{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T14:48:19Z","timestamp":1766760499857,"version":"3.40.5"},"reference-count":76,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T00:00:00Z","timestamp":1705622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Jiangsu Provincial Postgraduate Scientific Practice and Innovation Project","award":["No. SJCX21_1722"],"award-info":[{"award-number":["No. SJCX21_1722"]}]},{"name":"The Sichuan Science and Technology Program","award":["No.2020YJ0492"],"award-info":[{"award-number":["No.2020YJ0492"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>With the emergence of penicillin resistance, the development of novel antibiotics has become an urgent necessity. Semi-synthetic penicillin has emerged as a promising alternative to traditional penicillin. The demand for the crucial intermediate, 6-aminopicillanic acid (6-APA), is on the rise. Enzyme catalysis is the primary method employed for its production. However, due to certain limitations, the strategy of enzyme immobilization has also gained prominence. The magnetic Ni<jats:sub>0.4<\/jats:sub>Cu<jats:sub>0.5<\/jats:sub>Zn<jats:sub>0.1<\/jats:sub>Fe<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub> nanoparticles were successfully prepared by a rapid-combustion method. Sodium silicate was used to modify the surface of the Ni<jats:sub>0.4<\/jats:sub>Cu<jats:sub>0.5<\/jats:sub>Zn<jats:sub>0.1<\/jats:sub>Fe<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub> nanoparticles to obtain silica-coated nanoparticles (Ni<jats:sub>0.4<\/jats:sub>Cu<jats:sub>0.5<\/jats:sub>Zn<jats:sub>0.1<\/jats:sub>Fe<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>-SiO<jats:sub>2<\/jats:sub>). Subsequently, in order to better crosslink PGA, the nanoparticles were modified again with glutaraldehyde to obtain glutaraldehyde crosslinked Ni<jats:sub>0.4<\/jats:sub>Cu<jats:sub>0.5<\/jats:sub>Zn<jats:sub>0.1<\/jats:sub>Fe<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>-SiO<jats:sub>2<\/jats:sub>-GA nanoparticles which could immobilize the PGA. The structure of the PGA protein was analyzed by the PyMol program and the immobilization strategy was determined. The conditions of PGA immobilization were investigated, including immobilization time and PGA concentration. Finally, the enzymological properties of the immobilized and free PGA were compared. The optimum catalytic pH of immobilized and free PGA was 8.0, and the optimum catalytic temperature of immobilized PGA was 50\u00b0C, 5\u00b0C higher than that of free PGA. Immobilized PGA in a certain pH and temperature range showed better catalytic stability. V<jats:sub>max<\/jats:sub> and K<jats:sub>m<\/jats:sub> of immobilized PGA were 0.3727 \u03bcmol\u00b7min<jats:sup>-1<\/jats:sup> and 0.0436 mol\u00b7L<jats:sup>-1<\/jats:sup>, and the corresponding free PGA were 0.7325 \u03bcmol\u00b7min<jats:sup>-1<\/jats:sup> and 0.0227 mol\u00b7L<jats:sup>-1<\/jats:sup>. After five cycles, the immobilized enzyme activity was still higher than 25%.<\/jats:p>","DOI":"10.1371\/journal.pone.0297149","type":"journal-article","created":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T18:46:39Z","timestamp":1705689999000},"page":"e0297149","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Enhanced catalytic performance of penicillin G acylase by covalent immobilization onto functionally-modified magnetic Ni0.4Cu0.5Zn0.1Fe2O4 nanoparticles"],"prefix":"10.1371","volume":"19","author":[{"given":"Zhixiang","family":"Lv","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhou","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0371-1599","authenticated-orcid":true,"given":"Shaobo","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,1,19]]},"reference":[{"key":"pone.0297149.ref001","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.3390\/antibiotics11101336","article-title":"Multidisciplinary Collaboration for the Optimization of Antibiotic Prescription: Analysis of Clinical Cases of Pneumonia between Emergency, Internal Medicine, and Pharmacy Services.","volume":"11","author":"L Arteche-Eguizabal","year":"2022","journal-title":"Antibiotics."