{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:09:39Z","timestamp":1789693779825,"version":"4.0.1"},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T00:00:00Z","timestamp":1705017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"name":"Thailand Science Research and Innovation Fund at Chulalongkorn University","award":["HEA663100108"],"award-info":[{"award-number":["HEA663100108"]}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"crossref","award":["N42A660897"],"award-info":[{"award-number":["N42A660897"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007707","name":"University of California, Davis","doi-asserted-by":"publisher","award":["A22-3754-S001"],"award-info":[{"award-number":["A22-3754-S001"]}],"id":[{"id":"10.13039\/100007707","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Thailand Science Research and Innovation, and Thailand Research Fund","award":["MRG6280029"],"award-info":[{"award-number":["MRG6280029"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>This study investigated the prevalence and antimicrobial resistance (AMR) of <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E. coli<\/jats:italic>) in Nile tilapia from fresh markets and supermarkets. A total of samples (<jats:italic>n<\/jats:italic> = 828) were collected from Nile tilapia including fish flesh (<jats:italic>n<\/jats:italic> = 276), liver and kidney (<jats:italic>n<\/jats:italic> = 276), and intestine (<jats:italic>n<\/jats:italic> = 276). Overall prevalence of fecal coliforms (61.6%) and <jats:italic>E. coli<\/jats:italic> (53.0%) were observed. High prevalence of <jats:italic>E. coli<\/jats:italic> was found in the intestine (71.4%), followed by the liver and kidney (45.7%). The highest prevalence of resistance was commonly found against tetracycline (78.5%), ampicillin (72.8%), and sulfamethoxazole (45.6%) with resistance to only tetracycline (15.2%) as the most common antibiogram. The prevalence of multidrug resistance (MDR) (54.4%) and Extended-spectrum beta-lactamases (ESBLs) (5.7%) were examined. The predominant virulence genes (<jats:italic>n<\/jats:italic> = 158) were <jats:italic>st<\/jats:italic> (14.6%), followed by <jats:italic>eaeA<\/jats:italic> (0.6%). The <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub> (73.4%), <jats:italic>tetA<\/jats:italic> (65.2%), and <jats:italic>qnrS<\/jats:italic> (57.6%). There is statistical significance between Nile tilapia from fresh markets and supermarkets. Based on logistic regression analysis, ampicillin-resistant <jats:italic>E. coli<\/jats:italic> was statistically associated with the phenotypic resistance to tetracycline and trimethoprim, and the presence of <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub> and <jats:italic>tetA<\/jats:italic> (<jats:italic>p<\/jats:italic> &lt; 0.05). Further investigation of AMR transference and their mechanisms is needed for AMR control.<\/jats:p>","DOI":"10.1371\/journal.pone.0296857","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T18:36:50Z","timestamp":1705084610000},"page":"e0296857","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Antimicrobial resistance, virulence profile, and genetic analysis of ESBL-producing Escherichia coli isolated from Nile tilapia in fresh markets and supermarkets in Thailand"],"prefix":"10.1371","volume":"19","author":[{"given":"Woranich","family":"Hinthong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9659-9085","authenticated-orcid":true,"given":"Varangkana","family":"Thaotumpitak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4267-2661","authenticated-orcid":true,"given":"Jarukorn","family":"Sripradite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitaya","family":"Indrawattana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thassanee","family":"Srisook","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thida","family":"Kongngoen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"R. 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