{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:07:37Z","timestamp":1740146857269,"version":"3.37.3"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["20200610"],"award-info":[{"award-number":["20200610"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Sulfurtransferases transfer of sulfur atoms from thiols to acceptors like cyanide. They are categorized as thiosulfate sulfurtransferases (TSTs) and 3-mercaptopyruvate sulfurtransferases (MSTs). TSTs transfer sulfur from thiosulfate to cyanide, producing thiocyanate. MSTs transfer sulfur from 3-mercaptopyruvate to cyanide, yielding pyruvate and thiocyanate. The present study aimed to isolate and characterize the sulfurtransferase <jats:italic>Fr<\/jats:italic>ST from <jats:italic>Frondihabitans<\/jats:italic> sp. PAMC28461 using biochemical and structural analyses. <jats:italic>Fr<\/jats:italic>ST exists as a dimer and can be classified as a TST rather than an MST according to sequence-based clustering and enzyme activity. Furthermore, the discovery of activity over a wide temperature range and the broad substrate specificity exhibited by <jats:italic>Fr<\/jats:italic>ST suggest promising prospects for its utilization in industrial applications, such as the detoxification of cyanide.<\/jats:p>","DOI":"10.1371\/journal.pone.0298999","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T17:30:30Z","timestamp":1711387830000},"page":"e0298999","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural and functional characterization of sulfurtransferase from Frondihabitans sp. 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