},{"key":"pone.0297149.ref002","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1007\/s11250-017-1290-z","article-title":"Assessment of antibiotic use in farm animals in Rwanda.","volume":"49","author":"R Manishimwe","year":"2017","journal-title":"Trop Anim Health Prod"},{"key":"pone.0297149.ref003","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1146\/annurev.phyto.40.120301.093927","article-title":"Antibiotic use in plant agriculture","volume":"40","author":"PS McManus","year":"2002","journal-title":"Annu Rev Phytopathol"},{"key":"pone.0297149.ref004","doi-asserted-by":"crossref","first-page":"4561","DOI":"10.1021\/acs.jafc.3c01052","article-title":"Uptake of the Plant Agriculture-Used Antibiotics Oxytetracycline and Streptomycin by Cherry Radish\u2500Effect on Plant Microbiome and the Potential Health Risk","volume":"71","author":"LC Yin","year":"2023","journal-title":"J Agric Food Chem"},{"key":"pone.0297149.ref005","doi-asserted-by":"crossref","first-page":"3212","DOI":"10.1111\/1541-4337.13181","article-title":"Antibiotic resistance mechanism and diagnosis of common foodborne pathogens based on genotypic and phenotypic biomarkers.","volume":"22","author":"XY Liao","year":"2023","journal-title":"Compr Rev Food Sci Food Saf"},{"key":"pone.0297149.ref006","doi-asserted-by":"crossref","first-page":"356","DOI":"10.2174\/1381612829666221219093450","article-title":"Antibiotic Resistance and Microbiota Response.","volume":"29","author":"L Santacroce","year":"2023","journal-title":"Curr Pharm Des."},{"key":"pone.0297149.ref007","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1007\/s11427-022-2246-4","article-title":"A comprehensive review on natural phenolic compounds as alternatives to in-feed antibiotics","volume":"66","author":"JX Gao","year":"2023","journal-title":"Sci China Life Sci"},{"key":"pone.0297149.ref008","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.3390\/antibiotics12061037","article-title":"Biological Function of Antimicrobial Peptides on Suppressing Pathogens and Improving Host Immunity.","volume":"12","author":"ZQ Lyu","year":"2023","journal-title":"Antibiotics"},{"key":"pone.0297149.ref009","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1111\/j.1365-2672.2007.03599.x","article-title":"Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology","volume":"104","author":"YH Wang","year":"2008","journal-title":"J Appl Microbiol"},{"key":"pone.0297149.ref010","doi-asserted-by":"crossref","first-page":"2773","DOI":"10.1007\/s00253-013-4757-5","article-title":"Total synthesis of antibiotics: recent achievements, limitations, and perspectives","volume":"97","author":"EV Prusov","year":"2013","journal-title":"Appl Microbiol Biotechnol"},{"key":"pone.0297149.ref011","doi-asserted-by":"crossref","first-page":"4324","DOI":"10.3390\/molecules27134324","article-title":"Potent Antibiotic Lemonomycin: A Glimpse of Its Discovery, Origin, and Chemical Synthesis.","volume":"27","author":"SN Tao","year":"2022","journal-title":"Molecules"},{"key":"pone.0297149.ref012","doi-asserted-by":"crossref","first-page":"3855","DOI":"10.2174\/092986710793205435","article-title":"Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives","volume":"17","author":"G Volpato","year":"2010","journal-title":"Curr Med Chem"},{"key":"pone.0297149.ref013","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1016\/j.biotechadv.2013.05.006","article-title":"Biotechnological advances on Penicillin G acylase: Pharmaceutical implications, unique expression mechanism and production strategies","volume":"31","author":"K Srirangan","year":"2013","journal-title":"Biotechnol Adv"},{"key":"pone.0297149.ref014","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1039\/D2MD00412G","article-title":"Semisynthetic blasticidin S ester derivatives show enhanced antibiotic activity.","volume":"14","author":"C Gannett","year":"2023","journal-title":"RSC Med Chem"},{"key":"pone.0297149.ref015","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1021\/acsinfecdis.2c00253","article-title":"Recent Advances in the Development of Semisynthetic Glycopeptide Antibiotics: 2014\u20132022.","volume":"8","author":"E Van Groesen","year":"2022","journal-title":"ACS Infect Dis."