{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:02:18Z","timestamp":1787022138698,"version":"build-2736575974"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T00:00:00Z","timestamp":1709769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007943","name":"Graduate School, Kasetsart University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007943","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["220211"],"award-info":[{"award-number":["220211"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Rickettsiosis is caused by\n                    <jats:italic>Orientia<\/jats:italic>\n                    spp. and\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    spp., arthropod-borne zoonotic intracellular bacteria. The close relationships between pet dogs, cats and owners increase the risk of rickettsial transmission, with limited studies on the seroprevalence in pets. This study investigated the prevalence of rickettsia exposure among dogs and cats in Bangkok and neighboring provinces. The samples from 367 dogs and 187 cats used in this study were leftover serum samples from routine laboratory testing stored at the Veterinary Teaching Hospital. In-house Enzyme-linked immunosorbent assay (ELISA) tests included IgG against the scrub typhus group (STG), typhus group (TG), and spotted fever group (SFG). The seroprevalence in pet dogs was 30.25% (111\/367), including 21.53% for STG, 4.36% for TG, and 1.09% for SFG. Co-seroprevalence consisted of 2.72% for STG and TG, 0.27% for STG and SFG, and 0.27% for pangroup infection. The prevalence in cats was 62.56% (117\/187), including 28.34% for STG, 4.28% for TG, and 6.42% for STG. Co-seroprevalence in cats consisted of STG and TG (4.28%), STG and SFG (5.35%), TG and SFG (3.21%), and three-group infection (10.69%). No significant difference in seroprevalence for the three serogroups was observed in any of the 64 districts sampled. The mean hematocrit level significantly decreased in seropositive dogs (P&lt;0.05). Seropositive dogs and cats were detected in significantly greater numbers of anemia cases than nonanemia cases (P&lt;0.05) (odds ratio: 7.93, 0.44, p = 0.00, p = 0.01). A significantly higher number of seropositive cats had decreased hemoglobin levels (P&lt;0.05) (odds ratio: 3.63, p = 0.00). The seropositive samples significantly differed among older cats (P&lt;0.05). These high exposures in pet dogs and cats could constitute important relationship dynamics between companion animals and rickettsial vectors. Significantly decreased hematocrit and hemoglobin levels indicated anemia in the exposed dogs and cats. The study findings will raise awareness of this neglected disease among pet owners and veterinary hospital personnel and aid in future public health preventative planning.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0297373","type":"journal-article","created":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T13:50:29Z","timestamp":1709819429000},"page":"e0297373","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Serological detection of Rickettsia spp. and evaluation of blood parameters in pet dogs and cats from Bangkok and neighboring 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Zoonotic Dis."},{"key":"pone.0297373.ref012","first-page":"188","article-title":"Cats as potential mammalian reservoirs for Rickettsia sp. genotype RF2125 in Bangkok","volume":"13","author":"P Phoosangwalthong","year":"2018","journal-title":"Thailand. 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RF2125","volume":"4","author":"\u0160 \u0160lapeta","year":"2016","journal-title":"Vet Parasitol Reg Stud Reports"},{"issue":"3","key":"pone.0297373.ref034","doi-asserted-by":"crossref","first-page":"391","DOI":"10.3390\/pathogens12030391","article-title":"Companion Vector-Borne Pathogens and Associated Risk Factors in Apparently Healthy Pet Animals (Dogs and Cats) in Khukhot City Municipality, Pathum Thani Province, Thailand.","volume":"12","author":"NH Luong","year":"2023","journal-title":"Pathogens."},{"key":"pone.0297373.ref035","first-page":"5","article-title":"Cat tracking program makes owners re-think pets\u2019 behaviour and how they manage their moggies Australia","volume":"2023","author":"M. Pearce","year":"2016","journal-title":"ABC Central West"},{"issue":"10","key":"pone.0297373.ref036","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1046\/j.1469-0691.2002.00485.x","article-title":"Toxoplasma gondii: transmission, diagnosis and prevention","volume":"8","author":"D Hill","year":"2002","journal-title":"Clin Microbiol Infect"},{"issue":"3","key":"pone.0297373.ref037","doi-asserted-by":"crossref","first-page":"389","DOI":"10.3201\/eid1203.050931","article-title":"Bartonella spp. in pets and effect on human health","volume":"12","author":"BB Chomel","year":"2006","journal-title":"Emerg Infect Dis"},{"issue":"4","key":"pone.0297373.ref038","doi-asserted-by":"crossref","first-page":"20180840","DOI":"10.1098\/rsbl.2018.0840","article-title":"Who let the cats out? A global meta-analysis on risk of parasitic infection in indoor versus outdoor domestic cats (Felis catus).","volume":"15","author":"K Chalkowski","year":"2019","journal-title":"Biol Lett."