{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T20:38:34Z","timestamp":1781642314901,"version":"3.54.5"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K00890"],"award-info":[{"award-number":["16K00890"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K019760005"],"award-info":[{"award-number":["23K019760005"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23580173"],"award-info":[{"award-number":["23580173"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Hepatic steatosis is an early stage in the progression of non-alcoholic fatty liver disease (NAFLD) and can lead to the development of non-alcoholic steatohepatitis (NASH), a major cause of liver-related morbidity and mortality. Identification of dietary components that can alleviate hepatic steatosis is crucial for developing effective therapeutic strategies for NAFLD. Recently, we demonstrated the impact of lipids extracted from the marine red alga Susabinori (<jats:italic>Pyropia yezoensis<\/jats:italic>) in a murine model of type 2-diabete (<jats:italic>db<\/jats:italic>\/<jats:italic>db<\/jats:italic>). We found that Susabinori lipids (SNL), abundant in eicosapentaenoic acid (EPA)-containing polar lipids, protected against obesity-induced hepatic steatosis in <jats:italic>db<\/jats:italic>\/<jats:italic>db<\/jats:italic> mice. To understand the specific genes or biological pathways underlying the effects of SNL, we conducted RNA-Seq analysis of the hepatic transcriptome. By performing comparative analysis of differentially expressed genes between normal mice and <jats:italic>db<\/jats:italic>\/<jats:italic>db<\/jats:italic> mice consuming a control diet, as well as SNL-fed <jats:italic>db<\/jats:italic>\/<jats:italic>db<\/jats:italic> mice, we identified the 15 SNL-dependent up-regulated genes that were down-regulated in <jats:italic>db<\/jats:italic>\/<jats:italic>db<\/jats:italic> mice but up-regulated by SNL feeding. Gene ontology and pathway analysis on these 15 genes demonstrated a significant association with the metabolisms of arachidonic acid (AA) and linoleic acid (LA). Furthermore, we observed alterations in the expression levels of monoacylglycerol lipase (<jats:italic>Magl<\/jats:italic>) and fatty acid-binding protein 4 (<jats:italic>Fabp4<\/jats:italic>) in the SNL-fed <jats:italic>db<\/jats:italic>\/<jats:italic>db<\/jats:italic> mice, both of which are implicated in AA and LA metabolism. Additionally, the livers of SNL-fed <jats:italic>db<\/jats:italic>\/<jats:italic>db<\/jats:italic> mice exhibited reduced levels of AA and LA, but a high accumulation of EPA. In conclusion, the SNL diet might affect the metabolisms of AA and LA, which contribute to the improvement of hepatic steatosis. Our findings provide insights into the molecular mechanisms underlying the beneficial effects of SNL.<\/jats:p>","DOI":"10.1371\/journal.pone.0295591","type":"journal-article","created":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T18:22:44Z","timestamp":1702405364000},"page":"e0295591","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification of genes regulated by lipids from seaweed Susabinori (Pyropia yezoensis) involved in the improvement of hepatic steatosis: Insights from RNA-Seq analysis in obese db\/db mice"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0282-0160","authenticated-orcid":true,"given":"Sayaka","family":"Iizasa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Nagao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Tsuge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5030-676X","authenticated-orcid":true,"given":"Yukio","family":"Nagano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruyoshi","family":"Yanagita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,12,12]]},"reference":[{"issue":"3","key":"pone.0295591.ref001","doi-asserted-by":"crossref","first-page":"97","DOI":"10.3109\/10408363.2011.596521","article-title":"Non-alcoholic fatty liver disease","volume":"48","author":"BW Smith","year":"2011","journal-title":"Crit Rev Clin Lab Sci"},{"key":"pone.0295591.ref002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12902-022-00980-1","article-title":"Non-alcoholic fatty liver disease (NAFLD): a review of pathophysiology, clinical management and effects of weight loss","volume":"22","author":"S Pouwels","year":"2022","journal-title":"BMC Endocr Disord"},{"key":"pone.0295591.ref003","first-page":"17","article-title":"Diabetes mellitus, obesity, and hepatic steatosis","volume":"13","author":"W Youssef","year":"2002","journal-title":"Semin Gastrointest Dis"},{"issue":"10290","key":"pone.0295591.ref004","doi-asserted-by":"crossref","first-page":"2212","DOI":"10.1016\/S0140-6736(20)32511-3","article-title":"Non-alcoholic fatty liver disease","volume":"397","author":"EE Powell","year":"2021","journal-title":"Lancet Lond Engl"},{"issue":"4","key":"pone.0295591.ref005","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1111\/jpy.12992","article-title":"Redefining Pyropia (Bangiales, Rhodophyta): Four New Genera, Resurrection of Porphyrella and Description of Calidia pseudolobata sp. nov. 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