},{"key":"pone.0297149.ref016","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.tetasy.2016.11.007","article-title":"Batch and in-flow kinetic resolution of racemic 1-(N-acylamino)alkylphosphonic and 1-(N-acylamino)alkylphosphinic acids and their esters using immobilized penicillin G acylase.","volume":"28","author":"K Zieli\u0144ska","year":"2017","journal-title":"Tetrahedron Asymmetry"},{"key":"pone.0297149.ref017","doi-asserted-by":"crossref","first-page":"303","DOI":"10.3109\/07388551.2014.960359","article-title":"Penicillin acylases revisited: importance beyond their industrial utility","volume":"36","author":"VS Avinash","year":"2016","journal-title":"Crit Rev Biotechnol"},{"key":"pone.0297149.ref018","doi-asserted-by":"crossref","first-page":"106533","DOI":"10.1016\/j.bioorg.2023.106533","article-title":"Enlarging the substrate binding pocket of penicillin G acylase from Achromobacter sp. for highly efficient biosynthesis of \u03b2-lactam antibiotics.","volume":"136","author":"Z Yan","year":"2023","journal-title":"Bioorganic Chem."},{"key":"pone.0297149.ref019","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.enzmictec.2018.08.006","article-title":"Directed evolution of a penicillin V acylase from Bacillus sphaericus to improve its catalytic efficiency for 6-APA production","volume":"119","author":"G Xu","year":"2018","journal-title":"Enzyme Microb Technol"},{"key":"pone.0297149.ref020","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ijantimicag.2007.11.010","article-title":"Development of the semi-synthetic penicillins and cephalosporins","volume":"31","author":"JMT Hamilton-Miller","year":"2008","journal-title":"Int J Antimicrob Agents"},{"key":"pone.0297149.ref021","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ijantimicag.2006.09.003","article-title":"The 50th anniversary of the discovery of 6-aminopenicillanic acid (6-APA).","volume":"29","author":"GN Rolinson","year":"2007","journal-title":"Int J Antimicrob Agents"},{"key":"pone.0297149.ref022","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1080\/10426507.2021.1946537","article-title":"Design and synthesis of novel 2,4,5-thiazole derivatives as 6-APA mimics and antimicrobial activity evaluation","volume":"196","author":"D Nuha","year":"2021","journal-title":"Phosphorus Sulfur Silicon Relat Elem"},{"key":"pone.0297149.ref023","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1007\/s13762-021-03397-w","article-title":"A novel approach for designing an industrial enzymatic process of 6-ammoniopenicillanic acid (6-APA).","volume":"19","author":"S Fereidouni Velasejerdi","year":"2022","journal-title":"Int J Environ Sci Technol"},{"key":"pone.0297149.ref024","doi-asserted-by":"crossref","first-page":"1158275","DOI":"10.3389\/fbioe.2023.1158275","article-title":"Editorial: Green chemistry biocatalysis","volume":"11","author":"GTM Henehan","year":"2023","journal-title":"Front Bioeng Biotechnol"},{"key":"pone.0297149.ref025","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1002\/pat.4791","article-title":"Recent progress in the development of immobilized penicillin G acylase for chemical and industrial applications: A mini\u2010review","volume":"31","author":"K Li","year":"2020","journal-title":"Polym Adv Technol"},{"key":"pone.0297149.ref026","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.procbio.2020.08.014","article-title":"Immobilization of esterase SulE in cross-linked gelatin\/chitosan and its application in remediating soils polluted with tribenuron-methyl and metsulfuron-methyl","volume":"98","author":"ZX Yu","year":"2020","journal-title":"Process Biochem"},{"key":"pone.0297149.ref027","doi-asserted-by":"crossref","first-page":"8404","DOI":"10.1021\/acs.langmuir.3c00362","article-title":"Immobilization of Laccase by Alkali-Etched Bimetallic CoCu-MOF To Enhance Enzyme Loading and Congo Red Degradation.","volume":"39","author":"HY Long","year":"2023","journal-title":"Langmuir"},{"key":"pone.0297149.ref028","first-page":"29","article-title":"An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes","author":"NR