},{"issue":"4","key":"pone.0297373.ref039","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S1471-4922(03)00027-8","article-title":"Toxocara cati: an underestimated zoonotic agent","volume":"19","author":"M. Fisher","year":"2003","journal-title":"Trends Parasitol"},{"key":"pone.0297373.ref040","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.applanim.2015.07.002","article-title":"A review of cat behavior in relation to disease risk and management options","volume":"173","author":"CA Lepczyk","year":"2015","journal-title":"Appl Anim Behav Sci"},{"issue":"12","key":"pone.0297373.ref041","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1136\/vr.101222","article-title":"Risk behaviours exhibited by free-roaming cats in a suburban US town","volume":"173","author":"KA Loyd","year":"2013","journal-title":"Vet Rec"},{"issue":"2","key":"pone.0297373.ref042","first-page":"98","article-title":"Progress on research on rodents and rodent-borne zoonoses","volume":"42","author":"K Blasdell","year":"2015","journal-title":"South-east Asia. Wildl. Res"},{"issue":"1","key":"pone.0297373.ref043","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1089\/vbz.2014.1629","article-title":"Aboriginal and invasive rats of genus Rattus as hosts of infectious agents.","volume":"15","author":"M Kosoy","year":"2015","journal-title":"Vector Borne Zoonotic Dis."},{"issue":"4","key":"pone.0297373.ref044","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1111\/mec.12149","article-title":"Cytonuclear discordance among Southeast Asian black rats (Rattus rattus complex).","volume":"22","author":"M Pages","year":"2013","journal-title":"Mol Ecol"},{"key":"pone.0297373.ref045","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ijppaw.2020.07.015","article-title":"Rickettsiae exposure related to habitats of the oriental house rat (Rattus tanezumi, Temminck, 1844) in Salaya suburb, Thailand.","volume":"13","author":"P Prompiram","year":"2020","journal-title":"Int J Parasitol Parasites Wildl"},{"issue":"6","key":"pone.0297373.ref046","first-page":"1071","article-title":"Infection of the endothelium by members of the order Rickettsiales","volume":"102","author":"G Valbuena","year":"2009","journal-title":"Thromb Haemost"},{"issue":"5","key":"pone.0297373.ref047","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/nrmicro1866","article-title":"Emerging and re-emerging rickettsioses: endothelial cell infection and early disease events","volume":"6","author":"DH Walker","year":"2008","journal-title":"Nat Rev Microbiol"},{"issue":"1","key":"pone.0297373.ref048","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jemermed.2018.02.043","article-title":"The evaluation and management of Rocky Mountain spotted fever in the emergency department: a review of the literature.","volume":"55","author":"M Gottlieb","year":"2018","journal-title":"J Emerg Med.Jul"},{"key":"pone.0297373.ref049","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.actatropica.2016.10.018","article-title":"Clinical and laboratory characteristics, epidemiology, and outcomes of murine typhus: a systematic review","volume":"166","author":"C Tsioutis","year":"2017","journal-title":"Acta Trop"},{"issue":"1","key":"pone.0297373.ref050","doi-asserted-by":"crossref","first-page":"40","DOI":"10.4103\/0972-9062.308799","article-title":"Serologic evidence of rickettsial diseases associated with tick bites in workers of urban veterinary clinics.","volume":"57","author":"ALM Escarcega-Avila","year":"2020","journal-title":"J Vector Borne Dis."}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0297373","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T13:51:00Z","timestamp":1709819460000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0297373"}},"subtitle":[],"editor":[{"given":"Joshua","family":"Kamani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,3,7]]},"references-count":50,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,3,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0297373","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,7]]}}}