{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T11:46:02Z","timestamp":1788176762899,"version":"build-2803163510"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T00:00:00Z","timestamp":1705622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Objective<\/jats:title>\n<jats:p>To determine trends in incidence, etiology and antimicrobial susceptibility of blood and cerebrospinal fluid (CSF) culture confirmed infections in hospitalized infants in a large tertiary neonatal unit in South Africa.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Single-center, retrospective review of laboratory records of bacteria and fungi, and their susceptibility profiles, isolated from blood and CSF of infants hospitalized in the neonatal unit at Chris Hani Baragwanath Academic Hospital, Johannesburg, South Africa, from 1<jats:sup>st<\/jats:sup> January 2010 to 31<jats:sup>st<\/jats:sup> December 2019. Laboratory data on isolates and their antimicrobial susceptibilities were collected. Coagulase-negative <jats:italic>Staphylococcus<\/jats:italic>, <jats:italic>Corynebacteria<\/jats:italic> and <jats:italic>Bacillus spp<\/jats:italic>. were excluded. Patient-level clinical and laboratory data were not available.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>There were 8,319 significant isolates, giving an infection rate of 14.3\/1000 patient-days. Infection rates increased from 12.0 to 15.7\/1000 patient-days (estimated average yearly change 0.6[95%CI, 0.5\u20130.7];p = &lt;0.001). Gram-negative infection rates increased from 4.3 to 10.8\/1000 patient-days (estimated average yearly change 0.7[95%CI,0.6\u20130.8];p = &lt;0.001). The 2 most commonly isolated Gram-negative organisms were <jats:italic>Acinetobacter baumannii<\/jats:italic> (44%) and <jats:italic>Klebsiella pneumoniae<\/jats:italic> (39%). Carbapenem resistance was seen in 31% of all Gram-negatives and increased over time (estimated average yearly change 4.8%[95%CI,4.2%-5.3%];p&lt;0.001). Gram-positive infection rates decreased (estimated average yearly change -0.1[95%CI,-0.2\u2013 -0.05];p = &lt;0.001). <jats:italic>Staphylococcus aureus<\/jats:italic> was the most common Gram-positive isolated. Rates of methicillin-resistant <jats:italic>Staphylococcus aureus<\/jats:italic> decreased from 91% to 55%(estimated average yearly change -2.8%[95%CI,-3.5%\u20132%],p&lt; 0.001). Rates of fungal isolates decreased (estimated average yearly change -0.06[95%CI,-0.1 \u2013-0.02]);p = 0.007). <jats:italic>Candida parapsilosis<\/jats:italic> (52%) and <jats:italic>Candida albicans<\/jats:italic> (35%) were the most common fungi isolated.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>There has been a marked overall increase in rates of blood and\/or CSF infections, with an absolute increase in Gram-negative infections observed, replacing Gram-positive and fungal pathogens. Extended spectrum beta-lactamase Gram-negative isolates are being replaced by carbapenem resistance, with around one third of all significant Gram-negative isolates now carbapenem resistant. Research into hospital based novel treatment and prevention interventions for neonatal sepsis should be urgently prioritized.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0297371","type":"journal-article","created":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T18:46:22Z","timestamp":1705689982000},"page":"e0297371","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Incidence, pathogens and antimicrobial resistance of blood and cerebrospinal fluid isolates from a tertiary neonatal unit in South Africa: A 10 year retrospective review"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5931-0850","authenticated-orcid":true,"given":"Reenu","family":"Thomas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Ondongo-Ezhet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nini","family":"Motsoaledi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Sharland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Clements","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sithembiso","family":"Velaphi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,1,19]]},"reference":[{"key":"pone.0297371.ref001","article-title":"Global incidence and mortality of neonatal sepsis: a systematic review and meta-analysis","author":"C Fleischmann","year":"2021","journal-title":"Arch Dis Child"},{"key":"pone.0297371.ref002","unstructured":"World Health Organization. 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