Mohamad","year":"2015","journal-title":"Biotechnol Biotechnol Equip"},{"key":"pone.0297149.ref029","doi-asserted-by":"crossref","first-page":"385101","DOI":"10.1088\/1361-6528\/acda35","article-title":"Nanotechnology and enzyme immobilization: a review","volume":"34","author":"MA Oke","year":"2023","journal-title":"Nanotechnology"},{"key":"pone.0297149.ref030","first-page":"64","article-title":"New insights into the effectiveness of alpha-amylase enzyme presentation on the Bacillus subtilis spore surface by adsorption and covalent immobilization","author":"F Gashtasbi","year":"2014","journal-title":"Enzyme Microb Technol"},{"key":"pone.0297149.ref031","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/j.rser.2018.04.103","article-title":"Progress & prospect of metal-organic frameworks (MOFs) for enzyme immobilization (enzyme\/MOFs).","volume":"91","author":"YL Hu","year":"2018","journal-title":"Renew Sustain Energy Rev."},{"key":"pone.0297149.ref032","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1002\/adsc.201800307","article-title":"Enzyme Immobilization on Inorganic Surfaces for Membrane Reactor Applications: Mass Transfer Challenges, Enzyme Leakage and Reuse of Materials","volume":"360","author":"SB Sigurdard\u00f3ttir","year":"2018","journal-title":"Adv Synth Catal"},{"key":"pone.0297149.ref033","doi-asserted-by":"crossref","first-page":"2100017","DOI":"10.1002\/star.202100017","article-title":"\u03b1\u2010Amylase Immobilization on Ceramic Membranes for Starch Hydrolysis","volume":"74","author":"EA Nazarova","year":"2022","journal-title":"Starch\u2014St\u00e4rke"},{"key":"pone.0297149.ref034","doi-asserted-by":"crossref","first-page":"129525","DOI":"10.1016\/j.jhazmat.2022.129525","article-title":"Gentle one-step co-precipitation to synthesize bimetallic CoCu-MOF immobilized laccase for boosting enzyme stability and Congo red removal","volume":"438","author":"XP Li","year":"2022","journal-title":"J Hazard Mater"},{"key":"pone.0297149.ref035","doi-asserted-by":"crossref","first-page":"1900437","DOI":"10.1002\/admi.201900437","article-title":"Nanoparticle\u2010Mediated Enzyme Immobilization on Cellulose Fibers: Reusable Biocatalytic Systems for Cascade Reactions","volume":"6","author":"C Goldhahn","year":"2019","journal-title":"Adv Mater Interfaces"},{"key":"pone.0297149.ref036","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jbiotec.2004.01.006","article-title":"Biospecific immobilization of mannan\u2013penicillin G acylase neoglycoenzyme on Concanavalin A-bead cellulose","volume":"110","author":"D Mislovi\u010dov\u00e1","year":"2004","journal-title":"J Biotechnol"},{"key":"pone.0297149.ref037","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1007\/s12257-009-0188-7","article-title":"Post-immobilization of modified macromolecular reagents using assembled penicillin acylase for microenvironmental regulation of nanopores and enhancement of enzyme stability","volume":"15","author":"C Zhou","year":"2010","journal-title":"Biotechnol Bioprocess Eng"},{"key":"pone.0297149.ref038","doi-asserted-by":"crossref","first-page":"151","DOI":"10.3390\/catal13010151","article-title":"Synergy of Ion Exchange and Covalent Reaction: Immobilization of Penicillin G Acylase on Heterofunctional Amino-Vinyl Sulfone Agarose.","volume":"13","author":"TN Da Rocha","year":"2023","journal-title":"Catalysts."},{"key":"pone.0297149.ref039","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.enzmictec.2010.07.010","article-title":"Evaluation of the incidence of diffusional restrictions on the enzymatic reactions of hydrolysis of penicillin G and synthesis of cephalexin","volume":"47","author":"P Valencia","year":"2010","journal-title":"Enzyme Microb Technol"},{"key":"pone.0297149.ref040","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1002\/ceat.201100297","article-title":"Hydrolysis of Penicillin G by Penicillin G Acylase Immobilized on Chitosan Microbeads in Different Reactor Systems","volume":"34","author":"MG \u017du\u017ea","year":"2011","journal-title":"Chem Eng