{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T23:01:41Z","timestamp":1789599701673,"version":"build-2803163510"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T00:00:00Z","timestamp":1703116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["302080\/2022-8"],"award-info":[{"award-number":["302080\/2022-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Mosquitoes of the genus <jats:italic>Sabethes<\/jats:italic> are exclusively sylvatic species occurring in Central and South Americas, where they play a role in the transmission of arbovirus. S<jats:italic>abethes identicus<\/jats:italic>, a common bamboo-breeder species, has been found naturally infected with yellow fever virus. Our study aimed to describe biological and behavioral features of <jats:italic>Sa<\/jats:italic>. <jats:italic>identicus<\/jats:italic> in the laboratory, as well as establish and standardize an isolated colony for experimental assays. We tested different larval densities and evaluated larval and pupal development time, oviposition behavior, egg production, and longevity. We also shot and video-documented bionomics and behavioral aspects of the mosquitoes in the field and laboratory. A colony with more than 30 generations was achieved. Embryogenesis of <jats:italic>Sa<\/jats:italic>. <jats:italic>identicus<\/jats:italic> is completed in about three days with a 79% hatch rate, while larval and pupal development takes approximately 15\u201317 and nine days, respectively. The mosquito\u2019s entire life cycle lasts approximately 30 days. Adult females could survive 71 days, and each individual laid an average of 88 eggs over their lifetime; 50% of females and males survived 37 and 24 days, respectively. Hematophagy peaks as early as the first week of emergence. The net content of a bamboo internode influenced the choice for oviposition, with the average number of eggs laid in those containing rearing water with larval and pupal exuviae being significantly higher than when they had only dechlorinated water or water with yeast. We documented for the first time an ecological association of weevils and <jats:italic>Sa<\/jats:italic>. <jats:italic>identicus<\/jats:italic>, where the oviposition of the latter depends on the weevil creating a hole in the bamboo wall for egg-laying purposes. Video recordings revealed for the first time the peculiar movements of gravid females during egg-throwing into tiny bamboo holes, as well as the laborious escape of newly emerged adults from the bamboo cavity, and mating behavior.<\/jats:p>","DOI":"10.1371\/journal.pone.0296289","type":"journal-article","created":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T18:54:57Z","timestamp":1703184897000},"page":"e0296289","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Biological and behavioral features and colonization of the sylvatic mosquito Sabethes identicus (Diptera: Culicidae)"],"prefix":"10.1371","volume":"18","author":[{"given":"Maria Ignez","family":"Lima Bersot","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genilton","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Rodrigo","family":"De Moraes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glauber","family":"Rocha Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"Albuquerque Motta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0423-5694","authenticated-orcid":true,"given":"Ricardo","family":"Louren\u00e7o-De-Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,12,21]]},"reference":[{"key":"pone.0296289.ref001","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.jcv.2014.08.030","article-title":"Yellow fever.","volume":"64","author":"TP Monath","year":"2015","journal-title":"J Clin Virol"},{"key":"pone.0296289.ref002","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1093\/jmedent\/27.6.1041","article-title":"Seasonal abundance and diel landing periodicity of Sabethes chloropterus (Diptera: Culicidae) in Trinidad, West Indies.","volume":"27","author":"DD Chadee","year":"1990","journal-title":"J Med. Entomol"},{"issue":"1","key":"pone.0296289.ref003","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1080\/22221751.2019.1568180","article-title":"Haemagogus leucocelaenus and Haemagogus janthinomys are the primary vectors in the major yellow fever outbreak in Brazil, 2016\u20132018.","volume":"8","author":"FVS de Abreu","year":"2019","journal-title":"Emerg Microbes Infect"},{"issue":"3","key":"pone.0296289.ref004","doi-asserted-by":"crossref","first-page":"757","DOI":"10.3390\/v15030757","article-title":"Yellow Fever Virus Maintained by Sabethes mosquitoes during the dry season in Cerrado, a semiarid region of Brazil, in 2021.","volume":"15","author":"CH de Oliveira","year":"2023","journal-title":"Viruses"},{"key":"pone.0296289.ref005","first-page":"409","volume-title":"Instituto Evandro Chagas. 50 anos de contribui\u00e7\u00e3o \u00e0s ci\u00eancias biol\u00f3gicas e \u00e0 medicina tropical.","author":"JP Herv\u00e9","year":"1986"},{"key":"pone.0296289.ref006","first-page":"136","article-title":"New entomological and virological data on the vectors of sylvatic yellow fever in Brazil.","volume":"44","author":"N D\u00e9gallier","year":"1992","journal-title":"Brazil J Assoc Adv Sci"},{"key":"pone.0296289.ref007","first-page":"72","volume-title":"An Overview of arbovirology in Brazil and neighbouring countries","author":"PFC Vasconcelos","year":"1998"},{"key":"pone.0296289.ref008","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1089\/vbz.2011.0730","article-title":"Isolation of yellow fever virus from mosquitoes in Misiones Province, Argentina.","volume":"12","author":"S Goenaga","year":"2012","journal-title":"Vector-Borne and Zoonotic Dis"},{"issue":"12","key":"pone.0296289.ref009","doi-asserted-by":"crossref","first-page":"2805","DOI":"10.3390\/v14122805","article-title":"Back to where it was first described. Vectors of sylvatic yellow fever transmission in the 2017 outbreak in Esp\u00edrito Santo, Brazil","volume":"14","author":"LMA Stanzani","year":"2023","journal-title":"Viruses"},{"key":"pone.0296289.ref010","doi-asserted-by":"crossref","first-page":"98","DOI":"10.4269\/ajtmh.1951.s1-31.98","article-title":"Ecological observations on forest mosquitoes of an endemic yellow fever area of Panama","volume":"31","author":"P Galindo","year":"1951","journal-title":"Am J Trop Med"},{"key":"pone.0296289.ref011","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1093\/aesa\/48.3.158","article-title":"A contribution to the ecology and biology of tree hole breeding mosquitoes of Panama","volume":"48","author":"P Galindo","year":"1955","journal-title":"Ann Entomol Soc Am"},{"issue":"2","key":"pone.0296289.ref012","first-page":"97","article-title":"Comportamento de pouso sobre partes do corpo humano, em mosquitos da Floresta Amaz\u00f4nica.","volume":"6","author":"N D\u00e9gallier","year":"1990","journal-title":"Diptera (Culic\u00eddae). 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