{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T17:10:42Z","timestamp":1787764242498,"version":"build-2784847793"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T00:00:00Z","timestamp":1709769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R03 AI139557"],"award-info":[{"award-number":["R03 AI139557"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Genetic screening of pools of mutants can reveal genetic determinants involved in complex biological interactions, processes, and systems. We previously constructed two single-gene deletion resources for <jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium 14028s in which kanamycin (Kan<jats:sup>R<\/jats:sup>) and chloramphenicol (Cam<jats:sup>R<\/jats:sup>) cassettes were used to replace non-essential genes. We have now used lambda-red recombination to convert the antibiotic cassettes in these resources into a tetracycline-resistant (Tet<jats:sup>R<\/jats:sup>) version where each mutant contains a different 21-base barcode flanked by Illumina Read1 and Read2 primer sequences. A motility assay of a pool of the entire library, followed by a single-tube processing of the bacterial pellet, PCR, and sequencing, was used to verify the performance of the barcoded Tet<jats:sup>R<\/jats:sup> collection. The new resource is useful for experiments with defined subsets of barcoded mutant strains where biological bottlenecks preclude high numbers of founder bacteria, such as in animal infections. The Tet<jats:sup>R<\/jats:sup> version of the library will also facilitate the construction of triple mutants by transduction. The resource of 6197 mutants covering 3490 genes is deposited at Biological and Emerging Infections Resources (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/beiresources.org\" xlink:type=\"simple\">beiresources.org<\/jats:ext-link>).<\/jats:p>","DOI":"10.1371\/journal.pone.0298419","type":"journal-article","created":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T18:49:27Z","timestamp":1709837367000},"page":"e0298419","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["A genome-wide collection of barcoded single-gene deletion mutants in Salmonella enterica serovar Typhimurium"],"prefix":"10.1371","volume":"19","author":[{"given":"Steffen","family":"Porwollik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiping","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prerak T.","family":"Desai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"McClelland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,3,7]]},"reference":[{"issue":"2","key":"pone.0298419.ref001","doi-asserted-by":"crossref","first-page":"62","DOI":"10.4161\/gmic.19141","article-title":"Innate immune response to Salmonella typhimurium, a model enteric pathogen","volume":"3","author":"P Broz","year":"2012","journal-title":"Gut Microbes"},{"key":"pone.0298419.ref002","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1146\/annurev.med.52.1.259","article-title":"Salmonella: a model for bacterial pathogenesis.","volume":"52","author":"ME Ohl","year":"2001","journal-title":"Annu Rev Med"},{"key":"pone.0298419.ref003","first-page":"261","article-title":"Of mice, calves, and men. 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