Technol"},{"key":"pone.0297149.ref041","doi-asserted-by":"crossref","first-page":"7344","DOI":"10.1016\/j.biortech.2010.04.036","article-title":"Production of butyl-biodiesel using lipase physically-adsorbed onto electrospun polyacrylonitrile fibers","volume":"101","author":"SJ Sakai","year":"2010","journal-title":"Bioresour Technol"},{"key":"pone.0297149.ref042","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1002\/app.11154","article-title":"A polyacrylic acid as a carrier for immobilization of penicillin acylase","volume":"86","author":"YX Li","year":"2002","journal-title":"J Appl Polym Sci"},{"key":"pone.0297149.ref043","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.chroma.2016.02.070","article-title":"Preparation and evaluation of dual-enzyme microreactor with co-immobilized trypsin and chymotrypsin","volume":"1440","author":"K Meller","year":"2016","journal-title":"J Chromatogr A"},{"key":"pone.0297149.ref044","doi-asserted-by":"crossref","first-page":"6587","DOI":"10.1039\/C5TB00870K","article-title":"MOF-templated rough, ultrathin inorganic microcapsules for enzyme immobilization","volume":"3","author":"XL Wang","year":"2015","journal-title":"J Mater Chem B"},{"key":"pone.0297149.ref045","doi-asserted-by":"crossref","first-page":"4285","DOI":"10.1021\/acsami.1c20738","article-title":"Selective Coimmobilization of His-Tagged Enzymes on Yttrium-Stabilized Zirconia-Based Membranes for Continuous Asymmetric Bioreductions.","volume":"14","author":"D Andr\u00e9s-Sanz","year":"2022","journal-title":"ACS Appl Mater Interfaces."},{"key":"pone.0297149.ref046","doi-asserted-by":"crossref","first-page":"907855","DOI":"10.3389\/fbioe.2022.907855","article-title":"Surface Functionalization of SBA-15 for Immobilization of Myoglobin.","volume":"10","author":"HM Miao","year":"2022","journal-title":"Front Bioeng Biotechnol."},{"key":"pone.0297149.ref047","doi-asserted-by":"crossref","first-page":"2160","DOI":"10.1002\/adfm.200600505","article-title":"Functionalization of Cubic I a3d Mesoporous Silica for Immobilization of Penicillin G Acylase","volume":"17","author":"Y L\u00fc","year":"2007","journal-title":"Adv Funct Mater"},{"key":"pone.0297149.ref048","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1016\/S1872-2067(14)60156-X","article-title":"Epoxidation of vinyl functionalized cubic Ia3d mesoporous silica for immobilization of penicillin G acylase","volume":"35","author":"WC Zhan","year":"2014","journal-title":"Chin J Catal"},{"key":"pone.0297149.ref049","doi-asserted-by":"crossref","first-page":"1108820","DOI":"10.3389\/fbioe.2023.1108820","article-title":"Immobilization and property of penicillin G acylase on amino functionalized magnetic Ni0.3Mg0.4Zn0.3Fe2O4 nanoparticles prepared via the rapid combustion process.","volume":"11","author":"MY Ma","year":"2023","journal-title":"Front Bioeng Biotechnol"},{"key":"pone.0297149.ref050","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1002\/mabi.200700256","article-title":"Immobilization of Penicillin G Acylase on Poly[(glycidyl methacrylate)-co-(glycerol monomethacrylate)]-Grafted Magnetic Microspheres.","volume":"8","author":"J Huang","year":"2008","journal-title":"Macromol Biosci."},{"key":"pone.0297149.ref051","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.enzmictec.2014.03.011","article-title":"Immobilization of penicillin G acylase on paramagnetic aldehyde-functionalized mesostructured cellular foams","volume":"60","author":"L Yang","year":"2014","journal-title":"Enzyme Microb Technol"},{"key":"pone.0297149.ref052","first-page":"103","article-title":"The study of Fe3O4@SiO2-NH2 nano-magnetic composite modified by glutaraldehyde to immobilized penicillin G acylase.","volume":"46","author":"MAA Mohammed","year":"2022","journal-title":"Turk J Chem"},{"key":"pone.0297149.ref053","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1080\/09205063.2021.2021352","article-title":"Comparative study of poly tannic acid functionalized magnetic particles before and after modification for immobilized penicillin G acylase","volume":"33","author":"HY Tu","year":"2022","journal-title":"J Biomater Sci Polym