{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T01:05:58Z","timestamp":1793408758360,"version":"4.5.0"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:00:00Z","timestamp":1710374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019789","name":"Punjab Agriculture Research Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019789","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018998","name":"IReL","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100018998","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\u03b2-glucan is a well-documented feed additive for its potent immunostimulatory properties in many farmed fish species. This study examined how it can also be a promising growth promoter, modulate antioxidant enzyme activities, and act as an anti-stress agent in striped catfish (<jats:italic>Pangasianodon hypophthalmus<\/jats:italic>). A 12-week feeding experiment was untaken to determine the effects of dietary \u03b2-glucan supplementation at graded levels (0, 0.5, 1.0, and 1.5 g kg<jats:sup>-1<\/jats:sup>). Measured indicators suggest that a dietary inclusion level of 1.5 g kg<jats:sup>-1<\/jats:sup> \u03b2-glucan gave the highest positive responses: weight gain (120.10 g fish<jats:sup>-1<\/jats:sup>), survival (98.30%), and lower FCR (1.70) (<jats:italic>P<\/jats:italic>&lt;0.05). Whole body proximate analysis had only revealed that crude protein was significantly affected by the dietary inclusion of \u03b2-glucan (<jats:italic>P<\/jats:italic>&lt;0.05), with the highest protein content (19.70%) being in fish that were fed with 1.5 g kg<jats:sup>-1<\/jats:sup> \u03b2-glucan. Although other inclusion levels (i.e., 0.5 and 1 g kg<jats:sup>-1<\/jats:sup>) of \u03b2-glucan did not enhance body protein content (<jats:italic>P<\/jats:italic>&gt;0.05). The assessment of fatty acid composition in muscle, liver, and adipose tissues showed modifications with the inclusion of \u03b2-glucan. Antioxidative-related enzyme activities (inc. catalase, glutathione peroxidase, and superoxide dismutase) that were measured in the liver had higher levels when fed with \u03b2-glucan inclusion diets (<jats:italic>P<\/jats:italic>&lt;0.05). Following the feed trial, fish were subjected to crowding stress treatment. It was subsequently found that catfish fed with \u03b2-glucan-based diet groups had lower levels of blood stress-related indicators compared to the control group with no dietary \u03b2-glucan. The use of 1.5 g kg<jats:sup>-1<\/jats:sup> of dietary \u03b2-glucan resulted in the lowest measured levels of cortisol (43.13 ng mL<jats:sup>-1<\/jats:sup>) and glucose (50.16 mg dL<jats:sup>-1<\/jats:sup>). This study has demonstrated that the dietary inclusion of \u03b2-glucan can have functional benefits beyond the immunological enhancements in striped catfish. Furthermore, its use can increase production levels and mitigate the stress associated with intensive farming practices.<\/jats:p>","DOI":"10.1371\/journal.pone.0298414","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T17:37:36Z","timestamp":1710437856000},"page":"e0298414","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Enhancing farmed striped catfish (Pangasianodon hypophthalmus) robustness through dietary \u03b2-glucan"],"prefix":"10.1371","volume":"19","author":[{"given":"Sheeza","family":"Bano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noor","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahroze","family":"Fatima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjum","family":"Khalique","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9865-6256","authenticated-orcid":true,"given":"Murat","family":"Arslan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadia","family":"Nazir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Asghar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayesha","family":"Khizar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon John","family":"Davies","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1703-3725","authenticated-orcid":true,"given":"Alex H. 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