T05:56:05Z","timestamp":1778219765545,"version":"3.51.4"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T00:00:00Z","timestamp":1701734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Senju Pharmaceutical Co., Ltd."}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Purpose<\/jats:title>\n<jats:p>The purpose of this study was to investigate the effect of a 1% brinzolamide and 0.1% brimonidine fixed combination (BBFC) on ONH blood flow (BF) in rabbits.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>A crossover study was conducted on pigmented rabbits; a physiological saline solution, brinzolamide, or BBFC was administered for eight days. ONH BF, intraocular pressure (IOP) and systemic parameters were measured before the eighth day\u2019s first dose and at 6, 9, 12, and 14 hours after the dose. ONH BF was assessed using laser speckle flowgraphy, and mean blur rate (MBR) values were calculated. The percentage against baseline of each parameter was calculated, and intergroup comparisons were performed at each time point.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>There were no significant differences in the percentage change in systemic parameters. At 6 hours after administration, the BBFC group showed a significantly higher percentage change in large vessel area-MBR (%MV) compared to the control group (98.6\u00b116.8%MV vs. 81.3\u00b17.9%MV, P = 0.03). On the other hand, the brinzolamide group did not show a significant difference. Both the brinzolamide and BBFC groups had significantly lower percentage change in IOP (%IOP) compared to the control group (90.6\u00b15.0%IOP, 93.3\u00b12.9%IOP, and 99.2\u00b11.7%IOP, respectively, P &lt; 0.01).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>BBFC effectively reduces IOP and mitigates diurnal fluctuation-induced decreases in ONH BF.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0295122","type":"journal-article","created":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T18:28:40Z","timestamp":1701800920000},"page":"e0295122","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["The effect of a brinzolamide\/brimonidine fixed combination on optic nerve head blood flow in rabbits"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2816-1338","authenticated-orcid":true,"given":"Nana","family":"Takahashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kota","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Kiyota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eriko","family":"Kunikane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5591-4155","authenticated-orcid":true,"given":"Toru","family":"Nakazawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,12,5]]},"reference":[{"issue":"4","key":"pone.0295122.ref001","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1080\/09286580701501931","article-title":"Open-angle glaucoma\u2014an epidemiologic overview.","volume":"14","author":"MC Leske","year":"2007","journal-title":"Ophthalmic Epidemiol"},{"issue":"10108","key":"pone.0295122.ref002","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1016\/S0140-6736(17)31469-1","article-title":"Glaucoma.","volume":"390","author":"JB Jonas","year":"2017","journal-title":"Lancet"},{"issue":"4","key":"pone.0295122.ref003","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1136\/bjophthalmol-2013-304323","article-title":"Variation of clinical outcomes used in glaucoma randomised controlled trials: a systematic review","volume":"98","author":"R Ismail","year":"2014","journal-title":"Br J Ophthalmol"},{"issue":"4","key":"pone.0295122.ref004","first-page":"487","article-title":"Collaborative Normal-Tension Glaucoma Study Group.","volume":"126","author":"Comparison of glaucomatous progression between untreated patients with normal-tension glaucoma and patients with therapeutically reduced intraocular pressures.","year":"1998","journal-title":"Am J Ophthalmol"},{"issue":"4","key":"pone.0295122.ref005","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/S0002-9394(00)00538-9","article-title":"The relationship between control of intraocular pressure and visual field deterioration.The AGIS Investigators","volume":"130","author":"The Advanced Glaucoma Intervention Study (AGIS): 7","year":"2000","journal-title":"Am J Ophthalmol"},{"issue":"3","key":"pone.0295122.ref006","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1097\/00061198-199906000-00012","article-title":"Vascular dysregulation: a principal risk factor for glaucomatous damage","volume":"8","author":"J Flammer","year":"1999","journal-title":"J Glaucoma"},{"issue":"1","key":"pone.0295122.ref007","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1177\/112067219400400105","article-title":"Influence of nifedipine on the visual fields of patients with optic-nerve-head diseases.","volume":"4","author":"AZ Gaspar","year":"1994","journal-title":"Eur J Ophthalmol"},{"issue":"1","key":"pone.0295122.ref008","doi-asserted-by":"crossref","first-page":"S27","DOI":"10.1016\/S0039-6257(99)00018-1","article-title":"The role of age and cardiovascular disease in glaucomatous optic neuropathy.","volume":"43","author":"SS Hayreh","year":"1999","journal-title":"Surv Ophthalmol."},{"issue":"1","key":"pone.0295122.ref009","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1097\/APO.0000000000000183","article-title":"Ocular Blood Flow and Influencing Factors for Glaucoma","volume":"5","author":"T. 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