Ed"},{"key":"pone.0297149.ref054","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.aca.2017.12.022","article-title":"Tyrosinase immobilization on aminated magnetic nanoparticles by physical adsorption combined with covalent crosslinking with improved catalytic activity, reusability and storage stability","volume":"1006","author":"DM Liu","year":"2018","journal-title":"Anal Chim Acta"},{"key":"pone.0297149.ref055","doi-asserted-by":"crossref","first-page":"113286","DOI":"10.1016\/j.colsurfb.2023.113286","article-title":"Performance of microenvironment-induced lipase immobilization on diversify surface of magnetic particle","volume":"225","author":"MX Luo","year":"2023","journal-title":"Colloids Surf B Biointerfaces"},{"key":"pone.0297149.ref056","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.ijbiomac.2017.07.045","article-title":"\u03b1-Glucosidase immobilization on chitosan-enriched magnetic composites for enzyme inhibitors screening","volume":"105","author":"DM Liu","year":"2017","journal-title":"Int J Biol Macromol"},{"key":"pone.0297149.ref057","article-title":"Zn and Co ferrite nanoparticles: towards the applications of sensing and adsorption studies.","author":"LV Hublikar","year":"2023","journal-title":"Environ Sci Pollut R."},{"key":"pone.0297149.ref058","first-page":"225","article-title":"Synthesis and comparison of three photocatalysts for degrading tramadol as an analgesic and widely used drug in water samples","author":"F Kazemi","year":"2023","journal-title":"Environ Res"},{"key":"pone.0297149.ref059","first-page":"116241","article-title":"Degradation of cefadroxil drug by newly designed solar light responsive alcoholic template-based lanthanum ferrite nanoparticles.","volume":"231","author":"A Nazir","year":"2023"},{"issue":"7","key":"pone.0297149.ref060","doi-asserted-by":"crossref","DOI":"10.1002\/aoc.7110","article-title":"Green-synthesis of Ni-Co nanoferrites using aloe vera extract: Structural, optical, magnetic, and antimicrobial studies","volume":"37","author":"N Dhanda","year":"2023","journal-title":"Appl Organomet Chem"},{"key":"pone.0297149.ref061","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.jksus.2023.102708","article-title":"Anticancer potentiality of green synthesized Mg-Co ferrites nanoparticles against human breast cancer MCF-7 cells.","volume":"35","author":"N Ahmad","year":"2023","journal-title":"J King Saud Univ Sci."},{"key":"pone.0297149.ref062","first-page":"22","article-title":"PEGylated Manganese-Zinc Ferrite Nanocrystals Combined with Intratumoral Implantation of Micromagnets Enabled Synergetic Prostate Cancer Therapy via Ferroptotic and Immunogenic Cell Death.","volume":"19","author":"H Wang","year":"2023","journal-title":"Small"},{"key":"pone.0297149.ref063","doi-asserted-by":"crossref","first-page":"9838","DOI":"10.3390\/ijms24129838","article-title":"Thermally Stable and Reusable Silica and Nano-Fructosome Encapsulated CalB Enzyme Particles for Rapid Enzymatic Hydrolysis and Acylation.","volume":"24","author":"WY Jang","year":"2023","journal-title":"Int J Mol Sci"},{"key":"pone.0297149.ref064","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.procbio.2023.02.024","article-title":"Fabrication of Fe3O4@SiO2@PDA-Ni2+ nanoparticles for one-step affinity immobilization and purification of His-tagged glucose dehydrogenase","volume":"128","author":"JY Huang","year":"2023","journal-title":"Process Biochem"},{"key":"pone.0297149.ref065","doi-asserted-by":"crossref","first-page":"404","DOI":"10.3390\/molecules28010404","article-title":"Development of Pectinase Based Nanocatalyst by Immobilization of Pectinase on Magnetic Iron Oxide Nanoparticles Using Glutaraldehyde as Crosslinking Agent.","volume":"28","author":"T Behram","year":"2023","journal-title":"Molecules"},{"key":"pone.0297149.ref066","doi-asserted-by":"crossref","first-page":"124406","DOI":"10.1016\/j.talanta.2023.124406","article-title":"Nanoassemblies of acetylcholinesterase and \u03b2-lactamase immobilized on magnetic nanoparticles as biosensors to detect pollutants in water.","volume":"258","author":"M Jimenez-Carretero","year":"2023","journal-title":"Talanta."