{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:37:02Z","timestamp":1790771822586,"version":"4.1.0"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The objective of this study was to develop pyrazolidine-3,5-dione derivatives with potential as environmentally friendly pesticides for pest control, specifically focusing on their efficacy as larvicidal agents. A novel one-pot synthesis of multicomponent pyrazolidine-3,5-dione derivatives (<jats:bold>1a-m<\/jats:bold>) was accomplished via the grindstone method using Cu(II)tyrosinase enzyme as a catalyst under mild reaction conditions, yielding 84%\u201396%. The synthesised derivatives (<jats:bold>1a-m<\/jats:bold>) were characterized using various spectroscopic methods (mass spectrometry, elemental analysis, FT-IR, and <jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C NMR). NMR characterisation using DMSO-<jats:italic>d<\/jats:italic><jats:sub>6<\/jats:sub> as a solvent. The larvicidal and antifeedant activities of the synthesised compounds were screened and <jats:italic>in silico<\/jats:italic> computational studies were performed. The larvicidal activity against <jats:italic>Culex quinquefasciatus<\/jats:italic> and antifeedant activity against <jats:italic>Oreochromis mossambicus<\/jats:italic> were evaluated. Among the synthesised compounds, compound <jats:bold>1c<\/jats:bold> demonstrated superior efficacy (LD<jats:sub>50<\/jats:sub>: 9.7 \u03bcg\/mL) against <jats:italic>C<\/jats:italic>. <jats:italic>quinquefasciatus<\/jats:italic> compared to permethrin (LD<jats:sub>50<\/jats:sub>: 17.1 \u03bcg\/mL). Regarding antifeedant activity, compounds <jats:bold>1a<\/jats:bold>, <jats:bold>1e<\/jats:bold>, <jats:bold>1f<\/jats:bold>, <jats:bold>1j<\/jats:bold>, and <jats:bold>1k<\/jats:bold> exhibited 100% mortality at 100 \u03bcg\/mL. Molecular docking analysis was performed to assess the binding capacity of a mosquito odorant-binding protein (3OGN) from <jats:italic>Culex quinquefasciatus<\/jats:italic> to compound <jats:bold>1c<\/jats:bold>. The results revealed that compound <jats:bold>1c<\/jats:bold> had a docking score of -10.4 kcal\/mol, surpassing that of standard permethrin (-9.5 kcal\/mol). Furthermore, DFT calculations were conducted to acquire theoretical data aligned with the experimental FT-IR results. According to experimental research, compound <jats:bold>1c<\/jats:bold> demonstrates promising larvicidal activity against mosquito larvae of <jats:italic>C<\/jats:italic>. <jats:italic>quinquefasciatus<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pone.0298232","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T17:31:36Z","timestamp":1726767096000},"page":"e0298232","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cu(II)-tyrosinase enzyme catalyst mediated synthesis of\u00a0mosquito\u00a0larvicidal\u00a0active pyrazolidine-3,5-dione derivatives with molecular docking studies and their ichthyotoxicity analysis"],"prefix":"10.1371","volume":"19","author":[{"given":"Velmurugan","family":"Loganathan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SurendraKumar","family":"Radhakrishnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anis","family":"Ahamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raman","family":"Gurusamy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7351-813X","authenticated-orcid":true,"given":"Omar","family":"H. 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