},{"key":"pone.0297149.ref067","doi-asserted-by":"crossref","first-page":"115899","DOI":"10.1016\/j.envres.2023.115899","article-title":"Synthesis of magnetically recyclable porous cross-linked aggregates of Tramates versicolor MTCC 138 laccase for the efficient removal of pentachlorophenol from aqueous solution","volume":"229","author":"S Venkataraman","year":"2023","journal-title":"Environ Res"},{"key":"pone.0297149.ref068","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.ijbiomac.2019.05.066","article-title":"Covalent immobilization and characterization of penicillin G acylase on amino and GO functionalized magnetic Ni0.5Zn0.5Fe2O4@SiO2 nanocomposite prepared via a novel rapid-combustion process","volume":"134","author":"Q Yu","year":"2019","journal-title":"Int J Biol Macromol"},{"key":"pone.0297149.ref069","doi-asserted-by":"crossref","first-page":"123248","DOI":"10.1016\/j.ijbiomac.2023.123248","article-title":"Synthesis, modification and application of fish skin gelatin-based hydrogel as sustainable and versatile bioresource of antidiabetic peptide","volume":"231","author":"HSH Munawaroh","year":"2023","journal-title":"Int J Biol Macromol"},{"key":"pone.0297149.ref070","doi-asserted-by":"crossref","first-page":"130609","DOI":"10.1016\/j.colsurfa.2022.130609","article-title":"Synthesis of polymer-functionalized \u03b2-cyclodextrin, Mg2+ doped, coating magnetic Fe3O4 nanoparticle carriers for penicillin G acylase immobilization.","volume":"657","author":"G Wu","year":"2023","journal-title":"Colloids Surf Physicochem Eng Asp"},{"key":"pone.0297149.ref071","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1006\/jmbi.2001.5043","article-title":"Crystal structures of penicillin acylase enzyme-substrate complexes: structural insights into the catalytic mechanism 1 1Edited by K. Nagai","volume":"313","author":"CE McVey","year":"2001","journal-title":"J Mol Biol"},{"key":"pone.0297149.ref072","doi-asserted-by":"crossref","first-page":"140353","DOI":"10.1016\/j.cplett.2023.140353","article-title":"Dihydroflavonol 4-reductase immobilized on Fe3O4-chitosan nanoparticles as a nano-biocatalyst for synthesis of anthocyanidins","volume":"815","author":"XF Li","year":"2023","journal-title":"Chem Phys Lett"},{"key":"pone.0297149.ref073","doi-asserted-by":"crossref","first-page":"966","DOI":"10.3390\/ijms21030966","article-title":"Laccase Properties, Physiological Functions, and Evolution","volume":"21","author":"G Janusz","year":"2020","journal-title":"Int J Mol Sci"},{"key":"pone.0297149.ref074","doi-asserted-by":"crossref","first-page":"128688","DOI":"10.1016\/j.jhazmat.2022.128688","article-title":"Bioremoval of estrogens by laccase immobilized onto polyacrylonitrile\/polyethersulfone material: Effect of inhibitors and mediators, process characterization and catalytic pathways determination","volume":"432","author":"J Zdarta","year":"2022","journal-title":"J Hazard Mater"},{"key":"pone.0297149.ref075","doi-asserted-by":"crossref","first-page":"132714","DOI":"10.1016\/j.jhazmat.2023.132714","article-title":"Covalent organic framework in-situ immobilized laccase for the covalent polymerization removal of sulfamethoxazole in the presence of natural phenols: Prominent enzyme stability and activity","volume":"462","author":"JH Xu","year":"2024","journal-title":"J Hazard Mater"},{"key":"pone.0297149.ref076","doi-asserted-by":"crossref","first-page":"136638","DOI":"10.1016\/j.molstruc.2023.136638","article-title":"Simple separation operation improved enzyme-catalyzed e-caprolactone yield","volume":"1295","author":"WY Zhang","year":"2024","journal-title":"J Mol Struct"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0297149","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T18:47:10Z","timestamp":1705690030000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0297149"}},"subtitle":[],"editor":[{"given":"Cinzia","family":"Calvio","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,1,19]]},"references-count":76,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0297149","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2024,1,19]]}}}